Identification of regulatory molecules involved in pollen development and ovary growth in Solanum lycopersicum
[EN] In angiosperms, fruits serve the purpose of protecting developing seeds and facilitating their dispersal. After flower development, pollination and fertilization trigger the growth of the ovary into a fruit. However, fruit set and enlargement can occur independently of these two processes, resu...
| Autor: | |
|---|---|
| Formato: | tesis doctoral |
| Fecha de publicación: | 2026 |
| País: | España |
| Recursos: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:dnet:riunet______::4234f610cae56602266239de79804b12 |
| Acesso em linha: | https://riunet.upv.es/handle/10251/233735 |
| Access Level: | acceso abierto |
| Palavra-chave: | Desarrollo Fisiología vegetal Tomate Solanum lycopersicum Plant development Anther Pollen Microgametogenesis Heat stress Tomato Parthenocarpy 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
| id |
ES_ccd7dd4805eb2d63dddb92860f27a3fc |
|---|---|
| oai_identifier_str |
oai:dnet:riunet______::4234f610cae56602266239de79804b12 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| dc.title.none.fl_str_mv |
Identification of regulatory molecules involved in pollen development and ovary growth in Solanum lycopersicum |
| title |
Identification of regulatory molecules involved in pollen development and ovary growth in Solanum lycopersicum |
| spellingShingle |
Identification of regulatory molecules involved in pollen development and ovary growth in Solanum lycopersicum Salazar-Sarasúa, Blanca Desarrollo Fisiología vegetal Tomate Solanum lycopersicum Plant development Anther Pollen Microgametogenesis Heat stress Tomato Parthenocarpy 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
| title_short |
Identification of regulatory molecules involved in pollen development and ovary growth in Solanum lycopersicum |
| title_full |
Identification of regulatory molecules involved in pollen development and ovary growth in Solanum lycopersicum |
| title_fullStr |
Identification of regulatory molecules involved in pollen development and ovary growth in Solanum lycopersicum |
| title_full_unstemmed |
Identification of regulatory molecules involved in pollen development and ovary growth in Solanum lycopersicum |
| title_sort |
Identification of regulatory molecules involved in pollen development and ovary growth in Solanum lycopersicum |
| dc.creator.none.fl_str_mv |
Salazar-Sarasúa, Blanca |
| author |
Salazar-Sarasúa, Blanca |
| author_facet |
Salazar-Sarasúa, Blanca |
| author_role |
author |
| dc.contributor.none.fl_str_mv |
Gómez Mena, María Concepción Instituto Universitario Mixto de Biología Molecular y Celular de Plantas Agencia Estatal de Investigación Consejo Superior de Investigaciones Científicas Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Desarrollo Fisiología vegetal Tomate Solanum lycopersicum Plant development Anther Pollen Microgametogenesis Heat stress Tomato Parthenocarpy 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
| topic |
Desarrollo Fisiología vegetal Tomate Solanum lycopersicum Plant development Anther Pollen Microgametogenesis Heat stress Tomato Parthenocarpy 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
| description |
[EN] In angiosperms, fruits serve the purpose of protecting developing seeds and facilitating their dispersal. After flower development, pollination and fertilization trigger the growth of the ovary into a fruit. However, fruit set and enlargement can occur independently of these two processes, resulting in seedless (parthenocarpic) fruits. In the domesticated tomato (Solanum lycopersicum), male sterility can induce parthenocarpic fruit development, providing a link between the formation of the male gametophyte and ovary growth. Pollen and anther development are highly coordinated processes that are largely conserved across angiosperms. However, in tomato, the specific network of genes involved in anther development is not fully characterized. The generation and study of parthenocarpic mutants has proven a powerful tool for understanding the mechanisms that connect male gametogenesis and ovary growth in this species, as well as for investigating the origin of parthenocarpy. Furthermore, the male gametophyte is especially sensitive to adverse environmental conditions, and increasing knowledge on pollen development could provide insight into strategies to maintain fertility under rising temperatures. In this work, we have identified SlTPD1 as an essential gene for tapetum formation in the anther and SlTDF1 as a regulator of tapetal function. Transcriptomic and biochemical analysis conducted in Sltpd1 mutants lacking tapetum highlighted the importance of tapetal tissue in maintaining redox homeostasis during male gametogenesis. Both Sltpd1 and Sltdf1 mutants were male-sterile and, subsequently, developed parthenocarpic fruits. However, male-sterile SpTDF1 mutants obtained in the wild relative Solanum pimpinellifolium background did not exhibit parthenocarpy. Bioinformatic analyses identified deletions in the promoters of genes involved in gibberellin biosynthesis, which affect the binding of repressor proteins. These results point to recent changes in regulatory sequences, indicating that parthenocarpy in tomato is a trait that likely originated during domestication. Additionally, a biotechnology approach was developed to enhance pollen thermotolerance. Transcriptomic analyses identified the ascorbate peroxidase SlAPX5 gene as part of a regulatory network controlling redox homeostasis in the anther. A heat-inducible expression of SlAPX5 was introduced in Arabidopsis and tomato by genetic transformation. In vivo and in vitro experiments confirm that ROS detoxification mediated by peroxidase activity conferred heat tolerance to vegetative and reproductive tissues, preserving fertility and securing plant yield. |
| publishDate |
2026 |
| dc.date.none.fl_str_mv |
2026 2026-03-26 2026 2026-02-20 |
| dc.type.none.fl_str_mv |
doctoral thesis http://purl.org/coar/resource_type/c_db06 AM http://purl.org/coar/version/c_ab4af688f83e57aa |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
| format |
doctoralThesis |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/233735 |
| url |
https://riunet.upv.es/handle/10251/233735 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Proyectos I+D+i Retos de investigación RTI2018-094280-B-I00 EDICION DE GENES DE TOMATE IMPLICADOS EN LA GAMETOGENESIS MASCULINA: DISECCION DEL POTENCIAL BIOTECNOLOGICO DE LA ANDROESTERILIDAD EN LA OBTENCION DE FRUTOS SIN SEMILLAS Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Proyectos de I+D+I (Generación de Conocimiento y Retos Investigación) PID2021-123705OB-I00 Comisión Sectorial de Investigación Científica, Uruguay https://doi.org/10.13039/501100003339 iMOVE IMOVE23239 Evaluación de la adquisición de termotolerancia en polen mediante la activación de peroxidasas en respuesta a calor |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Universitat Politècnica de València |
| publisher.none.fl_str_mv |
Universitat Politècnica de València |
| dc.source.none.fl_str_mv |
reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
| instname_str |
Universitat Politècnica de València (UPV) |
| reponame_str |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| collection |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869419756472238080 |
| spelling |
Identification of regulatory molecules involved in pollen development and ovary growth in Solanum lycopersicumSalazar-Sarasúa, BlancaDesarrolloFisiología vegetalTomateSolanum lycopersicumPlant developmentAntherPollenMicrogametogenesisHeat stressTomatoParthenocarpy02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible[EN] In angiosperms, fruits serve the purpose of protecting developing seeds and facilitating their dispersal. After flower development, pollination and fertilization trigger the growth of the ovary into a fruit. However, fruit set and enlargement can occur independently of these two processes, resulting in seedless (parthenocarpic) fruits. In the domesticated tomato (Solanum lycopersicum), male sterility can induce parthenocarpic fruit development, providing a link between the formation of the male gametophyte and ovary growth. Pollen and anther development are highly coordinated processes that are largely conserved across angiosperms. However, in tomato, the specific network of genes involved in anther development is not fully characterized. The generation and study of parthenocarpic mutants has proven a powerful tool for understanding the mechanisms that connect male gametogenesis and ovary growth in this species, as well as for investigating the origin of parthenocarpy. Furthermore, the male gametophyte is especially sensitive to adverse environmental conditions, and increasing knowledge on pollen development could provide insight into strategies to maintain fertility under rising temperatures. In this work, we have identified SlTPD1 as an essential gene for tapetum formation in the anther and SlTDF1 as a regulator of tapetal function. Transcriptomic and biochemical analysis conducted in Sltpd1 mutants lacking tapetum highlighted the importance of tapetal tissue in maintaining redox homeostasis during male gametogenesis. Both Sltpd1 and Sltdf1 mutants were male-sterile and, subsequently, developed parthenocarpic fruits. However, male-sterile SpTDF1 mutants obtained in the wild relative Solanum pimpinellifolium background did not exhibit parthenocarpy. Bioinformatic analyses identified deletions in the promoters of genes involved in gibberellin biosynthesis, which affect the binding of repressor proteins. These results point to recent changes in regulatory sequences, indicating that parthenocarpy in tomato is a trait that likely originated during domestication. Additionally, a biotechnology approach was developed to enhance pollen thermotolerance. Transcriptomic analyses identified the ascorbate peroxidase SlAPX5 gene as part of a regulatory network controlling redox homeostasis in the anther. A heat-inducible expression of SlAPX5 was introduced in Arabidopsis and tomato by genetic transformation. In vivo and in vitro experiments confirm that ROS detoxification mediated by peroxidase activity conferred heat tolerance to vegetative and reproductive tissues, preserving fertility and securing plant yield.El trabajo realizado durante esta tesis doctoral ha sido financiado por el Ministerio de Ciencia, Innovación y Universidades (RTI2018-094280-B-I00 concedida a C.G.-M) y por el Fondo Europeo de Desarrollo Regional (FEDER) “A way of making Europe” (PID2021-123705OB-I00 concedida a C.G.-M.). Además, la estancia de investigación ha sido posible gracias a la financiación del programa iMOVE del Consejo Superior de Investigaciones Científicas (CSIC) (IMOVE23239 concedida a B.S.-S).Universitat Politècnica de ValènciaGómez Mena, María ConcepciónInstituto Universitario Mixto de Biología Molecular y Celular de PlantasAgencia Estatal de InvestigaciónConsejo Superior de Investigaciones CientíficasRepositorio Institucional de la Universitat Politècnica de València Riunet20262026-03-2620262026-02-20doctoral thesishttp://purl.org/coar/resource_type/c_db06AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/233735reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Proyectos I+D+i Retos de investigación RTI2018-094280-B-I00 EDICION DE GENES DE TOMATE IMPLICADOS EN LA GAMETOGENESIS MASCULINA: DISECCION DEL POTENCIAL BIOTECNOLOGICO DE LA ANDROESTERILIDAD EN LA OBTENCION DE FRUTOS SIN SEMILLASAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Proyectos de I+D+I (Generación de Conocimiento y Retos Investigación) PID2021-123705OB-I00Comisión Sectorial de Investigación Científica, Uruguay https://doi.org/10.13039/501100003339 iMOVE IMOVE23239 Evaluación de la adquisición de termotolerancia en polen mediante la activación de peroxidasas en respuesta a caloropen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:dnet:riunet______::4234f610cae56602266239de79804b122026-06-13T07:49:27Z |
| score |
15,811543 |