Serological assays for alveolar and Cystic Echinococcosis-A comparative multi-test study in Switzerland and Kyrgyzstan
27 páginas, 9 tablas
| Autores: | , , , , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/273092 |
| Acceso en línea: | http://hdl.handle.net/10261/273092 https://api.elsevier.com/content/abstract/scopus_id/85129701346 |
| Access Level: | acceso abierto |
| Palabra clave: | ELISA Echinococcus granulosus sensu lato Echinococcus multilocularis Western blot Antibodies Antigens Diagnosis Serology |
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Serological assays for alveolar and Cystic Echinococcosis-A comparative multi-test study in Switzerland and KyrgyzstanKronenberg, Philipp A.Deibel, AnsgarGottstein, BrunoGrimm, FelixMüllhaupt, BeatMeyer Zu Schwabedissen, CordulaAitbaev, SezdbekOmorov, Rakhatbek A.Abdykerimov, Kubanychbek K.Minbaeva, GulnaraUsubalieva, JumagulSiles Lucas, MarPepe, PaolaRinaldi, LauraSpiliotis, MarkusWang, JunhuaMüller, NorbertTorgerson, Paul R.Deplazes, PeterELISAEchinococcus granulosus sensu latoEchinococcus multilocularisWestern blotAntibodiesAntigensDiagnosisSerology27 páginas, 9 tablasBoth alveolar (AE) and cystic echinococcosis (CE) are lacking pathognomonic clinical signs; consequently imaging technologies and serology remain the main pillars for diagnosis. The present study included 100 confirmed treatment-naïve AE and 64 CE patients that were diagnosed in Switzerland or Kyrgyzstan. Overall, 10 native Echinococcus spp. antigens, 3 recombinant antigens, and 4 commercial assays were comparatively evaluated. All native E. multilocularis antigens were produced in duplicates with a European and a Kyrgyz isolate and showed identical test values for the diagnosis of AE and CE. Native antigens and three commercial tests showed high diagnostic sensitivities (Se: 86-96%) and specificities (Sp: 96-99%) for the diagnosis of AE and CE in Swiss patients. In Kyrgyz patients, values of sensitivities and specificities were 10-20% lower as compared to the Swiss patients' findings. For the sero-diagnosis of AE in Kyrgyzstan, a test-combination of an E. multilocularis protoscolex antigen and the recombinant antigen Em95 appears to be the most suitable test strategy (Se: 98%, Sp: 87%). For the diagnosis of CE in both countries, test performances were hampered by major cross-reactions with AE patients and other parasitic diseases as well as by limited diagnostic sensitivities (93% in Switzerland and 76% in Kyrgyzstan, respectively).This research was partially funded by the Swiss National Science Foundation, grantnumber: 173131-“Transmission modelling of emergent echinococcosis in Kyrgyzstan” and by theproject “New sustainable tools and innovative actions to control cystic ECHINOcoccosis in sheepfarms in the MEDiterranean area: improvement of diagnosis and SAFEty in response to climaticchanges-ECHINO-SAFE-MED”, supported by PRIMA (Partnernship for research and innovation inthe Mediterranean areaPeer reviewedMultidisciplinary Digital Publishing InstituteSwiss National Science FoundationSiles Lucas, Mar [0000-0002-1257-2562]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/273092https://api.elsevier.com/content/abstract/scopus_id/85129701346reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésPathogens (Basel, Switzerland)http://dx.doi.org/10.3390/pathogens11050518Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2730922026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Serological assays for alveolar and Cystic Echinococcosis-A comparative multi-test study in Switzerland and Kyrgyzstan |
| title |
Serological assays for alveolar and Cystic Echinococcosis-A comparative multi-test study in Switzerland and Kyrgyzstan |
| spellingShingle |
Serological assays for alveolar and Cystic Echinococcosis-A comparative multi-test study in Switzerland and Kyrgyzstan Kronenberg, Philipp A. ELISA Echinococcus granulosus sensu lato Echinococcus multilocularis Western blot Antibodies Antigens Diagnosis Serology |
| title_short |
Serological assays for alveolar and Cystic Echinococcosis-A comparative multi-test study in Switzerland and Kyrgyzstan |
| title_full |
Serological assays for alveolar and Cystic Echinococcosis-A comparative multi-test study in Switzerland and Kyrgyzstan |
| title_fullStr |
Serological assays for alveolar and Cystic Echinococcosis-A comparative multi-test study in Switzerland and Kyrgyzstan |
| title_full_unstemmed |
Serological assays for alveolar and Cystic Echinococcosis-A comparative multi-test study in Switzerland and Kyrgyzstan |
| title_sort |
Serological assays for alveolar and Cystic Echinococcosis-A comparative multi-test study in Switzerland and Kyrgyzstan |
| dc.creator.none.fl_str_mv |
Kronenberg, Philipp A. Deibel, Ansgar Gottstein, Bruno Grimm, Felix Müllhaupt, Beat Meyer Zu Schwabedissen, Cordula Aitbaev, Sezdbek Omorov, Rakhatbek A. Abdykerimov, Kubanychbek K. Minbaeva, Gulnara Usubalieva, Jumagul Siles Lucas, Mar Pepe, Paola Rinaldi, Laura Spiliotis, Markus Wang, Junhua Müller, Norbert Torgerson, Paul R. Deplazes, Peter |
| author |
Kronenberg, Philipp A. |
| author_facet |
Kronenberg, Philipp A. Deibel, Ansgar Gottstein, Bruno Grimm, Felix Müllhaupt, Beat Meyer Zu Schwabedissen, Cordula Aitbaev, Sezdbek Omorov, Rakhatbek A. Abdykerimov, Kubanychbek K. Minbaeva, Gulnara Usubalieva, Jumagul Siles Lucas, Mar Pepe, Paola Rinaldi, Laura Spiliotis, Markus Wang, Junhua Müller, Norbert Torgerson, Paul R. Deplazes, Peter |
| author_role |
author |
| author2 |
Deibel, Ansgar Gottstein, Bruno Grimm, Felix Müllhaupt, Beat Meyer Zu Schwabedissen, Cordula Aitbaev, Sezdbek Omorov, Rakhatbek A. Abdykerimov, Kubanychbek K. Minbaeva, Gulnara Usubalieva, Jumagul Siles Lucas, Mar Pepe, Paola Rinaldi, Laura Spiliotis, Markus Wang, Junhua Müller, Norbert Torgerson, Paul R. Deplazes, Peter |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Swiss National Science Foundation Siles Lucas, Mar [0000-0002-1257-2562] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
ELISA Echinococcus granulosus sensu lato Echinococcus multilocularis Western blot Antibodies Antigens Diagnosis Serology |
| topic |
ELISA Echinococcus granulosus sensu lato Echinococcus multilocularis Western blot Antibodies Antigens Diagnosis Serology |
| description |
27 páginas, 9 tablas |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/273092 https://api.elsevier.com/content/abstract/scopus_id/85129701346 |
| url |
http://hdl.handle.net/10261/273092 https://api.elsevier.com/content/abstract/scopus_id/85129701346 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Pathogens (Basel, Switzerland) http://dx.doi.org/10.3390/pathogens11050518 Sí |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
| publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
| dc.source.none.fl_str_mv |
reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869419718009421824 |
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15,812429 |