Overexpression of corticotropin-releasing factor in intestinal mucosal eosinophils is associated with clinical severity in Diarrhea-Predominant Irritable Bowel Syndrome
Corticotropin-releasing factor (CRF) has been identified in intestinal mucosal eosinophils and associated with psychological stress and gut dysfunction. Irritable bowel syndrome (IBS) is commonly characterized by altered intestinal motility, immune activation, and increased gut barrier permeability...
| Autores: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:253129 |
| Acceso en línea: | https://ddd.uab.cat/record/253129 https://dx.doi.org/urn:doi:10.1038/s41598-020-77176-x |
| Access Level: | acceso abierto |
| Palabra clave: | Irritable bowel syndrome Mucosal immunology |
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oai:ddd.uab.cat:253129 |
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España |
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| dc.title.none.fl_str_mv |
Overexpression of corticotropin-releasing factor in intestinal mucosal eosinophils is associated with clinical severity in Diarrhea-Predominant Irritable Bowel Syndrome |
| title |
Overexpression of corticotropin-releasing factor in intestinal mucosal eosinophils is associated with clinical severity in Diarrhea-Predominant Irritable Bowel Syndrome |
| spellingShingle |
Overexpression of corticotropin-releasing factor in intestinal mucosal eosinophils is associated with clinical severity in Diarrhea-Predominant Irritable Bowel Syndrome Salvo-Romero, Eloísa Irritable bowel syndrome Mucosal immunology |
| title_short |
Overexpression of corticotropin-releasing factor in intestinal mucosal eosinophils is associated with clinical severity in Diarrhea-Predominant Irritable Bowel Syndrome |
| title_full |
Overexpression of corticotropin-releasing factor in intestinal mucosal eosinophils is associated with clinical severity in Diarrhea-Predominant Irritable Bowel Syndrome |
| title_fullStr |
Overexpression of corticotropin-releasing factor in intestinal mucosal eosinophils is associated with clinical severity in Diarrhea-Predominant Irritable Bowel Syndrome |
| title_full_unstemmed |
Overexpression of corticotropin-releasing factor in intestinal mucosal eosinophils is associated with clinical severity in Diarrhea-Predominant Irritable Bowel Syndrome |
| title_sort |
Overexpression of corticotropin-releasing factor in intestinal mucosal eosinophils is associated with clinical severity in Diarrhea-Predominant Irritable Bowel Syndrome |
| dc.creator.none.fl_str_mv |
Salvo-Romero, Eloísa Martínez, Cristina|||0000-0002-9368-1356 Lobo Álvarez, Beatriz|||0000-0003-3391-7125 Rodiño-Janeiro, Bruno K.|||0000-0002-0633-6774 Pigrau Pastor, Marc|||0000-0001-6801-3017 Sánchez Chardi, Alejandro|||0000-0002-8789-1883 González Castro, Ana Maria|||0000-0003-3955-3318 Fortea, Marina|||0000-0001-8885-7495 Pardo-Camacho, Cristina Nieto Ruiz, Adoración|||0000-0002-3421-2840 Expósito, Elba|||0000-0001-6088-3149 Guagnozzi, Danila|||0000-0002-6171-1901 Rodríguez-Urrutia, Amanda|||0000-0001-6882-205X de Torres, Inés|||0000-0002-5495-9140 Farré, Ricard|||0000-0001-7158-171X Azpiroz Vidaur, Fernando|||0000-0002-7327-960X Alonso Cotoner, Carmen|||0000-0002-3483-2919 Santos, Javier|||0000-0002-4798-5033 Vicario Perez, Maria|||0000-0001-9622-3185 |
| author |
Salvo-Romero, Eloísa |
| author_facet |
Salvo-Romero, Eloísa Martínez, Cristina|||0000-0002-9368-1356 Lobo Álvarez, Beatriz|||0000-0003-3391-7125 Rodiño-Janeiro, Bruno K.|||0000-0002-0633-6774 Pigrau Pastor, Marc|||0000-0001-6801-3017 Sánchez Chardi, Alejandro|||0000-0002-8789-1883 González Castro, Ana Maria|||0000-0003-3955-3318 Fortea, Marina|||0000-0001-8885-7495 Pardo-Camacho, Cristina Nieto Ruiz, Adoración|||0000-0002-3421-2840 Expósito, Elba|||0000-0001-6088-3149 Guagnozzi, Danila|||0000-0002-6171-1901 Rodríguez-Urrutia, Amanda|||0000-0001-6882-205X de Torres, Inés|||0000-0002-5495-9140 Farré, Ricard|||0000-0001-7158-171X Azpiroz Vidaur, Fernando|||0000-0002-7327-960X Alonso Cotoner, Carmen|||0000-0002-3483-2919 Santos, Javier|||0000-0002-4798-5033 Vicario Perez, Maria|||0000-0001-9622-3185 |
| author_role |
author |
| author2 |
Martínez, Cristina|||0000-0002-9368-1356 Lobo Álvarez, Beatriz|||0000-0003-3391-7125 Rodiño-Janeiro, Bruno K.|||0000-0002-0633-6774 Pigrau Pastor, Marc|||0000-0001-6801-3017 Sánchez Chardi, Alejandro|||0000-0002-8789-1883 González Castro, Ana Maria|||0000-0003-3955-3318 Fortea, Marina|||0000-0001-8885-7495 Pardo-Camacho, Cristina Nieto Ruiz, Adoración|||0000-0002-3421-2840 Expósito, Elba|||0000-0001-6088-3149 Guagnozzi, Danila|||0000-0002-6171-1901 Rodríguez-Urrutia, Amanda|||0000-0001-6882-205X de Torres, Inés|||0000-0002-5495-9140 Farré, Ricard|||0000-0001-7158-171X Azpiroz Vidaur, Fernando|||0000-0002-7327-960X Alonso Cotoner, Carmen|||0000-0002-3483-2919 Santos, Javier|||0000-0002-4798-5033 Vicario Perez, Maria|||0000-0001-9622-3185 |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Irritable bowel syndrome Mucosal immunology |
| topic |
Irritable bowel syndrome Mucosal immunology |
| description |
Corticotropin-releasing factor (CRF) has been identified in intestinal mucosal eosinophils and associated with psychological stress and gut dysfunction. Irritable bowel syndrome (IBS) is commonly characterized by altered intestinal motility, immune activation, and increased gut barrier permeability along with heightened susceptibility to psychosocial stress. Despite intensive research, the role of mucosal eosinophils in stress-associated gut dysfunction remains uncertain. In this study, we evaluated eosinophil activation profile and CRF content in the jejunal mucosa of diarrhea-predominant IBS (IBS-D) and healthy controls (HC) by gene/protein expression and transmission electron microscopy. We also explored the association between intestinal eosinophil CRF and chronic stress, and the potential mechanisms underlying the stress response by assessing eosinophil response to neuropeptides. We found that mucosal eosinophils displayed higher degranulation profile in IBS-D as compared to HC, with increased content of CRF in the cytoplasmic granules, which significantly correlated with IBS clinical severity, life stress background and depression. Eosinophils responded to substance P and carbachol by increasing secretory activity and CRF synthesis and release, without promoting pro-inflammatory activity, a profile similar to that found in mucosal eosinophils from IBS-D. Collectively, our results suggest that intestinal mucosal eosinophils are potential contributors to stress-mediated gut dysfunction through CRF production and release. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2 2020-01-01 2020 2020-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/253129 https://dx.doi.org/urn:doi:10.1038/s41598-020-77176-x |
| url |
https://ddd.uab.cat/record/253129 https://dx.doi.org/urn:doi:10.1038/s41598-020-77176-x |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CD15/00010 Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CM10/00155 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI17/01443 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI15/00301 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI19/01643 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CP18/00116 Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CM08/00229 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 FI12/00254 Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 SAF2016-76648-R Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-1285 |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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Universitat Autònoma de Barcelona |
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Dipòsit Digital de Documents de la UAB |
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Dipòsit Digital de Documents de la UAB |
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1869419606635970560 |
| spelling |
Overexpression of corticotropin-releasing factor in intestinal mucosal eosinophils is associated with clinical severity in Diarrhea-Predominant Irritable Bowel SyndromeSalvo-Romero, EloísaMartínez, Cristina|||0000-0002-9368-1356Lobo Álvarez, Beatriz|||0000-0003-3391-7125Rodiño-Janeiro, Bruno K.|||0000-0002-0633-6774Pigrau Pastor, Marc|||0000-0001-6801-3017Sánchez Chardi, Alejandro|||0000-0002-8789-1883González Castro, Ana Maria|||0000-0003-3955-3318Fortea, Marina|||0000-0001-8885-7495Pardo-Camacho, CristinaNieto Ruiz, Adoración|||0000-0002-3421-2840Expósito, Elba|||0000-0001-6088-3149Guagnozzi, Danila|||0000-0002-6171-1901Rodríguez-Urrutia, Amanda|||0000-0001-6882-205Xde Torres, Inés|||0000-0002-5495-9140Farré, Ricard|||0000-0001-7158-171XAzpiroz Vidaur, Fernando|||0000-0002-7327-960XAlonso Cotoner, Carmen|||0000-0002-3483-2919Santos, Javier|||0000-0002-4798-5033Vicario Perez, Maria|||0000-0001-9622-3185Irritable bowel syndromeMucosal immunologyCorticotropin-releasing factor (CRF) has been identified in intestinal mucosal eosinophils and associated with psychological stress and gut dysfunction. Irritable bowel syndrome (IBS) is commonly characterized by altered intestinal motility, immune activation, and increased gut barrier permeability along with heightened susceptibility to psychosocial stress. Despite intensive research, the role of mucosal eosinophils in stress-associated gut dysfunction remains uncertain. In this study, we evaluated eosinophil activation profile and CRF content in the jejunal mucosa of diarrhea-predominant IBS (IBS-D) and healthy controls (HC) by gene/protein expression and transmission electron microscopy. We also explored the association between intestinal eosinophil CRF and chronic stress, and the potential mechanisms underlying the stress response by assessing eosinophil response to neuropeptides. We found that mucosal eosinophils displayed higher degranulation profile in IBS-D as compared to HC, with increased content of CRF in the cytoplasmic granules, which significantly correlated with IBS clinical severity, life stress background and depression. Eosinophils responded to substance P and carbachol by increasing secretory activity and CRF synthesis and release, without promoting pro-inflammatory activity, a profile similar to that found in mucosal eosinophils from IBS-D. Collectively, our results suggest that intestinal mucosal eosinophils are potential contributors to stress-mediated gut dysfunction through CRF production and release. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/253129https://dx.doi.org/urn:doi:10.1038/s41598-020-77176-xreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 CD15/00010Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CM10/00155Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI17/01443Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI15/00301Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI19/01643Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CP18/00116Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CM08/00229Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 FI12/00254Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 SAF2016-76648-RAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-1285open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2531292026-06-06T12:50:31Z |
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15,300724 |