Evaluating signs of hippocampal neurotoxicity induced by a revisited paradigm of voluntary ethanol consumption in adult male and female Sprague-Dawley rats

Binge alcohol drinking is considered a prominent risk factor for the development of alcohol-use disorders, and could be model in rodents through the standard two-bottle preference choice test. The goal was to recreate an intermittent use of alcohol during 3 consecutive days each week to ascertain it...

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Detalhes bibliográficos
Autores: Colom-Rocha, Carles, Bis-Humbert, Cristian, García-Fuster, M Julia
Tipo de documento: artigo
Data de publicação:2023
País:España
Recursos:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositório:Docusalut
Idioma:inglês
OAI Identifier:oai:docusalut.com:20.500.13003/18903
Acesso em linha:https://hdl.handle.net/20.500.13003/18903
Access Level:Acceso aberto
Palavra-chave:Male
Alcohol Drinking
Rats
Female
Alcoholism
Animals
Hippocampus
Rats, Sprague-Dawley
Ethanol
Animales
Ratas
Ratas Sprague-Dawley
Etanol
Hipocampo
Femenino
Alcoholismo
Consumo de Bebidas Alcohólicas
Masculino
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repository_id_str
spelling Evaluating signs of hippocampal neurotoxicity induced by a revisited paradigm of voluntary ethanol consumption in adult male and female Sprague-Dawley ratsColom-Rocha, CarlesBis-Humbert, CristianGarcía-Fuster, M JuliaMaleAlcohol DrinkingRatsFemaleAlcoholismAnimalsHippocampusRats, Sprague-DawleyEthanolAnimalesRatasRatas Sprague-DawleyEtanolHipocampoFemeninoAlcoholismoConsumo de Bebidas AlcohólicasMasculinoBinge alcohol drinking is considered a prominent risk factor for the development of alcohol-use disorders, and could be model in rodents through the standard two-bottle preference choice test. The goal was to recreate an intermittent use of alcohol during 3 consecutive days each week to ascertain its potential impact on hippocampal neurotoxicity (neurogenesis and other neuroplasticity markers), and including sex as a biological variable, given the well-known sex differences in alcohol consumption. Ethanol access was granted to adult Sprague-Dawley rats for 3 consecutive days per week, followed by 4 days of withdrawal, during 6 weeks, mimicking the most common pattern of intake in people, drinking over the weekends in an intensive manner. Hippocampal samples were collected to evaluate signs of neurotoxicity. Female rats consumed significantly more ethanol than males, although intake did not escalate over time. Ethanol preference levels remained below 40% over time and did not differ between sexes. Moderate signs of ethanol neurotoxicity were observed in hippocampus at the level of decreased neuronal progenitors (NeuroD + cells), and these effects were independent of sex. No other signs of neurotoxicity were induced by ethanol voluntary consumption when measured through several key cell fate markers (i.e., FADD, Cyt c, Cdk5, NF-L) by western blot analysis. Overall, the present results suggest that even though we modeled a situation where no escalation in ethanol intake occurred across time, mild signs of neurotoxicity emerged, suggesting that even the use of ethanol during adulthood in a recreational way could lead to certain brain harm.Springer20232023-04-0120232023-04-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/18903reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/189032026-06-22T12:44:07Z
dc.title.none.fl_str_mv Evaluating signs of hippocampal neurotoxicity induced by a revisited paradigm of voluntary ethanol consumption in adult male and female Sprague-Dawley rats
title Evaluating signs of hippocampal neurotoxicity induced by a revisited paradigm of voluntary ethanol consumption in adult male and female Sprague-Dawley rats
spellingShingle Evaluating signs of hippocampal neurotoxicity induced by a revisited paradigm of voluntary ethanol consumption in adult male and female Sprague-Dawley rats
Colom-Rocha, Carles
Male
Alcohol Drinking
Rats
Female
Alcoholism
Animals
Hippocampus
Rats, Sprague-Dawley
Ethanol
Animales
Ratas
Ratas Sprague-Dawley
Etanol
Hipocampo
Femenino
Alcoholismo
Consumo de Bebidas Alcohólicas
Masculino
title_short Evaluating signs of hippocampal neurotoxicity induced by a revisited paradigm of voluntary ethanol consumption in adult male and female Sprague-Dawley rats
title_full Evaluating signs of hippocampal neurotoxicity induced by a revisited paradigm of voluntary ethanol consumption in adult male and female Sprague-Dawley rats
title_fullStr Evaluating signs of hippocampal neurotoxicity induced by a revisited paradigm of voluntary ethanol consumption in adult male and female Sprague-Dawley rats
title_full_unstemmed Evaluating signs of hippocampal neurotoxicity induced by a revisited paradigm of voluntary ethanol consumption in adult male and female Sprague-Dawley rats
title_sort Evaluating signs of hippocampal neurotoxicity induced by a revisited paradigm of voluntary ethanol consumption in adult male and female Sprague-Dawley rats
dc.creator.none.fl_str_mv Colom-Rocha, Carles
Bis-Humbert, Cristian
García-Fuster, M Julia
author Colom-Rocha, Carles
author_facet Colom-Rocha, Carles
Bis-Humbert, Cristian
García-Fuster, M Julia
author_role author
author2 Bis-Humbert, Cristian
García-Fuster, M Julia
author2_role author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Male
Alcohol Drinking
Rats
Female
Alcoholism
Animals
Hippocampus
Rats, Sprague-Dawley
Ethanol
Animales
Ratas
Ratas Sprague-Dawley
Etanol
Hipocampo
Femenino
Alcoholismo
Consumo de Bebidas Alcohólicas
Masculino
topic Male
Alcohol Drinking
Rats
Female
Alcoholism
Animals
Hippocampus
Rats, Sprague-Dawley
Ethanol
Animales
Ratas
Ratas Sprague-Dawley
Etanol
Hipocampo
Femenino
Alcoholismo
Consumo de Bebidas Alcohólicas
Masculino
description Binge alcohol drinking is considered a prominent risk factor for the development of alcohol-use disorders, and could be model in rodents through the standard two-bottle preference choice test. The goal was to recreate an intermittent use of alcohol during 3 consecutive days each week to ascertain its potential impact on hippocampal neurotoxicity (neurogenesis and other neuroplasticity markers), and including sex as a biological variable, given the well-known sex differences in alcohol consumption. Ethanol access was granted to adult Sprague-Dawley rats for 3 consecutive days per week, followed by 4 days of withdrawal, during 6 weeks, mimicking the most common pattern of intake in people, drinking over the weekends in an intensive manner. Hippocampal samples were collected to evaluate signs of neurotoxicity. Female rats consumed significantly more ethanol than males, although intake did not escalate over time. Ethanol preference levels remained below 40% over time and did not differ between sexes. Moderate signs of ethanol neurotoxicity were observed in hippocampus at the level of decreased neuronal progenitors (NeuroD + cells), and these effects were independent of sex. No other signs of neurotoxicity were induced by ethanol voluntary consumption when measured through several key cell fate markers (i.e., FADD, Cyt c, Cdk5, NF-L) by western blot analysis. Overall, the present results suggest that even though we modeled a situation where no escalation in ethanol intake occurred across time, mild signs of neurotoxicity emerged, suggesting that even the use of ethanol during adulthood in a recreational way could lead to certain brain harm.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-04-01
2023
2023-04-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.13003/18903
url https://hdl.handle.net/20.500.13003/18903
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Docusalut
instname:Conselleria de Salut i Consum del Govern de les Illes Balears
instname_str Conselleria de Salut i Consum del Govern de les Illes Balears
reponame_str Docusalut
collection Docusalut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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