Regulation of decapentaplegic expression during Drosophila wing veins pupal development

The differentiation of veins in the Drosophila wing relies on localised expression of decapentaplegic (dpp) in pro-vein territories during pupal development. The expression of dpp in the pupal veins requires the integrity of the shortvein region (shv), localised 5′ to the coding region. It is likely...

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Detalles Bibliográficos
Autores: Sotillos, Sol, Celis, José F. de
Tipo de recurso: artículo
Fecha de publicación:2006
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/4735
Acceso en línea:http://hdl.handle.net/10261/4735
Access Level:acceso abierto
Palabra clave:Drosophila
Decapentaplegic
Vein differentiation
Gene regulation
Wing
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spelling Regulation of decapentaplegic expression during Drosophila wing veins pupal developmentSotillos, SolCelis, José F. deDrosophilaDecapentaplegicVein differentiationGene regulationWingThe differentiation of veins in the Drosophila wing relies on localised expression of decapentaplegic (dpp) in pro-vein territories during pupal development. The expression of dpp in the pupal veins requires the integrity of the shortvein region (shv), localised 5′ to the coding region. It is likely that this DNA integrates positive and negative regulatory signals directing dpp transcription during pupal development. Here, we identify a minimal 0.9 kb fragment giving localised expression in the vein L5 and a 0.5 kb fragment giving expression in all longitudinal veins. Using a combination of in vivo expression of reporter genes regulated by shv sequences, in vitro binding assays and sequence comparisons between the shv region of different Drosophila species, we found binding sites for the vein-specific transciption factors Araucan, Knirps and Ventral veinless, as well as binding sites for the Dpp pathway effectors Mad and Med. We conclude that conserved vein-specific enhancers regulated by transcription factors expressed in individual veins collaborate with general vein and intervein regulators to establish and maintain the expression of dpp confined to the veins during pupal developmentA postdoctoral short-term fellowship from the European Molecular Biology Organization (EMBO) to S. S. is acknowledged. Grants from Dirección General de Investigación Científica y Técnica to James Castelli-Gair (BFU2004-01069/BMC), to J.F.d C. (BCM2003-1191 and GEN2001-4846-C05-01), as well as an institutional grant from Fundación Ramón Areces to the Centro de Biología Molecular “Severo Ochoa” are also acknowledgedPeer reviewedElsevierEMBOMinisterio de Economía y Competitividad (España)Fundación Ramón Areces200820082006info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501696706 bytesapplication/pdfhttp://hdl.handle.net/10261/4735reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.mod.2005.12.002info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/47352026-05-22T06:33:51Z
dc.title.none.fl_str_mv Regulation of decapentaplegic expression during Drosophila wing veins pupal development
title Regulation of decapentaplegic expression during Drosophila wing veins pupal development
spellingShingle Regulation of decapentaplegic expression during Drosophila wing veins pupal development
Sotillos, Sol
Drosophila
Decapentaplegic
Vein differentiation
Gene regulation
Wing
title_short Regulation of decapentaplegic expression during Drosophila wing veins pupal development
title_full Regulation of decapentaplegic expression during Drosophila wing veins pupal development
title_fullStr Regulation of decapentaplegic expression during Drosophila wing veins pupal development
title_full_unstemmed Regulation of decapentaplegic expression during Drosophila wing veins pupal development
title_sort Regulation of decapentaplegic expression during Drosophila wing veins pupal development
dc.creator.none.fl_str_mv Sotillos, Sol
Celis, José F. de
author Sotillos, Sol
author_facet Sotillos, Sol
Celis, José F. de
author_role author
author2 Celis, José F. de
author2_role author
dc.contributor.none.fl_str_mv EMBO
Ministerio de Economía y Competitividad (España)
Fundación Ramón Areces
dc.subject.none.fl_str_mv Drosophila
Decapentaplegic
Vein differentiation
Gene regulation
Wing
topic Drosophila
Decapentaplegic
Vein differentiation
Gene regulation
Wing
description The differentiation of veins in the Drosophila wing relies on localised expression of decapentaplegic (dpp) in pro-vein territories during pupal development. The expression of dpp in the pupal veins requires the integrity of the shortvein region (shv), localised 5′ to the coding region. It is likely that this DNA integrates positive and negative regulatory signals directing dpp transcription during pupal development. Here, we identify a minimal 0.9 kb fragment giving localised expression in the vein L5 and a 0.5 kb fragment giving expression in all longitudinal veins. Using a combination of in vivo expression of reporter genes regulated by shv sequences, in vitro binding assays and sequence comparisons between the shv region of different Drosophila species, we found binding sites for the vein-specific transciption factors Araucan, Knirps and Ventral veinless, as well as binding sites for the Dpp pathway effectors Mad and Med. We conclude that conserved vein-specific enhancers regulated by transcription factors expressed in individual veins collaborate with general vein and intervein regulators to establish and maintain the expression of dpp confined to the veins during pupal development
publishDate 2006
dc.date.none.fl_str_mv 2006
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/4735
url http://hdl.handle.net/10261/4735
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.mod.2005.12.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 696706 bytes
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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