Bacterial membrane vesicles of Pseudomonas aeruginosa activate adenosine monophosphate-activated protein kinase signaling through inhibition of mitochondrial complex III

Bacterial membrane vesicles (BMVs) are secreted by many pathogenic bacteria and known to stimulate various host responses upon infection, thereby contributing to the pathogenicity of bacterial pathogens like Pseudomonas aeruginosa. While the effects of BMVs on host immune responses are well studied,...

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Detalles Bibliográficos
Autores: Müller, Julia, Kretschmer, Marcel, Opitsch, Elise, Holland, Svea, Borrero de Acuña, José Manuel, Jahn, Dieter, Wegner, Andre
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/179623
Acceso en línea:https://hdl.handle.net/11441/179623
https://doi.org/10.1093/pnasnexus/pgaf248
Access Level:acceso abierto
Palabra clave:Bacterial membrane vesicles (BMVs)
Pseudomonas aeruginosa
Metabolism
Electron transport chain
AMPK
Descripción
Sumario:Bacterial membrane vesicles (BMVs) are secreted by many pathogenic bacteria and known to stimulate various host responses upon infection, thereby contributing to the pathogenicity of bacterial pathogens like Pseudomonas aeruginosa. While the effects of BMVs on host immune responses are well studied, little is known about their impact on cell metabolism and mitochondrial respiration. Here, we show that P. aeruginosa BMVs (i) reprogram cell metabolism of human lung cells, (ii) negatively affect mitochondrial respiration by (iii) specifically inhibiting complex III of the electron transport chain, leading to (iv) the activation of adenosine monophosphate-activated protein kinase (AMPK) signaling, which in turn results in (v) AMPK-dependent inhibition of global protein synthesis.