Standardization procedure for flow cytometry data harmonization in prospective multicenter studies

One of the most challenging objective for clinical cytometry in prospective multicenter immunomonitoring trials is to compare frequencies, absolute numbers of leukocyte populations and further the mean fluorescence intensities of cell markers, especially when the data are generated from different in...

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Bibliographic Details
Authors: Le Lann, Lucas, Jouve, Pierre Emmanuel, Alarcón Riquelme, Marta, Jamin, Christophe, Pers, Jacques-Olivier, PRECISESADS Flow Cytometry Study Group, PRECISESADS Clinical Consortium, Cervera i Segura, Ricard, 1960-
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/177015
Online Access:https://hdl.handle.net/2445/177015
Access Level:Open access
Keyword:Citometria de fluxe
Leucòcits
Flow cytometry
Leucocytes
Description
Summary:One of the most challenging objective for clinical cytometry in prospective multicenter immunomonitoring trials is to compare frequencies, absolute numbers of leukocyte populations and further the mean fluorescence intensities of cell markers, especially when the data are generated from different instruments. Here, we describe an innovative standardization workflow to compare all data to carry out any large-scale, prospective multicentric flow cytometry analysis whatever the duration, the number or type of instruments required for the realization of such projects.