Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in Rats

Propyl-propanethiosulfinate (PTS) is a component of Allium essential oils. This organosulfur molecule can be used as a feed additive to decrease the appearance of bacterial resistances caused by the residues of antibiotics. In previous in vitro genotoxicity studies, contradictory results were report...

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Autores: Cascajosa Lira, Antonio, Puerto Rodríguez, María, Prieto Ortega, Ana Isabel, Pichardo Sánchez, Silvia, Díez-Quijada Jiménez, Leticia, Baños, Alberto, Gillamón, Enrique, Moyano, Rosario, Molina Hernández, Verónica, Jos Gallego, Ángeles Mencía, Cameán Fernández, Ana María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/114978
Acceso en línea:https://hdl.handle.net/11441/114978
https://doi.org/10.3390/foods10050989
Access Level:acceso abierto
Palabra clave:Micronucleus
Comet assay
Genotoxicity
Organosulfur compounds
Propyl-propanethio sulfinate
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spelling Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in RatsCascajosa Lira, AntonioPuerto Rodríguez, MaríaPrieto Ortega, Ana IsabelPichardo Sánchez, SilviaDíez-Quijada Jiménez, LeticiaBaños, AlbertoGillamón, EnriqueMoyano, RosarioMolina Hernández, VerónicaJos Gallego, Ángeles MencíaCameán Fernández, Ana MaríaMicronucleusComet assayGenotoxicityOrganosulfur compoundsPropyl-propanethio sulfinatePropyl-propanethiosulfinate (PTS) is a component of Allium essential oils. This organosulfur molecule can be used as a feed additive to decrease the appearance of bacterial resistances caused by the residues of antibiotics. In previous in vitro genotoxicity studies, contradictory results were reported for PTS. In this work, the in vivo genotoxicity of PTS in male and female rats was assessed for the first time, following OECD (Organisation for Economic Co-operation and Development) guidelines. After oral administration (doses: 5.5, 17.4, and 55.0 mg/kg PTS body weight), a combination of the micronucleus (MN) assay (OECD 474) in bone marrow and the standard and enzyme-modified comet assay (OECD 489) was performed. After necropsy, histopathological studies were also carried out. The results did not show the in vivo genotoxicity of PTS at any doses assayed, revealed by the absence of increased MN, and DNA strand breaks or oxidative DNA damage in the standard and enzyme-modified comet assays. The histopathological study revealed that only the highest dose tested (55.0 mg/kg) in the liver and all dose groups in the stomach presented minimal pathological lesions in the organs studied. Consequently, the present work confirms that PTS is not genotoxic at the doses assayed, and it is a promising natural alternative to synthetic preservatives and antibiotics in animal feed.Ministerio de Ciencia e Innovación RTC-2017-6199- 2Junta de Andalucía AT 2017-5323, P18-TP-2147Multidisciplinary Digital Publishing Institute (MDPI)Nutrición y Bromatología, Toxicología y Medicina Legal2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/114978https://doi.org/10.3390/foods10050989reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésFoods, 10 (5), 989.RTC-2017-6199- 2AT 2017-5323P18-TP-2147https://doi.org/10.3390/foods10050989info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1149782026-06-17T12:51:07Z
dc.title.none.fl_str_mv Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in Rats
title Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in Rats
spellingShingle Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in Rats
Cascajosa Lira, Antonio
Micronucleus
Comet assay
Genotoxicity
Organosulfur compounds
Propyl-propanethio sulfinate
title_short Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in Rats
title_full Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in Rats
title_fullStr Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in Rats
title_full_unstemmed Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in Rats
title_sort Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in Rats
dc.creator.none.fl_str_mv Cascajosa Lira, Antonio
Puerto Rodríguez, María
Prieto Ortega, Ana Isabel
Pichardo Sánchez, Silvia
Díez-Quijada Jiménez, Leticia
Baños, Alberto
Gillamón, Enrique
Moyano, Rosario
Molina Hernández, Verónica
Jos Gallego, Ángeles Mencía
Cameán Fernández, Ana María
author Cascajosa Lira, Antonio
author_facet Cascajosa Lira, Antonio
Puerto Rodríguez, María
Prieto Ortega, Ana Isabel
Pichardo Sánchez, Silvia
Díez-Quijada Jiménez, Leticia
Baños, Alberto
Gillamón, Enrique
Moyano, Rosario
Molina Hernández, Verónica
Jos Gallego, Ángeles Mencía
Cameán Fernández, Ana María
author_role author
author2 Puerto Rodríguez, María
Prieto Ortega, Ana Isabel
Pichardo Sánchez, Silvia
Díez-Quijada Jiménez, Leticia
Baños, Alberto
Gillamón, Enrique
Moyano, Rosario
Molina Hernández, Verónica
Jos Gallego, Ángeles Mencía
Cameán Fernández, Ana María
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Nutrición y Bromatología, Toxicología y Medicina Legal
dc.subject.none.fl_str_mv Micronucleus
Comet assay
Genotoxicity
Organosulfur compounds
Propyl-propanethio sulfinate
topic Micronucleus
Comet assay
Genotoxicity
Organosulfur compounds
Propyl-propanethio sulfinate
description Propyl-propanethiosulfinate (PTS) is a component of Allium essential oils. This organosulfur molecule can be used as a feed additive to decrease the appearance of bacterial resistances caused by the residues of antibiotics. In previous in vitro genotoxicity studies, contradictory results were reported for PTS. In this work, the in vivo genotoxicity of PTS in male and female rats was assessed for the first time, following OECD (Organisation for Economic Co-operation and Development) guidelines. After oral administration (doses: 5.5, 17.4, and 55.0 mg/kg PTS body weight), a combination of the micronucleus (MN) assay (OECD 474) in bone marrow and the standard and enzyme-modified comet assay (OECD 489) was performed. After necropsy, histopathological studies were also carried out. The results did not show the in vivo genotoxicity of PTS at any doses assayed, revealed by the absence of increased MN, and DNA strand breaks or oxidative DNA damage in the standard and enzyme-modified comet assays. The histopathological study revealed that only the highest dose tested (55.0 mg/kg) in the liver and all dose groups in the stomach presented minimal pathological lesions in the organs studied. Consequently, the present work confirms that PTS is not genotoxic at the doses assayed, and it is a promising natural alternative to synthetic preservatives and antibiotics in animal feed.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/114978
https://doi.org/10.3390/foods10050989
url https://hdl.handle.net/11441/114978
https://doi.org/10.3390/foods10050989
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Foods, 10 (5), 989.
RTC-2017-6199- 2
AT 2017-5323
P18-TP-2147
https://doi.org/10.3390/foods10050989
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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