Modelling Neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents

Neuron-microglia co-cultures treated with pro-inflammatory agents are a useful tool to study neuroinflammation in vitro, where to test the potential neuroprotective effect of anti-inflammatory compounds. However, a great diversity of experimental conditions can be found in the literature, making dif...

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Autores: Gresa Arribas, Nuria, Viéitez, Cristina, Dentesano, Guido, Serratosa i Serdà, Joan, Saura Martí, Josep, Solà i Subirana, Carme
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/48023
Acceso en línea:https://hdl.handle.net/2445/48023
Access Level:acceso abierto
Palabra clave:Micròglia
Neurotoxicologia
Malalties neurodegeneratives
Terapèutica
Microglia
Neurotoxicology
Neurodegenerative Diseases
Therapeutics
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spelling Modelling Neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agentsGresa Arribas, NuriaViéitez, CristinaDentesano, GuidoSerratosa i Serdà, JoanSaura Martí, JosepSolà i Subirana, CarmeMicrògliaNeurotoxicologiaMalalties neurodegenerativesTerapèuticaMicrogliaNeurotoxicologyNeurodegenerative DiseasesTherapeuticsNeuron-microglia co-cultures treated with pro-inflammatory agents are a useful tool to study neuroinflammation in vitro, where to test the potential neuroprotective effect of anti-inflammatory compounds. However, a great diversity of experimental conditions can be found in the literature, making difficult to select the working conditions when considering this approach for the first time. We compared the use of neuron-primary microglia and neuron-BV2 cells (a microglial cell line) co-cultures, using different neuron:microglia ratios, treatments and time post-treatment to induce glial activation and derived neurotoxicity. We show that each model requires different experimental conditions, but that both neuron-BV2 and neuron-primary microglia LPS/IFN-γ-treated co-cultures are good to study the potential neuroprotective effect of anti-inflammatory agents. The contribution of different pro-inflammatory parameters in the neurotoxicity induced by reactive microglial cells was determined. IL-10 pre-treatment completely inhibited LPS/IFN-γ-induced TNF-α and IL-6 release, and COX-2 expression both in BV2 and primary microglial cultures, but not NO production and iNOS expression. However, LPS/IFN-γ induced neurotoxicity was not inhibited in IL-10 pre-treated co-cultures. The inhibition of NO production using the specific iNOS inhibitor 1400 W totally abolished the neurotoxic effect of LPS/IFN-γ, suggesting a major role for NO in the neurotoxic effect of activated microglia. Consequently, among the anti-inflammatory agents, special attention should be paid to compounds that inhibit NO production.Public Library of Science (PLoS)2013201320122013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/48023Articles publicats en revistes (Ciències Fisiològiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0045227PLoS One, 2012, vol. 7, num. 9, p. e45227http://dx.doi.org/10.1371/journal.pone.0045227cc-by (c) Gresa Arribas et al., 2012http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/480232026-05-29T05:05:01Z
dc.title.none.fl_str_mv Modelling Neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents
title Modelling Neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents
spellingShingle Modelling Neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents
Gresa Arribas, Nuria
Micròglia
Neurotoxicologia
Malalties neurodegeneratives
Terapèutica
Microglia
Neurotoxicology
Neurodegenerative Diseases
Therapeutics
title_short Modelling Neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents
title_full Modelling Neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents
title_fullStr Modelling Neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents
title_full_unstemmed Modelling Neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents
title_sort Modelling Neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents
dc.creator.none.fl_str_mv Gresa Arribas, Nuria
Viéitez, Cristina
Dentesano, Guido
Serratosa i Serdà, Joan
Saura Martí, Josep
Solà i Subirana, Carme
author Gresa Arribas, Nuria
author_facet Gresa Arribas, Nuria
Viéitez, Cristina
Dentesano, Guido
Serratosa i Serdà, Joan
Saura Martí, Josep
Solà i Subirana, Carme
author_role author
author2 Viéitez, Cristina
Dentesano, Guido
Serratosa i Serdà, Joan
Saura Martí, Josep
Solà i Subirana, Carme
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Micròglia
Neurotoxicologia
Malalties neurodegeneratives
Terapèutica
Microglia
Neurotoxicology
Neurodegenerative Diseases
Therapeutics
topic Micròglia
Neurotoxicologia
Malalties neurodegeneratives
Terapèutica
Microglia
Neurotoxicology
Neurodegenerative Diseases
Therapeutics
description Neuron-microglia co-cultures treated with pro-inflammatory agents are a useful tool to study neuroinflammation in vitro, where to test the potential neuroprotective effect of anti-inflammatory compounds. However, a great diversity of experimental conditions can be found in the literature, making difficult to select the working conditions when considering this approach for the first time. We compared the use of neuron-primary microglia and neuron-BV2 cells (a microglial cell line) co-cultures, using different neuron:microglia ratios, treatments and time post-treatment to induce glial activation and derived neurotoxicity. We show that each model requires different experimental conditions, but that both neuron-BV2 and neuron-primary microglia LPS/IFN-γ-treated co-cultures are good to study the potential neuroprotective effect of anti-inflammatory agents. The contribution of different pro-inflammatory parameters in the neurotoxicity induced by reactive microglial cells was determined. IL-10 pre-treatment completely inhibited LPS/IFN-γ-induced TNF-α and IL-6 release, and COX-2 expression both in BV2 and primary microglial cultures, but not NO production and iNOS expression. However, LPS/IFN-γ induced neurotoxicity was not inhibited in IL-10 pre-treated co-cultures. The inhibition of NO production using the specific iNOS inhibitor 1400 W totally abolished the neurotoxic effect of LPS/IFN-γ, suggesting a major role for NO in the neurotoxic effect of activated microglia. Consequently, among the anti-inflammatory agents, special attention should be paid to compounds that inhibit NO production.
publishDate 2012
dc.date.none.fl_str_mv 2012
2013
2013
2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/48023
url https://hdl.handle.net/2445/48023
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0045227
PLoS One, 2012, vol. 7, num. 9, p. e45227
http://dx.doi.org/10.1371/journal.pone.0045227
dc.rights.none.fl_str_mv cc-by (c) Gresa Arribas et al., 2012
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Gresa Arribas et al., 2012
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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