Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis

Despite the availability of highly sensitive polymerase chain reaction (PCR)-based methods, the dearth of remotely deployable diagnostic tools circumvents the early and accurate detection of individuals with post-kala-azar dermal leishmaniasis (PKDL). Here, we evaluate a design-locked loop-mediated...

Descripción completa

Detalles Bibliográficos
Autores: Ghosh, Prakash, Chowdhury, Rajashree, Maruf, Shomik, Picado, Albert, Hossain, Faria, Owen, Sophie I, Nath, Rupen, Baker, James, Hasnain, Md Golam, Shomik, Mohammad Sohel, Ghosh, Debashis, Rashid, Masud, Rashid, Md Utba, Sagar, Soumik Kha, Rahat, Md Abu, Basher, Ariful, Nath, Proggananda, Edwards, Thomas, Andrews, Jason R, Duthie, Malcolm S, de Souza, Dziedzom K, Adams, Emily R, Ndung'u, Joseph Mathu, Cruz, Israel, Mondal, Dinesh
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/15944
Acceso en línea:http://hdl.handle.net/20.500.12105/15944
Access Level:acceso abierto
Palabra clave:Leishmaniasis, Visceral
Leishmania donovani
Parasites
Leishmaniasis, Cutaneous
Skin Diseases, Parasitic
Animals
Humans
Bayes Theorem
Real-Time Polymerase Chain Reaction
id ES_cd476d44bba8efee6636f4ea33ebb0f6
oai_identifier_str oai:repisalud.isciii.es:20.500.12105/15944
network_acronym_str ES
network_name_str España
repository_id_str
spelling Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasisGhosh, PrakashChowdhury, RajashreeMaruf, ShomikPicado, AlbertHossain, FariaOwen, Sophie INath, RupenBaker, JamesHasnain, Md GolamShomik, Mohammad SohelGhosh, DebashisRashid, MasudRashid, Md UtbaSagar, Soumik KhaRahat, Md AbuBasher, ArifulNath, ProgganandaEdwards, ThomasAndrews, Jason RDuthie, Malcolm Sde Souza, Dziedzom KAdams, Emily RNdung'u, Joseph MathuCruz, IsraelMondal, DineshLeishmaniasis, VisceralLeishmania donovaniParasitesLeishmaniasis, CutaneousSkin Diseases, ParasiticAnimalsHumansBayes TheoremReal-Time Polymerase Chain ReactionDespite the availability of highly sensitive polymerase chain reaction (PCR)-based methods, the dearth of remotely deployable diagnostic tools circumvents the early and accurate detection of individuals with post-kala-azar dermal leishmaniasis (PKDL). Here, we evaluate a design-locked loop-mediated isothermal amplification (LAMP) assay to diagnose PKDL. A total of 76 snip-skin samples collected from individuals with probable PKDL (clinical presentation and a positive rK39 rapid diagnostic test (RDT)) were assessed by microscopy, qPCR, and LAMP. An equal number of age and sex-matched healthy controls were included to determine the specificity of the LAMP assay. The LAMP assay with a Qiagen DNA extraction (Q-LAMP) showed a promising sensitivity of 72.37% (95% CI: 60.91-82.01%) for identifying the PKDL cases. LAMP assay sensitivity declined when the DNA was extracted using a boil-spin method. Q-qPCR showed 68.42% (56.75-78.61%) sensitivity, comparable to LAMP and with an excellent agreement, whereas the microscopy exhibited a weak sensitivity of 39.47% (28.44-51.35%). When microscopy and/or qPCR were considered the gold standard, Q-LAMP exhibited an elevated sensitivity of 89.7% (95% CI: 78.83-96.11%) for detection of PKDL cases and Bayesian latent class modeling substantiated the excellent sensitivity of the assay. All healthy controls were found to be negative. Notwithstanding the optimum efficiency of the LAMP assay towards the detection of PKDL cases, further optimization of the boil-spin method is warranted to permit remote use of the assay.Nature Publishing GroupFederal Ministry of Education & Research (Alemania)Wellcome TrustGovernment of the United KingdomGovernment of NetherlandsGovernment of Switzerland20232023-05-0320222022-10-2720222022-10-27research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/15944reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/159442026-06-12T12:43:37Z
dc.title.none.fl_str_mv Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis
title Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis
spellingShingle Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis
Ghosh, Prakash
Leishmaniasis, Visceral
Leishmania donovani
Parasites
Leishmaniasis, Cutaneous
Skin Diseases, Parasitic
Animals
Humans
Bayes Theorem
Real-Time Polymerase Chain Reaction
title_short Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis
title_full Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis
title_fullStr Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis
title_full_unstemmed Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis
title_sort Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis
dc.creator.none.fl_str_mv Ghosh, Prakash
Chowdhury, Rajashree
Maruf, Shomik
Picado, Albert
Hossain, Faria
Owen, Sophie I
Nath, Rupen
Baker, James
Hasnain, Md Golam
Shomik, Mohammad Sohel
Ghosh, Debashis
Rashid, Masud
Rashid, Md Utba
Sagar, Soumik Kha
Rahat, Md Abu
Basher, Ariful
Nath, Proggananda
Edwards, Thomas
Andrews, Jason R
Duthie, Malcolm S
de Souza, Dziedzom K
Adams, Emily R
Ndung'u, Joseph Mathu
Cruz, Israel
Mondal, Dinesh
author Ghosh, Prakash
author_facet Ghosh, Prakash
Chowdhury, Rajashree
Maruf, Shomik
Picado, Albert
Hossain, Faria
Owen, Sophie I
Nath, Rupen
Baker, James
Hasnain, Md Golam
Shomik, Mohammad Sohel
Ghosh, Debashis
Rashid, Masud
Rashid, Md Utba
Sagar, Soumik Kha
Rahat, Md Abu
Basher, Ariful
Nath, Proggananda
Edwards, Thomas
Andrews, Jason R
Duthie, Malcolm S
de Souza, Dziedzom K
Adams, Emily R
Ndung'u, Joseph Mathu
Cruz, Israel
Mondal, Dinesh
author_role author
author2 Chowdhury, Rajashree
Maruf, Shomik
Picado, Albert
Hossain, Faria
Owen, Sophie I
Nath, Rupen
Baker, James
Hasnain, Md Golam
Shomik, Mohammad Sohel
Ghosh, Debashis
Rashid, Masud
Rashid, Md Utba
Sagar, Soumik Kha
Rahat, Md Abu
Basher, Ariful
Nath, Proggananda
Edwards, Thomas
Andrews, Jason R
Duthie, Malcolm S
de Souza, Dziedzom K
Adams, Emily R
Ndung'u, Joseph Mathu
Cruz, Israel
Mondal, Dinesh
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal Ministry of Education & Research (Alemania)
Wellcome Trust
Government of the United Kingdom
Government of Netherlands
Government of Switzerland
dc.subject.none.fl_str_mv Leishmaniasis, Visceral
Leishmania donovani
Parasites
Leishmaniasis, Cutaneous
Skin Diseases, Parasitic
Animals
Humans
Bayes Theorem
Real-Time Polymerase Chain Reaction
topic Leishmaniasis, Visceral
Leishmania donovani
Parasites
Leishmaniasis, Cutaneous
Skin Diseases, Parasitic
Animals
Humans
Bayes Theorem
Real-Time Polymerase Chain Reaction
description Despite the availability of highly sensitive polymerase chain reaction (PCR)-based methods, the dearth of remotely deployable diagnostic tools circumvents the early and accurate detection of individuals with post-kala-azar dermal leishmaniasis (PKDL). Here, we evaluate a design-locked loop-mediated isothermal amplification (LAMP) assay to diagnose PKDL. A total of 76 snip-skin samples collected from individuals with probable PKDL (clinical presentation and a positive rK39 rapid diagnostic test (RDT)) were assessed by microscopy, qPCR, and LAMP. An equal number of age and sex-matched healthy controls were included to determine the specificity of the LAMP assay. The LAMP assay with a Qiagen DNA extraction (Q-LAMP) showed a promising sensitivity of 72.37% (95% CI: 60.91-82.01%) for identifying the PKDL cases. LAMP assay sensitivity declined when the DNA was extracted using a boil-spin method. Q-qPCR showed 68.42% (56.75-78.61%) sensitivity, comparable to LAMP and with an excellent agreement, whereas the microscopy exhibited a weak sensitivity of 39.47% (28.44-51.35%). When microscopy and/or qPCR were considered the gold standard, Q-LAMP exhibited an elevated sensitivity of 89.7% (95% CI: 78.83-96.11%) for detection of PKDL cases and Bayesian latent class modeling substantiated the excellent sensitivity of the assay. All healthy controls were found to be negative. Notwithstanding the optimum efficiency of the LAMP assay towards the detection of PKDL cases, further optimization of the boil-spin method is warranted to permit remote use of the assay.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-10-27
2022
2022-10-27
2023
2023-05-03
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/15944
url http://hdl.handle.net/20.500.12105/15944
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419795959513088
score 15,81155

Similares en LA Referencia