Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish

[Background] The development of a sustainable business model with social acceptance, makes necessary to develop new strategies to guarantee the growth, health, and well-being of farmed animals. Debaryomyces hansenii is a yeast species that can be used as a probiotic in aquaculture due to its capacit...

Descripción completa

Detalles Bibliográficos
Autores: Sanahuja, Ignasi, Ruiz, Alberto, Firmino, Joana P., Reyes-López, Felipe E., Ortiz-Delgado, Juan B., Vallejos-Vidal, Eva, Tort, Lluis, Tovar-Ramírez, Dariel, Cerezo, Isabel M., Moriñigo, Miguel A., Sarasquete, Carmen, Gisbert, Enric
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/340829
Acceso en línea:http://hdl.handle.net/10261/340829
https://api.elsevier.com/content/abstract/scopus_id/85164352932
Access Level:acceso abierto
Palabra clave:Debaryomyces hansenii
Intestine condition
Low fish meal diet
Microbiota
Transcriptomics
Yeast probiotic
id ES_cd8f6428b074776ec11cb71e91ee045e
oai_identifier_str oai:digital.csic.es:10261/340829
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish
title Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish
spellingShingle Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish
Sanahuja, Ignasi
Debaryomyces hansenii
Intestine condition
Low fish meal diet
Microbiota
Transcriptomics
Yeast probiotic
title_short Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish
title_full Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish
title_fullStr Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish
title_full_unstemmed Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish
title_sort Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish
dc.creator.none.fl_str_mv Sanahuja, Ignasi
Ruiz, Alberto
Firmino, Joana P.
Reyes-López, Felipe E.
Ortiz-Delgado, Juan B.
Vallejos-Vidal, Eva
Tort, Lluis
Tovar-Ramírez, Dariel
Cerezo, Isabel M.
Moriñigo, Miguel A.
Sarasquete, Carmen
Gisbert, Enric
author Sanahuja, Ignasi
author_facet Sanahuja, Ignasi
Ruiz, Alberto
Firmino, Joana P.
Reyes-López, Felipe E.
Ortiz-Delgado, Juan B.
Vallejos-Vidal, Eva
Tort, Lluis
Tovar-Ramírez, Dariel
Cerezo, Isabel M.
Moriñigo, Miguel A.
Sarasquete, Carmen
Gisbert, Enric
author_role author
author2 Ruiz, Alberto
Firmino, Joana P.
Reyes-López, Felipe E.
Ortiz-Delgado, Juan B.
Vallejos-Vidal, Eva
Tort, Lluis
Tovar-Ramírez, Dariel
Cerezo, Isabel M.
Moriñigo, Miguel A.
Sarasquete, Carmen
Gisbert, Enric
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Agricultura, Pesca y Alimentación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)
Agencia Nacional de Investigación y Desarrollo (Chile)
Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo
Gisbert, Enric [0000-0002-7457-8468]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Debaryomyces hansenii
Intestine condition
Low fish meal diet
Microbiota
Transcriptomics
Yeast probiotic
topic Debaryomyces hansenii
Intestine condition
Low fish meal diet
Microbiota
Transcriptomics
Yeast probiotic
description [Background] The development of a sustainable business model with social acceptance, makes necessary to develop new strategies to guarantee the growth, health, and well-being of farmed animals. Debaryomyces hansenii is a yeast species that can be used as a probiotic in aquaculture due to its capacity to i) promote cell proliferation and differentiation, ii) have immunostimulatory effects, iii) modulate gut microbiota, and/or iv) enhance the digestive function. To provide inside into the effects of D. hansenii on juveniles of gilthead seabream (Sparus aurata) condition, we integrated the evaluation of the main key performance indicators coupled with the integrative analysis of the intestine condition, through histological and microbiota state, and its transcriptomic profiling.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/340829
https://api.elsevier.com/content/abstract/scopus_id/85164352932
url http://hdl.handle.net/10261/340829
https://api.elsevier.com/content/abstract/scopus_id/85164352932
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106878RB-I00
info:eu-repo/grantAgreement/AEI//FJC2020-043933-I
Sanahuja, Ignasi; Ruiz, Alberto; Firmino, Joana P.; Reyes-López, Felipe E.; Ortiz-Delgado, Juan B.; Vallejos-Vidal, Eva; Tort, Lluis; Tovar-Ramírez, Dariel; Cerezo, Isabel M.; Moriñigo, Miguel A.; Sarasquete, Carmen; Gisbert, Enric; 2023; Supplementary Information of the article Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish [Dataset]; BioMed Central; https://doi.org/10.1186/s40104-023-00895-4
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1186/s40104-023-00895-4
https://doi.org/10.1186/s40104-023-00895-4
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419865695059968
spelling Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fishSanahuja, IgnasiRuiz, AlbertoFirmino, Joana P.Reyes-López, Felipe E.Ortiz-Delgado, Juan B.Vallejos-Vidal, EvaTort, LluisTovar-Ramírez, DarielCerezo, Isabel M.Moriñigo, Miguel A.Sarasquete, CarmenGisbert, EnricDebaryomyces hanseniiIntestine conditionLow fish meal dietMicrobiotaTranscriptomicsYeast probiotic[Background] The development of a sustainable business model with social acceptance, makes necessary to develop new strategies to guarantee the growth, health, and well-being of farmed animals. Debaryomyces hansenii is a yeast species that can be used as a probiotic in aquaculture due to its capacity to i) promote cell proliferation and differentiation, ii) have immunostimulatory effects, iii) modulate gut microbiota, and/or iv) enhance the digestive function. To provide inside into the effects of D. hansenii on juveniles of gilthead seabream (Sparus aurata) condition, we integrated the evaluation of the main key performance indicators coupled with the integrative analysis of the intestine condition, through histological and microbiota state, and its transcriptomic profiling.[Results] After 70 days of a nutritional trial in which a diet with low levels of fishmeal (7%) was supplemented with 1.1% of D. hansenii (17.2 × 105 CFU), an increase of ca. 12% in somatic growth was observed together with an improvement in feed conversion in fish fed a yeast-supplemented diet. In terms of intestinal condition, this probiotic modulated gut microbiota without affecting the intestine cell organization, whereas an increase in the staining intensity of mucins rich in carboxylated and weakly sulphated glycoconjugates coupled with changes in the affinity for certain lectins were noted in goblet cells. Changes in microbiota were characterized by the reduction in abundance of several groups of Proteobacteria, especially those characterized as opportunistic groups. The microarrays-based transcriptomic analysis found 232 differential expressed genes in the anterior-mid intestine of S. aurata, that were mostly related to metabolic, antioxidant, immune, and symbiotic processes.[Conclusions] Dietary administration of D. hansenii enhanced somatic growth and improved feed efficiency parameters, results that were coupled to an improvement of intestinal condition as histochemical and transcriptomic tools indicated. This probiotic yeast stimulated host-microbiota interactions without altering the intestinal cell organization nor generating dysbiosis, which demonstrated its safety as a feed additive. At the transcriptomic level, D. hansenii promoted metabolic pathways, mainly protein-related, sphingolipid, and thymidylate pathways, in addition to enhance antioxidant-related intestinal mechanisms, and to regulate sentinel immune processes, potentiating the defensive capacity meanwhile maintaining the homeostatic status of the intestine.This work has been financed through the DIETAplus project of JACUMAR (Junta de Cultivos Marinos, MAPAMA; Spanish government), which is co-funded with FEMP funds (EU). Furthermore, this research was funded by means of grants from the Spanish Government: PID2019-106878RB-I00 and IS was granted with a Postdoctoral fellowship (FJC2020-043933-I). EVV and FERL thank the support of Fondecyt iniciación (project number 11221308) and Fondecyt regular (project number 11221308) grants (Agencia Nacional de Investigacióny Desarrollo de Chile, Government of Chile), respectively. Collaboration between Ibero-American researchers has been done under the framework of the network LARVAplus “Strategies for the development and im-provement of fish larvae production in Ibero-America” (117RT0521) funded by the Ibero-American Program of Science and Technology for Development (CYTED, Spain).Peer reviewedBioMed CentralMinisterio de Agricultura, Pesca y Alimentación (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)Agencia Nacional de Investigación y Desarrollo (Chile)Programa Iberoamericano de Ciencia y Tecnología para el DesarrolloGisbert, Enric [0000-0002-7457-8468]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/340829https://api.elsevier.com/content/abstract/scopus_id/85164352932reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106878RB-I00info:eu-repo/grantAgreement/AEI//FJC2020-043933-ISanahuja, Ignasi; Ruiz, Alberto; Firmino, Joana P.; Reyes-López, Felipe E.; Ortiz-Delgado, Juan B.; Vallejos-Vidal, Eva; Tort, Lluis; Tovar-Ramírez, Dariel; Cerezo, Isabel M.; Moriñigo, Miguel A.; Sarasquete, Carmen; Gisbert, Enric; 2023; Supplementary Information of the article Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish [Dataset]; BioMed Central; https://doi.org/10.1186/s40104-023-00895-4The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1186/s40104-023-00895-4https://doi.org/10.1186/s40104-023-00895-4Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3408292026-05-22T06:33:51Z
score 15,812429

Similares en LA Referencia