Apaf1 plays a pro-survival role by regulating centrosome morphology and function

14 páginas, 10 figuras. PMID:21984814[PubMed]

Detalles Bibliográficos
Autores: Ferraro, Elisabetta, Pesaresi, Maria Grazia, De Zio, Daniela, Cencioni, Maria Teresa, Gortat, Anna, Cozzolino, Mauro, Berghella, Libera, Salvatore, Anna Maria, Oettinghaus, Bjorn, Scorrano, Luca, Pérez-Payá, Enrique, Cecconi, Francesco
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/48368
Acceso en línea:http://hdl.handle.net/10261/48368
Access Level:acceso abierto
Palabra clave:Apaf-1
Centrosomes
Microtubules
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spelling Apaf1 plays a pro-survival role by regulating centrosome morphology and functionFerraro, ElisabettaPesaresi, Maria GraziaDe Zio, DanielaCencioni, Maria TeresaGortat, AnnaCozzolino, MauroBerghella, LiberaSalvatore, Anna MariaOettinghaus, BjornScorrano, LucaPérez-Payá, EnriqueCecconi, FrancescoApaf-1CentrosomesMicrotubules14 páginas, 10 figuras. PMID:21984814[PubMed]The apoptotic protease activating factor 1 (Apaf1) is the main component of the apoptosome, and a crucial factor in the mitochondria-dependent death pathway. Here we show that Apaf1 plays a role in regulating centrosome maturation. By analyzing Apaf1-depleted cells, we have found that Apaf1 loss induces centrosome defects that impair centrosomal microtubule nucleation and cytoskeleton organization. This, in turn, affects several cellular processes such as mitotic spindle formation, cell migration and mitochondrial network regulation. As a consequence, Apaf1-depleted cells are more fragile and have a lower threshold to stress than wild-type cells. In fact, we found that they exhibit low Bcl-2 and Bcl-X(L) expression and, under apoptotic treatment, rapidly release cytochrome c. We also show that Apaf1 acts by regulating the recruitment of HCA66, with which it interacts, to the centrosome. This function of Apaf1 is carried out during the cell life and is not related to its apoptotic role. Therefore, Apaf1 might also be considered a pro-survival molecule, whose absence impairs cell performance and causes a higher responsiveness to stressful conditionsThis work was supported by grants from the Associazione Italiana per la Ricerca sul Cancro (AIRC) [grant no. IG10081], the Telethon Foundation [grant no. S99038TELC], the Health and the Italian Ministry of University and Research [grant nos RF07.96M2, 020903305]. D. De Zio is a FILAS-Regione Lazio fellow.Peer reviewedCompany of Biologists201220122011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/48368reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1242/jcs.086298info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/483682026-05-22T06:33:51Z
dc.title.none.fl_str_mv Apaf1 plays a pro-survival role by regulating centrosome morphology and function
title Apaf1 plays a pro-survival role by regulating centrosome morphology and function
spellingShingle Apaf1 plays a pro-survival role by regulating centrosome morphology and function
Ferraro, Elisabetta
Apaf-1
Centrosomes
Microtubules
title_short Apaf1 plays a pro-survival role by regulating centrosome morphology and function
title_full Apaf1 plays a pro-survival role by regulating centrosome morphology and function
title_fullStr Apaf1 plays a pro-survival role by regulating centrosome morphology and function
title_full_unstemmed Apaf1 plays a pro-survival role by regulating centrosome morphology and function
title_sort Apaf1 plays a pro-survival role by regulating centrosome morphology and function
dc.creator.none.fl_str_mv Ferraro, Elisabetta
Pesaresi, Maria Grazia
De Zio, Daniela
Cencioni, Maria Teresa
Gortat, Anna
Cozzolino, Mauro
Berghella, Libera
Salvatore, Anna Maria
Oettinghaus, Bjorn
Scorrano, Luca
Pérez-Payá, Enrique
Cecconi, Francesco
author Ferraro, Elisabetta
author_facet Ferraro, Elisabetta
Pesaresi, Maria Grazia
De Zio, Daniela
Cencioni, Maria Teresa
Gortat, Anna
Cozzolino, Mauro
Berghella, Libera
Salvatore, Anna Maria
Oettinghaus, Bjorn
Scorrano, Luca
Pérez-Payá, Enrique
Cecconi, Francesco
author_role author
author2 Pesaresi, Maria Grazia
De Zio, Daniela
Cencioni, Maria Teresa
Gortat, Anna
Cozzolino, Mauro
Berghella, Libera
Salvatore, Anna Maria
Oettinghaus, Bjorn
Scorrano, Luca
Pérez-Payá, Enrique
Cecconi, Francesco
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Apaf-1
Centrosomes
Microtubules
topic Apaf-1
Centrosomes
Microtubules
description 14 páginas, 10 figuras. PMID:21984814[PubMed]
publishDate 2011
dc.date.none.fl_str_mv 2011
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/48368
url http://hdl.handle.net/10261/48368
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1242/jcs.086298
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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