Identifying miRNA-mRNA regulatory networks on extreme n-6/n-3 polyunsaturated fatty acid ratio expression profiles in porcine skeletal muscle

Omega-3 (n-3) and omega-6 (n-6) polyunsaturated fatty acids (PUFAs) are essential fatty acids with antagonistic inflammatory functions that play vital roles in metabolic health and immune response. Current commercial swine diets tend to over-supplement with n-6 PUFAs, which may increase the likeliho...

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Autores: Manaig, Yron Joseph Yabut, Criado-Mesas, Lourdes, Esteve-Codina, Anna, Mármol-Sánchez, Emilio, Castelló, Anna, Sánchez, Armand, Folch, Josep M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/57320
Acceso en línea:http://hdl.handle.net/10230/57320
http://dx.doi.org/10.1371/journal.pone.0283231
Access Level:acceso abierto
Palabra clave:MicroRNAs
Swine
Gene expression
Adrenergic signal transduction
Skeletal muscles
Gene regulation
Messenger RNA
RNA sequencing
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spelling Identifying miRNA-mRNA regulatory networks on extreme n-6/n-3 polyunsaturated fatty acid ratio expression profiles in porcine skeletal muscleManaig, Yron Joseph YabutCriado-Mesas, LourdesEsteve-Codina, AnnaMármol-Sánchez, EmilioCastelló, AnnaSánchez, ArmandFolch, Josep M.MicroRNAsSwineGene expressionAdrenergic signal transductionSkeletal musclesGene regulationMessenger RNARNA sequencingOmega-3 (n-3) and omega-6 (n-6) polyunsaturated fatty acids (PUFAs) are essential fatty acids with antagonistic inflammatory functions that play vital roles in metabolic health and immune response. Current commercial swine diets tend to over-supplement with n-6 PUFAs, which may increase the likelihood of developing inflammatory diseases and affect the overall well-being of the animals. However, it is still poorly understood how n-6/n-3 PUFA ratios affect the porcine transcriptome expression and how messenger RNAs (mRNAs) and microRNAs (miRNAs) might regulate biological processes related to PUFA metabolism. On account of this, we selected a total of 20 Iberian × Duroc crossbred pigs with extreme values for n-6/n-3 FA ratio (10 high vs 10 low), and longissimus dorsi muscle samples were used to identify differentially expressed mRNAs and miRNAs. The observed differentially expressed mRNAs were associated to biological pathways related to muscle growth and immunomodulation, while the differentially expressed microRNAs (ssc-miR-30a-3p, ssc-miR-30e-3p, ssc-miR-15b and ssc-miR-7142-3p) were correlated to adipogenesis and immunity. Relevant miRNA-to-mRNA regulatory networks were also predicted (i.e., mir15b to ARRDC3; mir-7142-3p to METTL21C), and linked to lipolysis, obesity, myogenesis, and protein degradation. The n-6/n-3 PUFA ratio differences in pig skeletal muscle revealed genes, miRNAs and enriched pathways involved in lipid metabolism, cell proliferation and inflammation.This work was supported by the Spanish Ministerio de Ciencia e Innovación (MICINN) and the Fondo Europeo de Desarrollo Regional (FEDER) with project references: AGL2017-82641-R and PID2020-112677RB-C22. YJYM is financially supported by the European Union Horizon 2020 Research and Innovation programme H2020-MSCA-ITN-2017- EJD: Marie Skłodowska-Curie Innovative Training Networks (European Joint Doctorate) – Grant agreement nº: 765423 – MANNA. LCM was funded with a FPI grant from the AGL2014-56369-C2 project. EMS was funded with a PhD fellowship FPU15/01733 awarded by the Spanish Ministerio de Educación, Cultura y Deporte (MECD). We also acknowledge the support of the Spanish Ministerio de Economía y Competitividad for the “Severo Ochoa” Programme for Centres of Excellence in R&D (Project No. CEX2019-000902-S) to the Centre for Research in Agricultural Genomics (CRAG) and to the programmes of Centres de Recerca de Catalunya (CERCA). The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.Public Library of Science (PLoS)202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/57320http://dx.doi.org/10.1371/journal.pone.0283231reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPLoS One. 2023 May 4;18(5):e0283231info:eu-repo/grantAgreement/EC/H2020/765423info:eu-repo/grantAgreement/ES/2PE/AGL2017-82641-Rinfo:eu-repo/grantAgreement/ES/2PE/PID2020-112677RB-C22info:eu-repo/grantAgreement/ES/1PE/AGL2014-56369-C2© 2023 Manaig et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/573202026-05-29T05:05:01Z
dc.title.none.fl_str_mv Identifying miRNA-mRNA regulatory networks on extreme n-6/n-3 polyunsaturated fatty acid ratio expression profiles in porcine skeletal muscle
title Identifying miRNA-mRNA regulatory networks on extreme n-6/n-3 polyunsaturated fatty acid ratio expression profiles in porcine skeletal muscle
spellingShingle Identifying miRNA-mRNA regulatory networks on extreme n-6/n-3 polyunsaturated fatty acid ratio expression profiles in porcine skeletal muscle
Manaig, Yron Joseph Yabut
MicroRNAs
Swine
Gene expression
Adrenergic signal transduction
Skeletal muscles
Gene regulation
Messenger RNA
RNA sequencing
title_short Identifying miRNA-mRNA regulatory networks on extreme n-6/n-3 polyunsaturated fatty acid ratio expression profiles in porcine skeletal muscle
title_full Identifying miRNA-mRNA regulatory networks on extreme n-6/n-3 polyunsaturated fatty acid ratio expression profiles in porcine skeletal muscle
title_fullStr Identifying miRNA-mRNA regulatory networks on extreme n-6/n-3 polyunsaturated fatty acid ratio expression profiles in porcine skeletal muscle
title_full_unstemmed Identifying miRNA-mRNA regulatory networks on extreme n-6/n-3 polyunsaturated fatty acid ratio expression profiles in porcine skeletal muscle
title_sort Identifying miRNA-mRNA regulatory networks on extreme n-6/n-3 polyunsaturated fatty acid ratio expression profiles in porcine skeletal muscle
dc.creator.none.fl_str_mv Manaig, Yron Joseph Yabut
Criado-Mesas, Lourdes
Esteve-Codina, Anna
Mármol-Sánchez, Emilio
Castelló, Anna
Sánchez, Armand
Folch, Josep M.
author Manaig, Yron Joseph Yabut
author_facet Manaig, Yron Joseph Yabut
Criado-Mesas, Lourdes
Esteve-Codina, Anna
Mármol-Sánchez, Emilio
Castelló, Anna
Sánchez, Armand
Folch, Josep M.
author_role author
author2 Criado-Mesas, Lourdes
Esteve-Codina, Anna
Mármol-Sánchez, Emilio
Castelló, Anna
Sánchez, Armand
Folch, Josep M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv MicroRNAs
Swine
Gene expression
Adrenergic signal transduction
Skeletal muscles
Gene regulation
Messenger RNA
RNA sequencing
topic MicroRNAs
Swine
Gene expression
Adrenergic signal transduction
Skeletal muscles
Gene regulation
Messenger RNA
RNA sequencing
description Omega-3 (n-3) and omega-6 (n-6) polyunsaturated fatty acids (PUFAs) are essential fatty acids with antagonistic inflammatory functions that play vital roles in metabolic health and immune response. Current commercial swine diets tend to over-supplement with n-6 PUFAs, which may increase the likelihood of developing inflammatory diseases and affect the overall well-being of the animals. However, it is still poorly understood how n-6/n-3 PUFA ratios affect the porcine transcriptome expression and how messenger RNAs (mRNAs) and microRNAs (miRNAs) might regulate biological processes related to PUFA metabolism. On account of this, we selected a total of 20 Iberian × Duroc crossbred pigs with extreme values for n-6/n-3 FA ratio (10 high vs 10 low), and longissimus dorsi muscle samples were used to identify differentially expressed mRNAs and miRNAs. The observed differentially expressed mRNAs were associated to biological pathways related to muscle growth and immunomodulation, while the differentially expressed microRNAs (ssc-miR-30a-3p, ssc-miR-30e-3p, ssc-miR-15b and ssc-miR-7142-3p) were correlated to adipogenesis and immunity. Relevant miRNA-to-mRNA regulatory networks were also predicted (i.e., mir15b to ARRDC3; mir-7142-3p to METTL21C), and linked to lipolysis, obesity, myogenesis, and protein degradation. The n-6/n-3 PUFA ratio differences in pig skeletal muscle revealed genes, miRNAs and enriched pathways involved in lipid metabolism, cell proliferation and inflammation.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/57320
http://dx.doi.org/10.1371/journal.pone.0283231
url http://hdl.handle.net/10230/57320
http://dx.doi.org/10.1371/journal.pone.0283231
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS One. 2023 May 4;18(5):e0283231
info:eu-repo/grantAgreement/EC/H2020/765423
info:eu-repo/grantAgreement/ES/2PE/AGL2017-82641-R
info:eu-repo/grantAgreement/ES/2PE/PID2020-112677RB-C22
info:eu-repo/grantAgreement/ES/1PE/AGL2014-56369-C2
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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