Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging

Light-sheet microscopes have become the tool of choice for volumetric imaging of large samples. Based on a wide-field acquisition scheme, they are capable of optical sectioning at diffraction-limited resolution and minimal overall photodamage. Unfortunately, traditional architectures are limited in...

Descripción completa

Detalles Bibliográficos
Autores: Zunino, Alessandro, Garzella, Francesco, Trianni, Alberta, Saggau, Peter, Bianchini, Paolo, Diaspro, Alberto, Duocastella, Martí
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/183314
Acceso en línea:https://hdl.handle.net/2445/183314
Access Level:acceso abierto
Palabra clave:Microscopis
Visualització tridimensional
Microscopes
Three-dimensional display systems
id ES_cc856808166e548e12aea20746f737dc
oai_identifier_str oai:recercat.cat:2445/183314
network_acronym_str ES
network_name_str España
repository_id_str
spelling Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D ImagingZunino, AlessandroGarzella, FrancescoTrianni, AlbertaSaggau, PeterBianchini, PaoloDiaspro, AlbertoDuocastella, MartíMicroscopisVisualització tridimensionalMicroscopesThree-dimensional display systemsLight-sheet microscopes have become the tool of choice for volumetric imaging of large samples. Based on a wide-field acquisition scheme, they are capable of optical sectioning at diffraction-limited resolution and minimal overall photodamage. Unfortunately, traditional architectures are limited in speed because 3D images are collected by either sample translation or synchronized movement of both light-sheet and detection objective lens. A promising solution avoiding slow mechanical movements is to extend the depth-of-field of the microscope and moving only the light-sheet. However, this normally comes at the cost of losing light and contrast, compromising the signal-to-noise ratio of the images. Here, we propose an innovative technique devoted to restoring the quality of the images, while preserving the speed of extended depth-of-field microscopes. It is based on generating a stack of parallel light-sheets using a pair of orthogonal acousto-optic deflectors, enabling the simultaneous illumination of different sample planes. Given the extended depth-of-field, all such planes appear in focus and can be acquired in a superimposed single frame. By applying a single-step inversion algorithm, we can decode a stack of frames into a volumetric image whose signal-to-noise ratio and contrast are greatly enhanced. We provide a detailed theoretical framework of the method and demonstrate its feasibility with volumetric images of kidney cell spheroids.American Chemical Society2022202220212022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/183314Articles publicats en revistes (Física Aplicada)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1021/acsphotonics.1c01401ACS Photonics, 2021https://doi.org/10.1021/acsphotonics.1c01401(c) Zunino, Alessandro, et al., 2021info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1833142026-05-29T05:05:01Z
dc.title.none.fl_str_mv Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging
title Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging
spellingShingle Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging
Zunino, Alessandro
Microscopis
Visualització tridimensional
Microscopes
Three-dimensional display systems
title_short Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging
title_full Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging
title_fullStr Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging
title_full_unstemmed Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging
title_sort Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging
dc.creator.none.fl_str_mv Zunino, Alessandro
Garzella, Francesco
Trianni, Alberta
Saggau, Peter
Bianchini, Paolo
Diaspro, Alberto
Duocastella, Martí
author Zunino, Alessandro
author_facet Zunino, Alessandro
Garzella, Francesco
Trianni, Alberta
Saggau, Peter
Bianchini, Paolo
Diaspro, Alberto
Duocastella, Martí
author_role author
author2 Garzella, Francesco
Trianni, Alberta
Saggau, Peter
Bianchini, Paolo
Diaspro, Alberto
Duocastella, Martí
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Microscopis
Visualització tridimensional
Microscopes
Three-dimensional display systems
topic Microscopis
Visualització tridimensional
Microscopes
Three-dimensional display systems
description Light-sheet microscopes have become the tool of choice for volumetric imaging of large samples. Based on a wide-field acquisition scheme, they are capable of optical sectioning at diffraction-limited resolution and minimal overall photodamage. Unfortunately, traditional architectures are limited in speed because 3D images are collected by either sample translation or synchronized movement of both light-sheet and detection objective lens. A promising solution avoiding slow mechanical movements is to extend the depth-of-field of the microscope and moving only the light-sheet. However, this normally comes at the cost of losing light and contrast, compromising the signal-to-noise ratio of the images. Here, we propose an innovative technique devoted to restoring the quality of the images, while preserving the speed of extended depth-of-field microscopes. It is based on generating a stack of parallel light-sheets using a pair of orthogonal acousto-optic deflectors, enabling the simultaneous illumination of different sample planes. Given the extended depth-of-field, all such planes appear in focus and can be acquired in a superimposed single frame. By applying a single-step inversion algorithm, we can decode a stack of frames into a volumetric image whose signal-to-noise ratio and contrast are greatly enhanced. We provide a detailed theoretical framework of the method and demonstrate its feasibility with volumetric images of kidney cell spheroids.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/183314
url https://hdl.handle.net/2445/183314
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1021/acsphotonics.1c01401
ACS Photonics, 2021
https://doi.org/10.1021/acsphotonics.1c01401
dc.rights.none.fl_str_mv (c) Zunino, Alessandro, et al., 2021
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Zunino, Alessandro, et al., 2021
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv Articles publicats en revistes (Física Aplicada)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419727399419904
score 15,81155

Similares en LA Referencia