Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector

Induced pluripotent stem (iPS) cells have generated keen interest/ndue to their potential use in regenerative medicine. They have/nbeen obtained from various cell types of both mice and humans by/nexogenous delivery of different combinations of Oct4, Sox2, Klf4,/nc-Myc, Nanog, and Lin28. The deliver...

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Autores: Rodríguez Pizà, Ignasi, Barragan, Montserrat, González, Federico, Izpisúa Belmonte, J. C., Batlle Morera, Laura, Vassena, Rita, Montserrat Pulido, Núria, Tiscornia, Gustavo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/12427
Acceso en línea:http://hdl.handle.net/10230/12427
http://dx.doi.org/10.1073/pnas.0901471106
Access Level:acceso abierto
Palabra clave:Medicina regenerativa
Cél·lules mare embrionàries
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spelling Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vectorRodríguez Pizà, IgnasiBarragan, MontserratGonzález, FedericoIzpisúa Belmonte, J. C.Batlle Morera, LauraVassena, RitaMontserrat Pulido, NúriaTiscornia, GustavoMedicina regenerativaCél·lules mare embrionàriesInduced pluripotent stem (iPS) cells have generated keen interest/ndue to their potential use in regenerative medicine. They have/nbeen obtained from various cell types of both mice and humans by/nexogenous delivery of different combinations of Oct4, Sox2, Klf4,/nc-Myc, Nanog, and Lin28. The delivery of these transcription factors/nhas mostly entailed the use of integrating viral vectors (retroviruses/nor lentiviruses), carrying the risk of both insertional mutagenesis/nand oncogenesis due to misexpression of these exogenous/nfactors. Therefore, obtaining iPS cells that do not carry integrated/ntransgene sequences is an important prerequisite for their eventual/ntherapeutic use. Here we report the generation of iPS cell lines/nfrom mouse embryonic fibroblasts with no evidence of integration/nof the reprogramming vector in their genome, achieved by nucleofection/nof a polycistronic construct coexpressing Oct4, Sox2, Klf4,/nand c-MycNational Academy of Sciences201120112009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/12427http://dx.doi.org/10.1073/pnas.0901471106reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésProceedings of the National Academy of Sciences of the United States of America. 2009;106(22):8918-22© National Academy of Sciencesinfo:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/124272026-06-12T07:21:37Z
dc.title.none.fl_str_mv Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
title Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
spellingShingle Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
Rodríguez Pizà, Ignasi
Medicina regenerativa
Cél·lules mare embrionàries
title_short Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
title_full Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
title_fullStr Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
title_full_unstemmed Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
title_sort Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
dc.creator.none.fl_str_mv Rodríguez Pizà, Ignasi
Barragan, Montserrat
González, Federico
Izpisúa Belmonte, J. C.
Batlle Morera, Laura
Vassena, Rita
Montserrat Pulido, Núria
Tiscornia, Gustavo
author Rodríguez Pizà, Ignasi
author_facet Rodríguez Pizà, Ignasi
Barragan, Montserrat
González, Federico
Izpisúa Belmonte, J. C.
Batlle Morera, Laura
Vassena, Rita
Montserrat Pulido, Núria
Tiscornia, Gustavo
author_role author
author2 Barragan, Montserrat
González, Federico
Izpisúa Belmonte, J. C.
Batlle Morera, Laura
Vassena, Rita
Montserrat Pulido, Núria
Tiscornia, Gustavo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicina regenerativa
Cél·lules mare embrionàries
topic Medicina regenerativa
Cél·lules mare embrionàries
description Induced pluripotent stem (iPS) cells have generated keen interest/ndue to their potential use in regenerative medicine. They have/nbeen obtained from various cell types of both mice and humans by/nexogenous delivery of different combinations of Oct4, Sox2, Klf4,/nc-Myc, Nanog, and Lin28. The delivery of these transcription factors/nhas mostly entailed the use of integrating viral vectors (retroviruses/nor lentiviruses), carrying the risk of both insertional mutagenesis/nand oncogenesis due to misexpression of these exogenous/nfactors. Therefore, obtaining iPS cells that do not carry integrated/ntransgene sequences is an important prerequisite for their eventual/ntherapeutic use. Here we report the generation of iPS cell lines/nfrom mouse embryonic fibroblasts with no evidence of integration/nof the reprogramming vector in their genome, achieved by nucleofection/nof a polycistronic construct coexpressing Oct4, Sox2, Klf4,/nand c-Myc
publishDate 2009
dc.date.none.fl_str_mv 2009
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/12427
http://dx.doi.org/10.1073/pnas.0901471106
url http://hdl.handle.net/10230/12427
http://dx.doi.org/10.1073/pnas.0901471106
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Proceedings of the National Academy of Sciences of the United States of America. 2009;106(22):8918-22
dc.rights.none.fl_str_mv © National Academy of Sciences
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © National Academy of Sciences
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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