In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extract

Stilbenes are secondary metabolites of great interest produced by many plant species due to their important bioactive properties. These phytochemicals have become of increasing interest in the wine industry as a natural alternative to sulphur dioxide, which has been associated with human health risk...

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Autores: Medrano Padial, Concepción, Prieto Ortega, Ana Isabel, Puerto Rodríguez, María, Pichardo Sánchez, Silvia
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/128534
Acceso en línea:https://hdl.handle.net/11441/128534
https://doi.org/10.1016/j.fct.2021.112065
Access Level:acceso abierto
Palabra clave:Genotoxicity
Mutagenicity
Stilbene extract
Trans-resveratrol
trans-Ɛ-viniferin
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spelling In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extractMedrano Padial, ConcepciónPrieto Ortega, Ana IsabelPuerto Rodríguez, MaríaPichardo Sánchez, SilviaGenotoxicityMutagenicityStilbene extractTrans-resveratroltrans-Ɛ-viniferinStilbenes are secondary metabolites of great interest produced by many plant species due to their important bioactive properties. These phytochemicals have become of increasing interest in the wine industry as a natural alternative to sulphur dioxide, which has been associated with human health risks. However, there is still little toxicological information on stilbenes and the results thus far have been contradictory. Considering the key role of genotoxicity in risk assessment and the need to offer safe products in the market, the aim of this study was to assess the mutagenic and genotoxic potential of a stilbene extract with 99% purity (ST-99 extract). A complete series of different in vitro tests (Ames test, micronucleus (MN) test, and standard and enzyme-modified comet assays) was performed before its use as a preservative in wines. The ST-99 extract induces a significant increase of binucleated cells with micronuclei only in presence of the metabolic fraction S9 at the highest concentration assayed. Neither the Ames test nor the comet assay revealed the extract's genotoxic potential. Further studies are necessary, including in vivo assays, to ensure consumer safety before it can be used.Ministerio de Economía, Industria y Competitividad and INIA (RTA 2015-00005-C02-02 project)ElsevierNutrición y Bromatología, Toxicología y Medicina LegalMinisterio de Economia, Industria y Competitividad (MINECO). EspañaInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/128534https://doi.org/10.1016/j.fct.2021.112065reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésFood and Chemical Toxicology, 150, 112065.RTA 2015-00005-C02-02https://doi.org/10.1016/j.fct.2021.112065info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1285342026-06-17T12:51:07Z
dc.title.none.fl_str_mv In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extract
title In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extract
spellingShingle In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extract
Medrano Padial, Concepción
Genotoxicity
Mutagenicity
Stilbene extract
Trans-resveratrol
trans-Ɛ-viniferin
title_short In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extract
title_full In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extract
title_fullStr In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extract
title_full_unstemmed In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extract
title_sort In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extract
dc.creator.none.fl_str_mv Medrano Padial, Concepción
Prieto Ortega, Ana Isabel
Puerto Rodríguez, María
Pichardo Sánchez, Silvia
author Medrano Padial, Concepción
author_facet Medrano Padial, Concepción
Prieto Ortega, Ana Isabel
Puerto Rodríguez, María
Pichardo Sánchez, Silvia
author_role author
author2 Prieto Ortega, Ana Isabel
Puerto Rodríguez, María
Pichardo Sánchez, Silvia
author2_role author
author
author
dc.contributor.none.fl_str_mv Nutrición y Bromatología, Toxicología y Medicina Legal
Ministerio de Economia, Industria y Competitividad (MINECO). España
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
dc.subject.none.fl_str_mv Genotoxicity
Mutagenicity
Stilbene extract
Trans-resveratrol
trans-Ɛ-viniferin
topic Genotoxicity
Mutagenicity
Stilbene extract
Trans-resveratrol
trans-Ɛ-viniferin
description Stilbenes are secondary metabolites of great interest produced by many plant species due to their important bioactive properties. These phytochemicals have become of increasing interest in the wine industry as a natural alternative to sulphur dioxide, which has been associated with human health risks. However, there is still little toxicological information on stilbenes and the results thus far have been contradictory. Considering the key role of genotoxicity in risk assessment and the need to offer safe products in the market, the aim of this study was to assess the mutagenic and genotoxic potential of a stilbene extract with 99% purity (ST-99 extract). A complete series of different in vitro tests (Ames test, micronucleus (MN) test, and standard and enzyme-modified comet assays) was performed before its use as a preservative in wines. The ST-99 extract induces a significant increase of binucleated cells with micronuclei only in presence of the metabolic fraction S9 at the highest concentration assayed. Neither the Ames test nor the comet assay revealed the extract's genotoxic potential. Further studies are necessary, including in vivo assays, to ensure consumer safety before it can be used.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/128534
https://doi.org/10.1016/j.fct.2021.112065
url https://hdl.handle.net/11441/128534
https://doi.org/10.1016/j.fct.2021.112065
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Food and Chemical Toxicology, 150, 112065.
RTA 2015-00005-C02-02
https://doi.org/10.1016/j.fct.2021.112065
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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