Comparative proteomic analysis of trypomastigotes from Trypanosoma cruzi strains with different pathogenicity

Chagas disease, caused by the parasite Trypanosoma cruzi, is one of the most neglected diseases in Latin America, being currently a global health problem. Its immunopathogenesis is still quite unknown. Moreover, there are important differences in pathogenicity between some different T. cruzi strains...

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Autores: Herreros-Cabello, Alfonso, Callejas-Hernández, Francisco, Fresno, Manuel, Gironès, Núria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/217184
Acceso en línea:http://hdl.handle.net/10261/217184
Access Level:acceso abierto
Palabra clave:Trypanosoma cruzi
Chagas disease
Trypomastigote
Proteome
Gene ontology
Enriched metabolic pathways
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spelling Comparative proteomic analysis of trypomastigotes from Trypanosoma cruzi strains with different pathogenicityHerreros-Cabello, AlfonsoCallejas-Hernández, FranciscoFresno, ManuelGironès, NúriaTrypanosoma cruziChagas diseaseTrypomastigoteProteomeGene ontologyEnriched metabolic pathwaysChagas disease, caused by the parasite Trypanosoma cruzi, is one of the most neglected diseases in Latin America, being currently a global health problem. Its immunopathogenesis is still quite unknown. Moreover, there are important differences in pathogenicity between some different T. cruzi strains. For example, in mice, Y strain produces a high acute lethality while VFRA remains in the host mostly in a chronic manner. Comparative proteomic studies between T. cruzi strains represent a complement for transcriptomics and may allow the detection of relevant factors or distinctive functions. Here for the first time, we compared the proteome of trypomastigotes from 2 strains, Y and VFRA, analyzed by mass spectrometry. Gene ontology analysis were used to display similarities or differences in cellular components, biological processes and molecular functions. Also, we performed metabolic pathways enrichment analysis to detect the most relevant pathways in each strain. Although in general they have similar profiles in the different ontology groups, there were some particular interesting differences. Moreover, there were around 10% of different proteins between Y and VFRA strains, that were shared by other T. cruzi strains or protozoan species. They displayed many common enriched metabolic pathways but some others were uniquely enriched in one strain. Thus, we detected enriched antioxidant defenses in VFRA that could correlate with its ability to induce a chronic infection in mice controlling ROS production, while the Y strain revealed a great enrichment of pathways related with nucleotides and protein production, that could fit with its high parasite replication and lethality. In summary, Y and VFRA strains displayed comparable proteomes with some particular distinctions that could contribute to understand their different biological behaviors.“Ministerio de Economía y competitividad” SAF2015-63868-R (MINECO/FEDER) and PGC2018-096132-BI00 (MICINN/FEDER) to N.G., SAF2016-75988-R (MINECO/FEDER) to M.F.); “Red de Investigación de Centros de Enfermedades Tropicales, Instituto de Salud Carlos III” (RICET RD12/0018/0004 to M.F.); Comunidad de Madrid (S-2010/BMD-2332 to M.F.); and Institutional grants from “Fundación Ramón Areces” and “Banco de Santander"Ministerio de Economía y Competitividad (España)Red de Investigación Cooperativa en Enfermedades Tropicales (España)Comunidad de MadridFundación Ramón ArecesBanco SantanderConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/217184reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.meegid.2019.104041Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2171842026-05-22T06:33:51Z
dc.title.none.fl_str_mv Comparative proteomic analysis of trypomastigotes from Trypanosoma cruzi strains with different pathogenicity
title Comparative proteomic analysis of trypomastigotes from Trypanosoma cruzi strains with different pathogenicity
spellingShingle Comparative proteomic analysis of trypomastigotes from Trypanosoma cruzi strains with different pathogenicity
Herreros-Cabello, Alfonso
Trypanosoma cruzi
Chagas disease
Trypomastigote
Proteome
Gene ontology
Enriched metabolic pathways
title_short Comparative proteomic analysis of trypomastigotes from Trypanosoma cruzi strains with different pathogenicity
title_full Comparative proteomic analysis of trypomastigotes from Trypanosoma cruzi strains with different pathogenicity
title_fullStr Comparative proteomic analysis of trypomastigotes from Trypanosoma cruzi strains with different pathogenicity
title_full_unstemmed Comparative proteomic analysis of trypomastigotes from Trypanosoma cruzi strains with different pathogenicity
title_sort Comparative proteomic analysis of trypomastigotes from Trypanosoma cruzi strains with different pathogenicity
dc.creator.none.fl_str_mv Herreros-Cabello, Alfonso
Callejas-Hernández, Francisco
Fresno, Manuel
Gironès, Núria
author Herreros-Cabello, Alfonso
author_facet Herreros-Cabello, Alfonso
Callejas-Hernández, Francisco
Fresno, Manuel
Gironès, Núria
author_role author
author2 Callejas-Hernández, Francisco
Fresno, Manuel
Gironès, Núria
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Red de Investigación Cooperativa en Enfermedades Tropicales (España)
Comunidad de Madrid
Fundación Ramón Areces
Banco Santander
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Trypanosoma cruzi
Chagas disease
Trypomastigote
Proteome
Gene ontology
Enriched metabolic pathways
topic Trypanosoma cruzi
Chagas disease
Trypomastigote
Proteome
Gene ontology
Enriched metabolic pathways
description Chagas disease, caused by the parasite Trypanosoma cruzi, is one of the most neglected diseases in Latin America, being currently a global health problem. Its immunopathogenesis is still quite unknown. Moreover, there are important differences in pathogenicity between some different T. cruzi strains. For example, in mice, Y strain produces a high acute lethality while VFRA remains in the host mostly in a chronic manner. Comparative proteomic studies between T. cruzi strains represent a complement for transcriptomics and may allow the detection of relevant factors or distinctive functions. Here for the first time, we compared the proteome of trypomastigotes from 2 strains, Y and VFRA, analyzed by mass spectrometry. Gene ontology analysis were used to display similarities or differences in cellular components, biological processes and molecular functions. Also, we performed metabolic pathways enrichment analysis to detect the most relevant pathways in each strain. Although in general they have similar profiles in the different ontology groups, there were some particular interesting differences. Moreover, there were around 10% of different proteins between Y and VFRA strains, that were shared by other T. cruzi strains or protozoan species. They displayed many common enriched metabolic pathways but some others were uniquely enriched in one strain. Thus, we detected enriched antioxidant defenses in VFRA that could correlate with its ability to induce a chronic infection in mice controlling ROS production, while the Y strain revealed a great enrichment of pathways related with nucleotides and protein production, that could fit with its high parasite replication and lethality. In summary, Y and VFRA strains displayed comparable proteomes with some particular distinctions that could contribute to understand their different biological behaviors.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/217184
url http://hdl.handle.net/10261/217184
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.meegid.2019.104041
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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