Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro models

Soybeans are one of the most used alternative dietary ingredients in aquafeeds. However, they contain phytoestrogens like genistein (GE), which can have an impact on fish metabolism and health. This study aimed to investigate the in vitro and in vivo effects of GE on lipid metabolism, apoptosis, and...

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Autores: Balbuena-Pecino, Sara, Lutfi Royo, Esmail, Riera Heredia, Natàlia, Gasch-Navalón, Esther, Vélez, Emilio J., Gutiérrez Fruitós, Joaquín, Capilla Campos, Encarnación, Navarro Álvarez, Isabel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/173708
Acceso en línea:https://hdl.handle.net/2445/173708
Access Level:acceso abierto
Palabra clave:Truites (Peixos)
Teixit adipós
Trout
Adipose tissues
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spelling Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro modelsBalbuena-Pecino, SaraLutfi Royo, EsmailRiera Heredia, NatàliaGasch-Navalón, EstherVélez, Emilio J.Gutiérrez Fruitós, JoaquínCapilla Campos, EncarnaciónNavarro Álvarez, IsabelTruites (Peixos)Teixit adipósTroutAdipose tissuesSoybeans are one of the most used alternative dietary ingredients in aquafeeds. However, they contain phytoestrogens like genistein (GE), which can have an impact on fish metabolism and health. This study aimed to investigate the in vitro and in vivo effects of GE on lipid metabolism, apoptosis, and autophagy in rainbow trout (Oncorhynchus mykiss). Primary cultured preadipocytes were incubated with GE at different concentrations, 10 or 100 μM, and 1 μM 17β-estradiol (E2). Furthermore, juveniles received an intraperitoneal injection of GE at 5 or 50 µg/g body weight, or E2 at 5 µg/g. In vitro, GE 100 μM increased lipid accumulation and reduced cell viability, apparently involving an autophagic process, indicated by the higher LC3-II protein levels, and higher lc3b and cathepsin d transcript levels achieved after GE 10 μM. In vivo, GE 50 µg/g upregulated the gene expression of fatty acid synthase (fas) and glyceraldehyde-3-phosphate dehydrogenase in adipose tissue, suggesting enhanced lipogenesis, whereas it increased hormone-sensitive lipase in liver, indicating a lipolytic response. Besides, autophagy-related genes increased in the tissues analyzed mainly after GE 50 µg/g treatment. Overall, these findings suggest that an elevated GE administration could lead to impaired adipocyte viability and lipid metabolism dysregulation in rainbow troutMDPI2021202120202021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion24 p.application/pdfhttps://hdl.handle.net/2445/173708Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/ijms21165884International Journal of Molecular Sciences, 2020, vol. 21, num. 16, p. 5884https://doi.org/10.3390/ijms21165884cc-by (c) Balbuena-Pecino, Sara et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1737082026-05-29T05:05:01Z
dc.title.none.fl_str_mv Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro models
title Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro models
spellingShingle Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro models
Balbuena-Pecino, Sara
Truites (Peixos)
Teixit adipós
Trout
Adipose tissues
title_short Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro models
title_full Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro models
title_fullStr Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro models
title_full_unstemmed Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro models
title_sort Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro models
dc.creator.none.fl_str_mv Balbuena-Pecino, Sara
Lutfi Royo, Esmail
Riera Heredia, Natàlia
Gasch-Navalón, Esther
Vélez, Emilio J.
Gutiérrez Fruitós, Joaquín
Capilla Campos, Encarnación
Navarro Álvarez, Isabel
author Balbuena-Pecino, Sara
author_facet Balbuena-Pecino, Sara
Lutfi Royo, Esmail
Riera Heredia, Natàlia
Gasch-Navalón, Esther
Vélez, Emilio J.
Gutiérrez Fruitós, Joaquín
Capilla Campos, Encarnación
Navarro Álvarez, Isabel
author_role author
author2 Lutfi Royo, Esmail
Riera Heredia, Natàlia
Gasch-Navalón, Esther
Vélez, Emilio J.
Gutiérrez Fruitós, Joaquín
Capilla Campos, Encarnación
Navarro Álvarez, Isabel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Truites (Peixos)
Teixit adipós
Trout
Adipose tissues
topic Truites (Peixos)
Teixit adipós
Trout
Adipose tissues
description Soybeans are one of the most used alternative dietary ingredients in aquafeeds. However, they contain phytoestrogens like genistein (GE), which can have an impact on fish metabolism and health. This study aimed to investigate the in vitro and in vivo effects of GE on lipid metabolism, apoptosis, and autophagy in rainbow trout (Oncorhynchus mykiss). Primary cultured preadipocytes were incubated with GE at different concentrations, 10 or 100 μM, and 1 μM 17β-estradiol (E2). Furthermore, juveniles received an intraperitoneal injection of GE at 5 or 50 µg/g body weight, or E2 at 5 µg/g. In vitro, GE 100 μM increased lipid accumulation and reduced cell viability, apparently involving an autophagic process, indicated by the higher LC3-II protein levels, and higher lc3b and cathepsin d transcript levels achieved after GE 10 μM. In vivo, GE 50 µg/g upregulated the gene expression of fatty acid synthase (fas) and glyceraldehyde-3-phosphate dehydrogenase in adipose tissue, suggesting enhanced lipogenesis, whereas it increased hormone-sensitive lipase in liver, indicating a lipolytic response. Besides, autophagy-related genes increased in the tissues analyzed mainly after GE 50 µg/g treatment. Overall, these findings suggest that an elevated GE administration could lead to impaired adipocyte viability and lipid metabolism dysregulation in rainbow trout
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/173708
url https://hdl.handle.net/2445/173708
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/ijms21165884
International Journal of Molecular Sciences, 2020, vol. 21, num. 16, p. 5884
https://doi.org/10.3390/ijms21165884
dc.rights.none.fl_str_mv cc-by (c) Balbuena-Pecino, Sara et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Balbuena-Pecino, Sara et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 24 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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