Modelling the steady-state current at the inlaid disc microelectrode for homogeneous mediated enzyme catalysed reactions

The steady-state currents at an inlaid microdisc electrode have been modelled for a redox mediated enzyme catalysed reaction (such as the glucose/glucose oxidase/ferrocene system) in which all the components are present in homogeneous solution and the reaction of the redox mediator at the electrode...

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Autores: Galceran i Nogués, Josep, Taylor, S. L., Bartlett, P. N.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2001
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/48899
Acceso en línea:https://doi.org/10.1016/S0022-0728(01)00503-4
http://hdl.handle.net/10459.1/48899
Access Level:acceso abierto
Palabra clave:Electroquímica
Anàlisi electroquímica
Electrochemistry
Electrochemical analysis
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spelling Modelling the steady-state current at the inlaid disc microelectrode for homogeneous mediated enzyme catalysed reactionsGalceran i Nogués, JosepTaylor, S. L.Bartlett, P. N.ElectroquímicaAnàlisi electroquímicaElectrochemistryElectrochemical analysisThe steady-state currents at an inlaid microdisc electrode have been modelled for a redox mediated enzyme catalysed reaction (such as the glucose/glucose oxidase/ferrocene system) in which all the components are present in homogeneous solution and the reaction of the redox mediator at the electrode is assumed to be either reversible or mass transport limited. The numerical solution for the non-linear system in the axisymmetrical geometry of the inlaid disc is achieved by using the finite element method in an iterative scheme. The resulting concentration and reaction profiles provide useful insight into the process, and show for some parameter values the formation of an almost spherical, sharp reaction layer or shell, whose position can be predicted approximately. Conditions under which the scheme reverts to the pseudo-first-order EC′ mechanism and expressions to interpret the corresponding current are discussed. A simple approximate expression is worked out under the assumption of constant substrate concentration on the electrode surface. This approximate expression is useful in determining a combination of the parameters from the slope of measurements at low substrate concentration. The effect of some parameters is discussed in detail, leading to two suggestions which can improve the analytical performance: (i) use of the lowest mediator concentration compatible with the background current and (ii) use of the largest microelectrode which reaches steady state in a reasonable time. Experimental measurements using ferrocene monocarboxylic acid as the mediator for the glucose/glucose oxidase system agree with the model and confirm the order of magnitude of the previously reported parameter values.Elsevier2015201520012015info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1016/S0022-0728(01)00503-4http://hdl.handle.net/10459.1/48899http://hdl.handle.net/10459.1/48899reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/S0022-0728(01)00503-4Journal of Electroanalytical Chemistry, 2001, vol. 506, num. 2, p. 65-81(c) Elsevier, 2001info:eu-repo/semantics/openAccessoai:recercat.cat:10459.1/488992026-05-29T05:05:01Z
dc.title.none.fl_str_mv Modelling the steady-state current at the inlaid disc microelectrode for homogeneous mediated enzyme catalysed reactions
title Modelling the steady-state current at the inlaid disc microelectrode for homogeneous mediated enzyme catalysed reactions
spellingShingle Modelling the steady-state current at the inlaid disc microelectrode for homogeneous mediated enzyme catalysed reactions
Galceran i Nogués, Josep
Electroquímica
Anàlisi electroquímica
Electrochemistry
Electrochemical analysis
title_short Modelling the steady-state current at the inlaid disc microelectrode for homogeneous mediated enzyme catalysed reactions
title_full Modelling the steady-state current at the inlaid disc microelectrode for homogeneous mediated enzyme catalysed reactions
title_fullStr Modelling the steady-state current at the inlaid disc microelectrode for homogeneous mediated enzyme catalysed reactions
title_full_unstemmed Modelling the steady-state current at the inlaid disc microelectrode for homogeneous mediated enzyme catalysed reactions
title_sort Modelling the steady-state current at the inlaid disc microelectrode for homogeneous mediated enzyme catalysed reactions
dc.creator.none.fl_str_mv Galceran i Nogués, Josep
Taylor, S. L.
Bartlett, P. N.
author Galceran i Nogués, Josep
author_facet Galceran i Nogués, Josep
Taylor, S. L.
Bartlett, P. N.
author_role author
author2 Taylor, S. L.
Bartlett, P. N.
author2_role author
author
dc.subject.none.fl_str_mv Electroquímica
Anàlisi electroquímica
Electrochemistry
Electrochemical analysis
topic Electroquímica
Anàlisi electroquímica
Electrochemistry
Electrochemical analysis
description The steady-state currents at an inlaid microdisc electrode have been modelled for a redox mediated enzyme catalysed reaction (such as the glucose/glucose oxidase/ferrocene system) in which all the components are present in homogeneous solution and the reaction of the redox mediator at the electrode is assumed to be either reversible or mass transport limited. The numerical solution for the non-linear system in the axisymmetrical geometry of the inlaid disc is achieved by using the finite element method in an iterative scheme. The resulting concentration and reaction profiles provide useful insight into the process, and show for some parameter values the formation of an almost spherical, sharp reaction layer or shell, whose position can be predicted approximately. Conditions under which the scheme reverts to the pseudo-first-order EC′ mechanism and expressions to interpret the corresponding current are discussed. A simple approximate expression is worked out under the assumption of constant substrate concentration on the electrode surface. This approximate expression is useful in determining a combination of the parameters from the slope of measurements at low substrate concentration. The effect of some parameters is discussed in detail, leading to two suggestions which can improve the analytical performance: (i) use of the lowest mediator concentration compatible with the background current and (ii) use of the largest microelectrode which reaches steady state in a reasonable time. Experimental measurements using ferrocene monocarboxylic acid as the mediator for the glucose/glucose oxidase system agree with the model and confirm the order of magnitude of the previously reported parameter values.
publishDate 2001
dc.date.none.fl_str_mv 2001
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/S0022-0728(01)00503-4
http://hdl.handle.net/10459.1/48899
http://hdl.handle.net/10459.1/48899
url https://doi.org/10.1016/S0022-0728(01)00503-4
http://hdl.handle.net/10459.1/48899
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/S0022-0728(01)00503-4
Journal of Electroanalytical Chemistry, 2001, vol. 506, num. 2, p. 65-81
dc.rights.none.fl_str_mv (c) Elsevier, 2001
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier, 2001
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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