Lipid alterations in lipid rafts from alzheimer's disease human brain cortex

Lipid rafts are membrane microdomains intimately associated with cell signaling. These biochemical microstructures are characterized by their high contents of sphingolipids, cholesterol and saturated fatty acids and a reduced content of polyunsaturated fatty acids (PUFA). Here, we have purified lipi...

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Detalles Bibliográficos
Autores: Martín, Virginia, Fabelo, Noemí, Santpere Baró, Gabriel, Puig Martorell, Berta, Marín, Raquel, Ferrer, Isidro (Ferrer Abizanda), Díaz, Mario
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/154913
Acceso en línea:https://hdl.handle.net/2445/154913
Access Level:acceso abierto
Palabra clave:Lípids
Escorça cerebral
Malaltia d'Alzheimer
Lipids
Cerebral cortex
Alzheimer's disease
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spelling Lipid alterations in lipid rafts from alzheimer's disease human brain cortexMartín, VirginiaFabelo, NoemíSantpere Baró, GabrielPuig Martorell, BertaMarín, RaquelFerrer, Isidro (Ferrer Abizanda)Díaz, MarioLípidsEscorça cerebralMalaltia d'AlzheimerLipidsCerebral cortexAlzheimer's diseaseLipid rafts are membrane microdomains intimately associated with cell signaling. These biochemical microstructures are characterized by their high contents of sphingolipids, cholesterol and saturated fatty acids and a reduced content of polyunsaturated fatty acids (PUFA). Here, we have purified lipid rafts of human frontal brain cortex from normal and Alzheimer's disease (AD) and characterized their biochemical lipid composition. The results revealed that lipid rafts from AD brains exhibit aberrant lipid profiles compared to healthy brains. In particular, lipid rafts from AD brains displayed abnormally low levels of n-3 long chain polyunsaturated fatty acids (LCPUFA, mainly 22:6n-3, docosahexaenoic acid) and monoenes (mainly 18:1n-9, oleic acid), as well as reduced unsaturation and peroxidability indexes. Also, multiple relationships between phospholipids and fatty acids were altered in AD lipid rafts. Importantly, no changes were observed in the mole percentage of lipid classes and fatty acids in rafts from normal brains throughout the lifespan (24-85 years). These indications point to the existence of homeostatic mechanisms preserving lipid raft status in normal frontal cortex. The disruption of such mechanisms in AD brains leads to a considerable increase in lipid raft order and viscosity, which may explain the alterations in lipid raft signaling observed in AD.IOS Press2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/154913Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3233/JAD-2010-1242Journal of Alzheimer's Disease, 2010, vol. 19, num. 2, p. 489-502https://doi.org/10.3233/JAD-2010-1242(c) Martín, Virginia et al., 2010info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1549132026-05-27T06:46:51Z
dc.title.none.fl_str_mv Lipid alterations in lipid rafts from alzheimer's disease human brain cortex
title Lipid alterations in lipid rafts from alzheimer's disease human brain cortex
spellingShingle Lipid alterations in lipid rafts from alzheimer's disease human brain cortex
Martín, Virginia
Lípids
Escorça cerebral
Malaltia d'Alzheimer
Lipids
Cerebral cortex
Alzheimer's disease
title_short Lipid alterations in lipid rafts from alzheimer's disease human brain cortex
title_full Lipid alterations in lipid rafts from alzheimer's disease human brain cortex
title_fullStr Lipid alterations in lipid rafts from alzheimer's disease human brain cortex
title_full_unstemmed Lipid alterations in lipid rafts from alzheimer's disease human brain cortex
title_sort Lipid alterations in lipid rafts from alzheimer's disease human brain cortex
dc.creator.none.fl_str_mv Martín, Virginia
Fabelo, Noemí
Santpere Baró, Gabriel
Puig Martorell, Berta
Marín, Raquel
Ferrer, Isidro (Ferrer Abizanda)
Díaz, Mario
author Martín, Virginia
author_facet Martín, Virginia
Fabelo, Noemí
Santpere Baró, Gabriel
Puig Martorell, Berta
Marín, Raquel
Ferrer, Isidro (Ferrer Abizanda)
Díaz, Mario
author_role author
author2 Fabelo, Noemí
Santpere Baró, Gabriel
Puig Martorell, Berta
Marín, Raquel
Ferrer, Isidro (Ferrer Abizanda)
Díaz, Mario
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Lípids
Escorça cerebral
Malaltia d'Alzheimer
Lipids
Cerebral cortex
Alzheimer's disease
topic Lípids
Escorça cerebral
Malaltia d'Alzheimer
Lipids
Cerebral cortex
Alzheimer's disease
description Lipid rafts are membrane microdomains intimately associated with cell signaling. These biochemical microstructures are characterized by their high contents of sphingolipids, cholesterol and saturated fatty acids and a reduced content of polyunsaturated fatty acids (PUFA). Here, we have purified lipid rafts of human frontal brain cortex from normal and Alzheimer's disease (AD) and characterized their biochemical lipid composition. The results revealed that lipid rafts from AD brains exhibit aberrant lipid profiles compared to healthy brains. In particular, lipid rafts from AD brains displayed abnormally low levels of n-3 long chain polyunsaturated fatty acids (LCPUFA, mainly 22:6n-3, docosahexaenoic acid) and monoenes (mainly 18:1n-9, oleic acid), as well as reduced unsaturation and peroxidability indexes. Also, multiple relationships between phospholipids and fatty acids were altered in AD lipid rafts. Importantly, no changes were observed in the mole percentage of lipid classes and fatty acids in rafts from normal brains throughout the lifespan (24-85 years). These indications point to the existence of homeostatic mechanisms preserving lipid raft status in normal frontal cortex. The disruption of such mechanisms in AD brains leads to a considerable increase in lipid raft order and viscosity, which may explain the alterations in lipid raft signaling observed in AD.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/154913
url https://hdl.handle.net/2445/154913
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3233/JAD-2010-1242
Journal of Alzheimer's Disease, 2010, vol. 19, num. 2, p. 489-502
https://doi.org/10.3233/JAD-2010-1242
dc.rights.none.fl_str_mv (c) Martín, Virginia et al., 2010
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Martín, Virginia et al., 2010
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv IOS Press
publisher.none.fl_str_mv IOS Press
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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