Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective Against Urinary Tract Bacteria
Anthocyanins are extensively studied for their health-related properties, including antibacterial activity against urinary tract infections (UTI). Among common fruits, blueberries, with their remarkable antioxidant capacity, are one of the richest sources. Anthocyanin-rich extracts were obtained fro...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2020 |
| País: | España |
| Recursos: | Universidad de Sevilla (US) |
| Repositório: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/97673 |
| Acesso em linha: | https://hdl.handle.net/11441/97673 https://doi.org/10.3390/antiox9060478 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Escherichia coli Klebsiella pneumoniae Micrococcus ORAC Providencia stuartii Pseudomonas aeruginosa UHPLC-MS/MS Orbitrap antibacterial activity cyanidin-3-rutinoside |
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Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective Against Urinary Tract BacteriaCerezo López, Ana BelénCătunescu, Giorgiana M.Martínez-Pais González, MercedesHornedo Ortega, RuthPop, Carmen RRusu, Crina ClaudiaChirilă, FloreRotar, Ancuța MGarcía Parrilla, María del CarmenTroncoso González, Ana MaríaEscherichia coliKlebsiella pneumoniaeMicrococcusORACProvidencia stuartiiPseudomonas aeruginosaUHPLC-MS/MS Orbitrapantibacterial activitycyanidin-3-rutinosideAnthocyanins are extensively studied for their health-related properties, including antibacterial activity against urinary tract infections (UTI). Among common fruits, blueberries, with their remarkable antioxidant capacity, are one of the richest sources. Anthocyanin-rich extracts were obtained from four varieties: Snowchaser, Star, Stella Blue and Cristina Blue, grown in the hot climate of Southern Spain. Their total anthocyanins contents (TAC) were determined spectrophotometrically, and the anthocyanin profile by ultra high performance liquid chromatography - tandem mass spectrometer (UHPLC-MS/MS). Their antioxidant activity was assessed by oxygen radical absorbance capacity (ORAC) assay, while antibacterial activity against strains isolated from UTI patients was assessed in vitro, helping to select the varieties with the highest bioactive potential. Star showed the highest TAC and antioxidant activity (1663 ± 159 mg of cyanidin-3-O-glucoside (cy-3-O-glu) equivalents/100 g fresh weight (FW), 6345 ± 601 μmol Trolox equivalents (TE)/100 g FW, respectively), followed by Cristina Blue, Stella Blue and Snowchaser. As far as we know, this is the first time that cyanidin-3-rutinoside has been identified in blueberries. The extracts inhibited all the tested strains, MICs ranging from 0.4 mg/mL (for Stella Blue extract against UTI P. aeruginosa) to 9.5 mg/mL (for all extracts against UTI K. pneumoniae ssp. pneumoniae). This is the first study that assessed in vitro the antibacterial activity of blueberries against Klebsiella pneumoniae, Providencia stuartii and Micrococcus spp. strains isolated from UTI.España, University of Sevilla, VI Plan Propio de Investigación y Transferencia (VIPPIT‐2019‐I.5)MDPINutrición y Bromatología, Toxicología y Medicina LegalUniversidad de Sevilla2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/97673https://doi.org/10.3390/antiox9060478reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésAntioxidants, 9 (6), 1-22.VIPPIT‐2019‐I.5https://doi.org/10.3390/antiox9060478info:eu-repo/semantics/openAccessoai:idus.us.es:11441/976732026-06-17T12:51:07Z |
| dc.title.none.fl_str_mv |
Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective Against Urinary Tract Bacteria |
| title |
Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective Against Urinary Tract Bacteria |
| spellingShingle |
Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective Against Urinary Tract Bacteria Cerezo López, Ana Belén Escherichia coli Klebsiella pneumoniae Micrococcus ORAC Providencia stuartii Pseudomonas aeruginosa UHPLC-MS/MS Orbitrap antibacterial activity cyanidin-3-rutinoside |
| title_short |
Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective Against Urinary Tract Bacteria |
| title_full |
Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective Against Urinary Tract Bacteria |
| title_fullStr |
Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective Against Urinary Tract Bacteria |
| title_full_unstemmed |
Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective Against Urinary Tract Bacteria |
| title_sort |
Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective Against Urinary Tract Bacteria |
| dc.creator.none.fl_str_mv |
Cerezo López, Ana Belén Cătunescu, Giorgiana M. Martínez-Pais González, Mercedes Hornedo Ortega, Ruth Pop, Carmen R Rusu, Crina Claudia Chirilă, Flore Rotar, Ancuța M García Parrilla, María del Carmen Troncoso González, Ana María |
| author |
Cerezo López, Ana Belén |
| author_facet |
Cerezo López, Ana Belén Cătunescu, Giorgiana M. Martínez-Pais González, Mercedes Hornedo Ortega, Ruth Pop, Carmen R Rusu, Crina Claudia Chirilă, Flore Rotar, Ancuța M García Parrilla, María del Carmen Troncoso González, Ana María |
| author_role |
author |
| author2 |
Cătunescu, Giorgiana M. Martínez-Pais González, Mercedes Hornedo Ortega, Ruth Pop, Carmen R Rusu, Crina Claudia Chirilă, Flore Rotar, Ancuța M García Parrilla, María del Carmen Troncoso González, Ana María |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Nutrición y Bromatología, Toxicología y Medicina Legal Universidad de Sevilla |
| dc.subject.none.fl_str_mv |
Escherichia coli Klebsiella pneumoniae Micrococcus ORAC Providencia stuartii Pseudomonas aeruginosa UHPLC-MS/MS Orbitrap antibacterial activity cyanidin-3-rutinoside |
| topic |
Escherichia coli Klebsiella pneumoniae Micrococcus ORAC Providencia stuartii Pseudomonas aeruginosa UHPLC-MS/MS Orbitrap antibacterial activity cyanidin-3-rutinoside |
| description |
Anthocyanins are extensively studied for their health-related properties, including antibacterial activity against urinary tract infections (UTI). Among common fruits, blueberries, with their remarkable antioxidant capacity, are one of the richest sources. Anthocyanin-rich extracts were obtained from four varieties: Snowchaser, Star, Stella Blue and Cristina Blue, grown in the hot climate of Southern Spain. Their total anthocyanins contents (TAC) were determined spectrophotometrically, and the anthocyanin profile by ultra high performance liquid chromatography - tandem mass spectrometer (UHPLC-MS/MS). Their antioxidant activity was assessed by oxygen radical absorbance capacity (ORAC) assay, while antibacterial activity against strains isolated from UTI patients was assessed in vitro, helping to select the varieties with the highest bioactive potential. Star showed the highest TAC and antioxidant activity (1663 ± 159 mg of cyanidin-3-O-glucoside (cy-3-O-glu) equivalents/100 g fresh weight (FW), 6345 ± 601 μmol Trolox equivalents (TE)/100 g FW, respectively), followed by Cristina Blue, Stella Blue and Snowchaser. As far as we know, this is the first time that cyanidin-3-rutinoside has been identified in blueberries. The extracts inhibited all the tested strains, MICs ranging from 0.4 mg/mL (for Stella Blue extract against UTI P. aeruginosa) to 9.5 mg/mL (for all extracts against UTI K. pneumoniae ssp. pneumoniae). This is the first study that assessed in vitro the antibacterial activity of blueberries against Klebsiella pneumoniae, Providencia stuartii and Micrococcus spp. strains isolated from UTI. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/11441/97673 https://doi.org/10.3390/antiox9060478 |
| url |
https://hdl.handle.net/11441/97673 https://doi.org/10.3390/antiox9060478 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Antioxidants, 9 (6), 1-22. VIPPIT‐2019‐I.5 https://doi.org/10.3390/antiox9060478 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf |
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MDPI |
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MDPI |
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reponame:idUS. Depósito de Investigación de la Universidad de Sevilla instname:Universidad de Sevilla (US) |
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Universidad de Sevilla (US) |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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