EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation
Mitochondria are subcellular organelles that are critical for meeting the bioenergetic and biosynthetic needs of the cell. Mitochondrial function relies on genes and RNA species encoded both in the nucleus and mitochondria, and on their coordinated translation, import and respiratory complex assembl...
| Autores: | , , , , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/119822 |
| Acceso en línea: | https://hdl.handle.net/2445/119822 |
| Access Level: | acceso abierto |
| Palabra clave: | Mitocondris Drosòfila melanogaster Homeòstasi Mitochondria Drosophila melanogaster Homeostasis |
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EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translationSilva, JoanaAivio, SuviKnobel, Philip A.Bailey, Laura J.Casali, AndreuVinaixa, MariaGarcia Cao, IsabelCoyaud, ÉtienneJourdain, Alexis A.Pérez Ferreros, PabloRojas, Ana M.Antolin Fontes, AlbertSamino Gené, SaraRaught, BrianGonzález Reyes, AcaimoRibas de Pouplana, LluísDoherty, Aidan J.Yanes, OscarStracker, Travis H.MitocondrisDrosòfila melanogasterHomeòstasiMitochondriaDrosophila melanogasterHomeostasisMitochondria are subcellular organelles that are critical for meeting the bioenergetic and biosynthetic needs of the cell. Mitochondrial function relies on genes and RNA species encoded both in the nucleus and mitochondria, and on their coordinated translation, import and respiratory complex assembly. Here, we characterize EXD2 (exonuclease 3′–5′ domain-containing 2), a nuclear-encoded gene, and show that it is targeted to the mitochondria and prevents the aberrant association of messenger RNAs with the mitochondrial ribosome. Loss of EXD2 results in defective mitochondrial translation, impaired respiration, reduced ATP production, increased reactive oxygen species and widespread metabolic abnormalities. Depletion of the Drosophila melanogaster EXD2 orthologue (CG6744) causes developmental delays and premature female germline stem cell attrition, reduced fecundity and a dramatic extension of lifespan that is reversed with an antioxidant diet. Our results define a conserved role for EXD2 in mitochondrial translation that influences development and ageing.Macmillan2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/119822Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1038/s41556-017-0016-9Nature Cell Biology, 2018, vol. 20, num. 2, p. 162-174http://dx.doi.org/10.1038/s41556-017-0016-9(c) Nature, 2018info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1198222026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation |
| title |
EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation |
| spellingShingle |
EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation Silva, Joana Mitocondris Drosòfila melanogaster Homeòstasi Mitochondria Drosophila melanogaster Homeostasis |
| title_short |
EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation |
| title_full |
EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation |
| title_fullStr |
EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation |
| title_full_unstemmed |
EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation |
| title_sort |
EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation |
| dc.creator.none.fl_str_mv |
Silva, Joana Aivio, Suvi Knobel, Philip A. Bailey, Laura J. Casali, Andreu Vinaixa, Maria Garcia Cao, Isabel Coyaud, Étienne Jourdain, Alexis A. Pérez Ferreros, Pablo Rojas, Ana M. Antolin Fontes, Albert Samino Gené, Sara Raught, Brian González Reyes, Acaimo Ribas de Pouplana, Lluís Doherty, Aidan J. Yanes, Oscar Stracker, Travis H. |
| author |
Silva, Joana |
| author_facet |
Silva, Joana Aivio, Suvi Knobel, Philip A. Bailey, Laura J. Casali, Andreu Vinaixa, Maria Garcia Cao, Isabel Coyaud, Étienne Jourdain, Alexis A. Pérez Ferreros, Pablo Rojas, Ana M. Antolin Fontes, Albert Samino Gené, Sara Raught, Brian González Reyes, Acaimo Ribas de Pouplana, Lluís Doherty, Aidan J. Yanes, Oscar Stracker, Travis H. |
| author_role |
author |
| author2 |
Aivio, Suvi Knobel, Philip A. Bailey, Laura J. Casali, Andreu Vinaixa, Maria Garcia Cao, Isabel Coyaud, Étienne Jourdain, Alexis A. Pérez Ferreros, Pablo Rojas, Ana M. Antolin Fontes, Albert Samino Gené, Sara Raught, Brian González Reyes, Acaimo Ribas de Pouplana, Lluís Doherty, Aidan J. Yanes, Oscar Stracker, Travis H. |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Mitocondris Drosòfila melanogaster Homeòstasi Mitochondria Drosophila melanogaster Homeostasis |
| topic |
Mitocondris Drosòfila melanogaster Homeòstasi Mitochondria Drosophila melanogaster Homeostasis |
| description |
Mitochondria are subcellular organelles that are critical for meeting the bioenergetic and biosynthetic needs of the cell. Mitochondrial function relies on genes and RNA species encoded both in the nucleus and mitochondria, and on their coordinated translation, import and respiratory complex assembly. Here, we characterize EXD2 (exonuclease 3′–5′ domain-containing 2), a nuclear-encoded gene, and show that it is targeted to the mitochondria and prevents the aberrant association of messenger RNAs with the mitochondrial ribosome. Loss of EXD2 results in defective mitochondrial translation, impaired respiration, reduced ATP production, increased reactive oxygen species and widespread metabolic abnormalities. Depletion of the Drosophila melanogaster EXD2 orthologue (CG6744) causes developmental delays and premature female germline stem cell attrition, reduced fecundity and a dramatic extension of lifespan that is reversed with an antioxidant diet. Our results define a conserved role for EXD2 in mitochondrial translation that influences development and ageing. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/119822 |
| url |
https://hdl.handle.net/2445/119822 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: http://dx.doi.org/10.1038/s41556-017-0016-9 Nature Cell Biology, 2018, vol. 20, num. 2, p. 162-174 http://dx.doi.org/10.1038/s41556-017-0016-9 |
| dc.rights.none.fl_str_mv |
(c) Nature, 2018 info:eu-repo/semantics/openAccess |
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(c) Nature, 2018 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Macmillan |
| publisher.none.fl_str_mv |
Macmillan |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
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Universidad de Barcelona |
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Dipòsit Digital de la UB |
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Dipòsit Digital de la UB |
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|
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1869419866241368064 |
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15,300719 |