Interactions of human galectins with Trypanosoma cruzi

We report here the specific interaction between several members of the human galectin family with the three developmental stages of several genetic lineages of the protozoan parasite Trypanosoma cruzi.We provide data of specific and differential binding of human galectin (gal)-1, -3, -4, -7 and -8 t...

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Detalles Bibliográficos
Autores: Pineda, Miguel Ángel, Corvo, Laura, Soto, Manuel, Fresno, Manuel, Bonay, Pedro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/139570
Acceso en línea:http://hdl.handle.net/10261/139570
Access Level:acceso abierto
Palabra clave:Trypanosoma cruzi
host cell adhesion
discrete typing units
galectin
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spelling Interactions of human galectins with Trypanosoma cruziPineda, Miguel ÁngelCorvo, LauraSoto, ManuelFresno, ManuelBonay, PedroTrypanosoma cruzihost cell adhesiondiscrete typing unitsgalectinWe report here the specific interaction between several members of the human galectin family with the three developmental stages of several genetic lineages of the protozoan parasite Trypanosoma cruzi.We provide data of specific and differential binding of human galectin (gal)-1, -3, -4, -7 and -8 to 14 strains of T. cruzi that belong to the six genetic lineages representing the genetic diversity of the parasite. It is shown that galectins preferentially bind forms present in the host, trypomastigotes and amastigotes, compared with the non-infective epimastigote present on the intestinal tract of the vector, reflecting the changes on glycosylation that occur during the metacyclogenesis and amastigogenesis process. Also, it is evidenced that galectin binding to the parasites promotes binding to the host cells and higher infection rates. In addition, evidence is provided indicating that the intracellular amastigotes may take over the cytosolic pool of some galectins when released to the extracellular medium. Finally, by applying unweighted pair group method analysis to the galectin-binding profile to either cell-derived trypomastigotes or amastigotes,we showthat the differential-binding profile by the host galectins to the six lineages resembles the clustering based in genetic data. Therefore, the differential-binding profile for the six lineages could have implications in the immunopathology of Chagas’ disease, affecting the complex network of immune responses on which galectins mediate, thus providing linkage clues to the notion that different lineages may be related to different clinical forms of the disease.Ministerio de Sanidad and Fundación Ramón ArecesPeer ReviewedOxford University PressMinisterio de Sanidad, Servicios Sociales e Igualdad (España)Fundación Ramón ArecesConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2016201620152016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/139570reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1395702026-05-22T06:33:51Z
dc.title.none.fl_str_mv Interactions of human galectins with Trypanosoma cruzi
title Interactions of human galectins with Trypanosoma cruzi
spellingShingle Interactions of human galectins with Trypanosoma cruzi
Pineda, Miguel Ángel
Trypanosoma cruzi
host cell adhesion
discrete typing units
galectin
title_short Interactions of human galectins with Trypanosoma cruzi
title_full Interactions of human galectins with Trypanosoma cruzi
title_fullStr Interactions of human galectins with Trypanosoma cruzi
title_full_unstemmed Interactions of human galectins with Trypanosoma cruzi
title_sort Interactions of human galectins with Trypanosoma cruzi
dc.creator.none.fl_str_mv Pineda, Miguel Ángel
Corvo, Laura
Soto, Manuel
Fresno, Manuel
Bonay, Pedro
author Pineda, Miguel Ángel
author_facet Pineda, Miguel Ángel
Corvo, Laura
Soto, Manuel
Fresno, Manuel
Bonay, Pedro
author_role author
author2 Corvo, Laura
Soto, Manuel
Fresno, Manuel
Bonay, Pedro
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Sanidad, Servicios Sociales e Igualdad (España)
Fundación Ramón Areces
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Trypanosoma cruzi
host cell adhesion
discrete typing units
galectin
topic Trypanosoma cruzi
host cell adhesion
discrete typing units
galectin
description We report here the specific interaction between several members of the human galectin family with the three developmental stages of several genetic lineages of the protozoan parasite Trypanosoma cruzi.We provide data of specific and differential binding of human galectin (gal)-1, -3, -4, -7 and -8 to 14 strains of T. cruzi that belong to the six genetic lineages representing the genetic diversity of the parasite. It is shown that galectins preferentially bind forms present in the host, trypomastigotes and amastigotes, compared with the non-infective epimastigote present on the intestinal tract of the vector, reflecting the changes on glycosylation that occur during the metacyclogenesis and amastigogenesis process. Also, it is evidenced that galectin binding to the parasites promotes binding to the host cells and higher infection rates. In addition, evidence is provided indicating that the intracellular amastigotes may take over the cytosolic pool of some galectins when released to the extracellular medium. Finally, by applying unweighted pair group method analysis to the galectin-binding profile to either cell-derived trypomastigotes or amastigotes,we showthat the differential-binding profile by the host galectins to the six lineages resembles the clustering based in genetic data. Therefore, the differential-binding profile for the six lineages could have implications in the immunopathology of Chagas’ disease, affecting the complex network of immune responses on which galectins mediate, thus providing linkage clues to the notion that different lineages may be related to different clinical forms of the disease.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/139570
url http://hdl.handle.net/10261/139570
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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