Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum

Background: Plasmodium falciparum drug resistance surveillance is key to successful disease control and eradication. Contemporary methods that only allow determination of prevalence of resistance are expensive, time consuming and require ethical considerations. A newer method involving Next Generati...

Descripción completa

Detalles Bibliográficos
Autores: Smith Aguasca, Rebecca, Gupta, Himanshu, Uberegui, Estefania, Maquina, Mara, Saute, Francisco, Paaijmans, Krijn P., Mayor Aparicio, Alfredo Gabriel, Huijben, Silvie
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/143463
Acceso en línea:https://hdl.handle.net/2445/143463
Access Level:acceso abierto
Palabra clave:Malària
Mosquits
Malaria
Mosquitoes
id ES_cd7703b95d0ed4bbcd3758d7e0581ded
oai_identifier_str oai:recercat.cat:2445/143463
network_acronym_str ES
network_name_str España
repository_id_str
spelling Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparumSmith Aguasca, RebeccaGupta, HimanshuUberegui, EstefaniaMaquina, MaraSaute, FranciscoPaaijmans, Krijn P.Mayor Aparicio, Alfredo GabrielHuijben, SilvieMalàriaMosquitsMalariaMosquitoesBackground: Plasmodium falciparum drug resistance surveillance is key to successful disease control and eradication. Contemporary methods that only allow determination of prevalence of resistance are expensive, time consuming and require ethical considerations. A newer method involving Next Generation Sequencing (NGS) permits obtaining frequency of resistance while allowing to detect minority variants in mixed infections. Here, NGS was tested for P. falciparum resistance marker detection in mosquito samples as a feasible and suitable alternative for molecular resistance surveillance. Anopheles funestus were collected in southern Mozambique using CDC light traps and manual collec‑ tions. DNA was extracted from either whole mosquito, head-thorax and abdomen separately or pools of fve mosqui‑ toes. These samples were screened for P. falciparum and if positive for k13, pfcrt, pfmdr1, pfdhps and pfdhfr mutations related to anti-malarial drug resistance with Sanger sequencing and NGS. Results: Among the 846 samples screened for P. falciparum, 122 were positive by 18S ssrDNA qPCR with an infection rate of 23.6%. No mutations were observed for k13 and pfcrt72-76 and almost zero for pfmdr86, but quintuple pfdhfr/ pfdhps mutations were near fxation and about half of the isolates contained the pfmdr184F polymorphism. Similar allele frequencies of resistance markers were estimated with NGS in comparison with the prevalence of markers obtained with the gold standard Sanger sequencing. Conclusions: Pooled deep sequencing of P. falciparum isolates extracted from mosquitoes is a promising, efcient and cost-efective method to quantify allele frequencies at population level which allows to detect known and unknown markers of resistance in single and mixed infections in a timelier manner. Using mosquitoes as sentinel group and focusing on allele frequency opposed to prevalence, permits active surveillance across a more homogene‑ ous geographical range.BioMed Central2019201920192019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 p.application/pdfhttps://hdl.handle.net/2445/143463Articles publicats en revistes (ISGlobal)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1186/s12936-019-2946-0Malaria Journal, 2019, vol. 18http://dx.doi.org/10.1186/s12936-019-2946-0cc by (c) Smith Aguasca et al., 2019http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1434632026-05-29T05:05:01Z
dc.title.none.fl_str_mv Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum
title Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum
spellingShingle Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum
Smith Aguasca, Rebecca
Malària
Mosquits
Malaria
Mosquitoes
title_short Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum
title_full Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum
title_fullStr Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum
title_full_unstemmed Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum
title_sort Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum
dc.creator.none.fl_str_mv Smith Aguasca, Rebecca
Gupta, Himanshu
Uberegui, Estefania
Maquina, Mara
Saute, Francisco
Paaijmans, Krijn P.
Mayor Aparicio, Alfredo Gabriel
Huijben, Silvie
author Smith Aguasca, Rebecca
author_facet Smith Aguasca, Rebecca
Gupta, Himanshu
Uberegui, Estefania
Maquina, Mara
Saute, Francisco
Paaijmans, Krijn P.
Mayor Aparicio, Alfredo Gabriel
Huijben, Silvie
author_role author
author2 Gupta, Himanshu
Uberegui, Estefania
Maquina, Mara
Saute, Francisco
Paaijmans, Krijn P.
Mayor Aparicio, Alfredo Gabriel
Huijben, Silvie
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Malària
Mosquits
Malaria
Mosquitoes
topic Malària
Mosquits
Malaria
Mosquitoes
description Background: Plasmodium falciparum drug resistance surveillance is key to successful disease control and eradication. Contemporary methods that only allow determination of prevalence of resistance are expensive, time consuming and require ethical considerations. A newer method involving Next Generation Sequencing (NGS) permits obtaining frequency of resistance while allowing to detect minority variants in mixed infections. Here, NGS was tested for P. falciparum resistance marker detection in mosquito samples as a feasible and suitable alternative for molecular resistance surveillance. Anopheles funestus were collected in southern Mozambique using CDC light traps and manual collec‑ tions. DNA was extracted from either whole mosquito, head-thorax and abdomen separately or pools of fve mosqui‑ toes. These samples were screened for P. falciparum and if positive for k13, pfcrt, pfmdr1, pfdhps and pfdhfr mutations related to anti-malarial drug resistance with Sanger sequencing and NGS. Results: Among the 846 samples screened for P. falciparum, 122 were positive by 18S ssrDNA qPCR with an infection rate of 23.6%. No mutations were observed for k13 and pfcrt72-76 and almost zero for pfmdr86, but quintuple pfdhfr/ pfdhps mutations were near fxation and about half of the isolates contained the pfmdr184F polymorphism. Similar allele frequencies of resistance markers were estimated with NGS in comparison with the prevalence of markers obtained with the gold standard Sanger sequencing. Conclusions: Pooled deep sequencing of P. falciparum isolates extracted from mosquitoes is a promising, efcient and cost-efective method to quantify allele frequencies at population level which allows to detect known and unknown markers of resistance in single and mixed infections in a timelier manner. Using mosquitoes as sentinel group and focusing on allele frequency opposed to prevalence, permits active surveillance across a more homogene‑ ous geographical range.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/143463
url https://hdl.handle.net/2445/143463
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1186/s12936-019-2946-0
Malaria Journal, 2019, vol. 18
http://dx.doi.org/10.1186/s12936-019-2946-0
dc.rights.none.fl_str_mv cc by (c) Smith Aguasca et al., 2019
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Smith Aguasca et al., 2019
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (ISGlobal)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419857006559232
score 15,81155

Similares en LA Referencia