A critical review of methodologies for evaluating iron fertilizers based on iron reduction and uptake by strategy I plants

Under iron (Fe)-limited conditions, plants have developed strategies for acquiring this essential micronutrient. Several Fe sources have been studied as potential fertilizers, with Fe synthetic chelates being the most used to prevent and correct Fe chlorosis in crops. The determination of the activi...

Full description

Bibliographic Details
Authors: Arcas, Alejandra, Garate Ormaechea, Agustín, López Rayo, Sandra, Lucena Marotta, Juan José
Format: article
Publication Date:2024
Country:España
Institution:Universidad Autónoma de Madrid
Repository:Biblos-e Archivo. Repositorio Institucional de la UAM
Language:English
OAI Identifier:oai:repositorio.uam.es:10486/715237
Online Access:http://hdl.handle.net/10486/715237
https://dx.doi.org/10.3390/plants13060819
Access Level:Open access
Keyword:57 Fe
chlorosis
Fe chelate reductase
Fe uptake
iron deficiency
Strategy I
Química
id ES_cdc2a9b06b3882bbe85de533a24b98fc
oai_identifier_str oai:repositorio.uam.es:10486/715237
network_acronym_str ES
network_name_str España
repository_id_str
spelling A critical review of methodologies for evaluating iron fertilizers based on iron reduction and uptake by strategy I plantsArcas, AlejandraGarate Ormaechea, AgustínLópez Rayo, SandraLucena Marotta, Juan José57 FechlorosisFe chelate reductaseFe uptakeiron deficiencyStrategy IQuímicaUnder iron (Fe)-limited conditions, plants have developed strategies for acquiring this essential micronutrient. Several Fe sources have been studied as potential fertilizers, with Fe synthetic chelates being the most used to prevent and correct Fe chlorosis in crops. The determination of the activity of the Fe chelate reductase (FCR) enzyme has long been described in the literature to understand the efficiency of Strategy I plants in acquiring Fe from fertilizers under deficient conditions. Other experiments have focused on the translocation of Fe to the plant to define the effectiveness of Fe fertilizers. Yet, both assays are relevant in knowing the capacity of a novel Fe source and other compounds alleviating Fe chlorosis in Strategy I plants. This work reviews the methodologies that are used in FCR assays to evaluate novel Fe fertilizers, including the factors modulating the results obtained for FCR assay activity, such as the Fe substrate, the Fe level during the growing period and during the FCR assay, the pH, the choice of an in vivo or in vitro method, and the plant species. A discussion of the benefits of the concurrence of FCR and Fe uptake assays is then presented alongside a proposed methodology for assessing the effectiveness of Fe fertilizers, emphasizing the importance of understanding chemical and physiological plant interactions. This methodology unifies key factors that modify FCR activity and combines these with the use of the 57Fe tracer to enhance our comprehension of the efficacy of Fe-based fertilizers’ effectiveness in alleviating Fe chlorosis. This comprehensive approach not only contributes to the fundamental understanding of Fe-deficient Strategy I plants but also establishes a robust method for determining the efficiency of novel sources for correcting Fe deficiency in plantsAuthors gratefully acknowledge the financial support the Grant PID2022-141721OB-C2 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”, by the “European Union”MDPIDepartamento de Química Agrícola y BromatologíaFacultad de Ciencias20242024-03-13research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/715237https://dx.doi.org/10.3390/plants13060819reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7152372026-06-23T12:46:27Z
dc.title.none.fl_str_mv A critical review of methodologies for evaluating iron fertilizers based on iron reduction and uptake by strategy I plants
title A critical review of methodologies for evaluating iron fertilizers based on iron reduction and uptake by strategy I plants
spellingShingle A critical review of methodologies for evaluating iron fertilizers based on iron reduction and uptake by strategy I plants
Arcas, Alejandra
57 Fe
chlorosis
Fe chelate reductase
Fe uptake
iron deficiency
Strategy I
Química
title_short A critical review of methodologies for evaluating iron fertilizers based on iron reduction and uptake by strategy I plants
title_full A critical review of methodologies for evaluating iron fertilizers based on iron reduction and uptake by strategy I plants
title_fullStr A critical review of methodologies for evaluating iron fertilizers based on iron reduction and uptake by strategy I plants
title_full_unstemmed A critical review of methodologies for evaluating iron fertilizers based on iron reduction and uptake by strategy I plants
title_sort A critical review of methodologies for evaluating iron fertilizers based on iron reduction and uptake by strategy I plants
dc.creator.none.fl_str_mv Arcas, Alejandra
Garate Ormaechea, Agustín
López Rayo, Sandra
Lucena Marotta, Juan José
author Arcas, Alejandra
author_facet Arcas, Alejandra
Garate Ormaechea, Agustín
López Rayo, Sandra
Lucena Marotta, Juan José
author_role author
author2 Garate Ormaechea, Agustín
López Rayo, Sandra
Lucena Marotta, Juan José
author2_role author
author
author
dc.contributor.none.fl_str_mv Departamento de Química Agrícola y Bromatología
Facultad de Ciencias
dc.subject.none.fl_str_mv 57 Fe
chlorosis
Fe chelate reductase
Fe uptake
iron deficiency
Strategy I
Química
topic 57 Fe
chlorosis
Fe chelate reductase
Fe uptake
iron deficiency
Strategy I
Química
description Under iron (Fe)-limited conditions, plants have developed strategies for acquiring this essential micronutrient. Several Fe sources have been studied as potential fertilizers, with Fe synthetic chelates being the most used to prevent and correct Fe chlorosis in crops. The determination of the activity of the Fe chelate reductase (FCR) enzyme has long been described in the literature to understand the efficiency of Strategy I plants in acquiring Fe from fertilizers under deficient conditions. Other experiments have focused on the translocation of Fe to the plant to define the effectiveness of Fe fertilizers. Yet, both assays are relevant in knowing the capacity of a novel Fe source and other compounds alleviating Fe chlorosis in Strategy I plants. This work reviews the methodologies that are used in FCR assays to evaluate novel Fe fertilizers, including the factors modulating the results obtained for FCR assay activity, such as the Fe substrate, the Fe level during the growing period and during the FCR assay, the pH, the choice of an in vivo or in vitro method, and the plant species. A discussion of the benefits of the concurrence of FCR and Fe uptake assays is then presented alongside a proposed methodology for assessing the effectiveness of Fe fertilizers, emphasizing the importance of understanding chemical and physiological plant interactions. This methodology unifies key factors that modify FCR activity and combines these with the use of the 57Fe tracer to enhance our comprehension of the efficacy of Fe-based fertilizers’ effectiveness in alleviating Fe chlorosis. This comprehensive approach not only contributes to the fundamental understanding of Fe-deficient Strategy I plants but also establishes a robust method for determining the efficiency of novel sources for correcting Fe deficiency in plants
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-03-13
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/715237
https://dx.doi.org/10.3390/plants13060819
url http://hdl.handle.net/10486/715237
https://dx.doi.org/10.3390/plants13060819
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419883670798336
score 15.812429

Similar Items