The imperative for quality control programs in Monkeypox virus DNA testing by PCR: CIBERINFEC quality control
Monkeypox; PCR; Quality control
| Autores: | , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2023 |
| País: | España |
| Recursos: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositório: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:11351/10809 |
| Acesso em linha: | https://hdl.handle.net/11351/10809 http://hdl.handle.net/11351/10809 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Control de qualitat Verola del mico - Diagnòstic ADN ORGANISMS::Viruses::DNA Viruses::Poxviridae::Chordopoxvirinae::Orthopoxvirus::Monkeypox virus CHEMICALS AND DRUGS::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA PUBLIC HEALTH::Environmental Health::General Aspects::Control::Quality Control ORGANISMOS::virus::virus ADN::Poxviridae::Chordopoxvirinae::Orthopoxvirus::virus de la viruela del mono COMPUESTOS QUÍMICOS Y DROGAS::nucleótidos y nucleósidos de ácidos nucleicos::ácidos nucleicos::ADN SALUD PÚBLICA::salud ambiental::aspectos generales::control::control de calidad |
| id |
ES_cbdde495aab295199452ed9d7ee6e5cc |
|---|---|
| oai_identifier_str |
oai:recercat.cat:11351/10809 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| dc.title.none.fl_str_mv |
The imperative for quality control programs in Monkeypox virus DNA testing by PCR: CIBERINFEC quality control |
| title |
The imperative for quality control programs in Monkeypox virus DNA testing by PCR: CIBERINFEC quality control |
| spellingShingle |
The imperative for quality control programs in Monkeypox virus DNA testing by PCR: CIBERINFEC quality control de Salazar, Adolfo Control de qualitat Verola del mico - Diagnòstic ADN ORGANISMS::Viruses::DNA Viruses::Poxviridae::Chordopoxvirinae::Orthopoxvirus::Monkeypox virus CHEMICALS AND DRUGS::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA PUBLIC HEALTH::Environmental Health::General Aspects::Control::Quality Control ORGANISMOS::virus::virus ADN::Poxviridae::Chordopoxvirinae::Orthopoxvirus::virus de la viruela del mono COMPUESTOS QUÍMICOS Y DROGAS::nucleótidos y nucleósidos de ácidos nucleicos::ácidos nucleicos::ADN SALUD PÚBLICA::salud ambiental::aspectos generales::control::control de calidad |
| title_short |
The imperative for quality control programs in Monkeypox virus DNA testing by PCR: CIBERINFEC quality control |
| title_full |
The imperative for quality control programs in Monkeypox virus DNA testing by PCR: CIBERINFEC quality control |
| title_fullStr |
The imperative for quality control programs in Monkeypox virus DNA testing by PCR: CIBERINFEC quality control |
| title_full_unstemmed |
The imperative for quality control programs in Monkeypox virus DNA testing by PCR: CIBERINFEC quality control |
| title_sort |
The imperative for quality control programs in Monkeypox virus DNA testing by PCR: CIBERINFEC quality control |
| dc.creator.none.fl_str_mv |
de Salazar, Adolfo Martínez, Miguel J. Navero Castillejos, Jessica Negredo, Anabel Galan, Juan Carlos Rojo Molinero, Estrella Antón Pagarolas, Andrés |
| author |
de Salazar, Adolfo |
| author_facet |
de Salazar, Adolfo Martínez, Miguel J. Navero Castillejos, Jessica Negredo, Anabel Galan, Juan Carlos Rojo Molinero, Estrella Antón Pagarolas, Andrés |
| author_role |
author |
| author2 |
Martínez, Miguel J. Navero Castillejos, Jessica Negredo, Anabel Galan, Juan Carlos Rojo Molinero, Estrella Antón Pagarolas, Andrés |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Institut Català de la Salut [de Salazar A] Servicio de Microbiología, Hospital Universitario San Cecilio, Granada, Spain. Instituto de Investigación Biosanitaria Ibs.Granada, Granada, Spain. Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain. [Martínez MJ] Servicio de Microbiología, Hospital Clinic de Barcelona, Spain. Instituto de Salud Global de Barcelona (ISGloba), Barcelona, Spain. [Navero‐Castillejos J] Servicio de Microbiología, Hospital Clinic de Barcelona, Spain. [Negredo A] Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain. Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. [Galán JC] Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain. Centro de Investigación Biomédica en Red en Epidemiología y Salud Pública (CIBERESP), Madrid, Spain. [Rojo Molinero E] Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain. Departamento de Microbiología, Hospital Universitario Son Espases, Health Research Institute of Balearic Islands (IdISBa), Palma, Spain. [Antón Pagarolas A] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. PROSICS Barcelona, Universitat Autònoma de Barcelona, Bellaterra, Spain Vall d'Hebron Barcelona Hospital Campus |
| dc.subject.none.fl_str_mv |
Control de qualitat Verola del mico - Diagnòstic ADN ORGANISMS::Viruses::DNA Viruses::Poxviridae::Chordopoxvirinae::Orthopoxvirus::Monkeypox virus CHEMICALS AND DRUGS::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA PUBLIC HEALTH::Environmental Health::General Aspects::Control::Quality Control ORGANISMOS::virus::virus ADN::Poxviridae::Chordopoxvirinae::Orthopoxvirus::virus de la viruela del mono COMPUESTOS QUÍMICOS Y DROGAS::nucleótidos y nucleósidos de ácidos nucleicos::ácidos nucleicos::ADN SALUD PÚBLICA::salud ambiental::aspectos generales::control::control de calidad |
| topic |
Control de qualitat Verola del mico - Diagnòstic ADN ORGANISMS::Viruses::DNA Viruses::Poxviridae::Chordopoxvirinae::Orthopoxvirus::Monkeypox virus CHEMICALS AND DRUGS::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA PUBLIC HEALTH::Environmental Health::General Aspects::Control::Quality Control ORGANISMOS::virus::virus ADN::Poxviridae::Chordopoxvirinae::Orthopoxvirus::virus de la viruela del mono COMPUESTOS QUÍMICOS Y DROGAS::nucleótidos y nucleósidos de ácidos nucleicos::ácidos nucleicos::ADN SALUD PÚBLICA::salud ambiental::aspectos generales::control::control de calidad |
| description |
Monkeypox; PCR; Quality control |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2024 2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/11351/10809 http://hdl.handle.net/11351/10809 |
| url |
https://hdl.handle.net/11351/10809 http://hdl.handle.net/11351/10809 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Journal of Medical Virology;95(11) https://doi.org/10.1002/jmv.29240 |
| dc.rights.none.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley |
| publisher.none.fl_str_mv |
Wiley |
| dc.source.none.fl_str_mv |
Scientia reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str |
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| reponame_str |
Recercat. Dipósit de la Recerca de Catalunya |
| collection |
Recercat. Dipósit de la Recerca de Catalunya |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869419625770385408 |
| spelling |
The imperative for quality control programs in Monkeypox virus DNA testing by PCR: CIBERINFEC quality controlde Salazar, AdolfoMartínez, Miguel J.Navero Castillejos, JessicaNegredo, AnabelGalan, Juan CarlosRojo Molinero, EstrellaAntón Pagarolas, AndrésControl de qualitatVerola del mico - DiagnòsticADNORGANISMS::Viruses::DNA Viruses::Poxviridae::Chordopoxvirinae::Orthopoxvirus::Monkeypox virusCHEMICALS AND DRUGS::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNAPUBLIC HEALTH::Environmental Health::General Aspects::Control::Quality ControlORGANISMOS::virus::virus ADN::Poxviridae::Chordopoxvirinae::Orthopoxvirus::virus de la viruela del monoCOMPUESTOS QUÍMICOS Y DROGAS::nucleótidos y nucleósidos de ácidos nucleicos::ácidos nucleicos::ADNSALUD PÚBLICA::salud ambiental::aspectos generales::control::control de calidadMonkeypox; PCR; Quality controlVerola del mico; PCR; Control de qualitatViruela del mono; PCR; Control de calidadTo evaluate molecular assays for Mpox diagnosis available in various clinical microbiology services in Spain through a quality control (QC) approach. A total of 14 centers from across Spain participated in the study. The Reference Laboratory dispatched eight serum samples and eight nucleic acid extracts to each participating center. Some samples were spiked with Mpox or Vaccinia virus to mimic positive samples for Mpox or other orthopox viruses. Participating centers provided information on the results obtained, as well as the laboratory methods used. Among the 14 participating centers seven different commercial assays were employed, with the most commonly used kit being LightMix Modular Orthopox/Monkeypox (Mpox) Virus (Roche®). Of the 12 centers conducting Mpox determinations, concordance ranged from 62.5% (n = 1) to 100% (n = 11) for eluates and from 75.0% (n = 1) to 100% (n = 10) for serum. Among the 10 centers performing Orthopoxvirus determinations, a 100% concordance was observed for eluates, while for serum, concordance ranged from 87.5% (n = 6) to 100% (n = 4). Repeatedly, 6 different centers reported a false negative in serum samples for Orthopoxvirus diagnosis, particularly in a sample with borderline Ct = 39. Conversely, one center, using the TaqMan™ Mpox Virus Microbe Detection Assay (Thermo Fisher), reported false positives in Mpox diagnosis for samples spiked with vaccinia virus due to cross-reactions. We observed a positive correlation of various diagnostic assays for Mpox used by the participating centers with the reference values. Our results highlight the significance of standardization, validation, and ongoing QC in the microbiological diagnosis of infectious diseases, which might be particularly relevant for emerging viruses.This research was supported by CIBER (Strategic Action for Monkeypox)—Consorcio Centro de Investigación Biomédica en Red—(CB 2021), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea—NextGenerationEU. A. d. S. is supported by ‘Instituto de Salud Carlos III’ (grant number JR22/00055).WileyInstitut Català de la Salut[de Salazar A] Servicio de Microbiología, Hospital Universitario San Cecilio, Granada, Spain. Instituto de Investigación Biosanitaria Ibs.Granada, Granada, Spain. Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain. [Martínez MJ] Servicio de Microbiología, Hospital Clinic de Barcelona, Spain. Instituto de Salud Global de Barcelona (ISGloba), Barcelona, Spain. [Navero‐Castillejos J] Servicio de Microbiología, Hospital Clinic de Barcelona, Spain. [Negredo A] Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain. Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. [Galán JC] Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain. Centro de Investigación Biomédica en Red en Epidemiología y Salud Pública (CIBERESP), Madrid, Spain. [Rojo Molinero E] Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain. Departamento de Microbiología, Hospital Universitario Son Espases, Health Research Institute of Balearic Islands (IdISBa), Palma, Spain. [Antón Pagarolas A] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. PROSICS Barcelona, Universitat Autònoma de Barcelona, Bellaterra, SpainVall d'Hebron Barcelona Hospital Campus202420242023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/11351/10809http://hdl.handle.net/11351/10809Scientiareponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésJournal of Medical Virology;95(11)https://doi.org/10.1002/jmv.29240Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:11351/108092026-05-29T05:05:01Z |
| score |
15,198674 |