Unique Splicing of Lrp5 in the Brain
Low-density lipoprotein receptor-related protein 5 (LRP5) is a constitutively expressed receptor with observed roles in bone homeostasis, retinal development, and cardiac metabolism. However, the function of LRP5 in the brain remains unexplored. This study investigates LRP5's role in the centra...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:306961 |
| Acceso en línea: | https://ddd.uab.cat/record/306961 https://dx.doi.org/urn:doi:10.3390/ijms25126763 |
| Access Level: | acceso abierto |
| Palabra clave: | LRP5 RNA-seq Brain Liver Retinoic acid Synapse Transcriptome |
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Unique Splicing of Lrp5 in the BrainA New Player in Neurodevelopment and Brain MaturationLuquero, Aureli|||0000-0002-1104-5963Pimentel, NoeliaVilahur, Gemma|||0000-0002-2828-8873Badimon, Lina|||0000-0002-9162-2459Borrell-Pages, Maria|||0000-0002-1759-9756LRP5RNA-seqBrainLiverRetinoic acidSynapseTranscriptomeLow-density lipoprotein receptor-related protein 5 (LRP5) is a constitutively expressed receptor with observed roles in bone homeostasis, retinal development, and cardiac metabolism. However, the function of LRP5 in the brain remains unexplored. This study investigates LRP5's role in the central nervous system by conducting an extensive analysis using RNA-seq tools and in silico assessments. Two protein-coding Lrp5 transcripts are expressed in mice: full-length Lrp5-201 and a truncated form encoded by Lrp5-202. Wt mice express Lrp5-201 in the liver and brain and do not express the truncated form. Lrp5 mice express Lrp5-202 in the liver and brain and do not express Lrp5-201 in the liver. Interestingly, Lrp5 mouse brains show full-length Lrp5-201 expression, suggesting that LRP5 has a role in preserving brain function during development. Functional gene enrichment analysis on RNA-seq unveils dysregulated expression of genes associated with neuronal differentiation and synapse formation in the brains of Lrp5 mice compared to Wt mice. Furthermore, Gene Set Enrichment Analysis highlights downregulated expression of genes involved in retinol and linoleic acid metabolism in Lrp5 mouse brains. Tissue-specific alternative splicing of Lrp5 in Lrp5 mice supports that the expression of LRP5 in the brain is needed for the correct synthesis of vitamins and fatty acids, and it is indispensable for correct brain development.Universitat Autònoma de Barcelona 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/306961https://dx.doi.org/urn:doi:10.3390/ijms25126763reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-107160RB-I00Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PMP22/00108Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CB16/11/00411open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3069612026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Unique Splicing of Lrp5 in the Brain A New Player in Neurodevelopment and Brain Maturation |
| title |
Unique Splicing of Lrp5 in the Brain |
| spellingShingle |
Unique Splicing of Lrp5 in the Brain Luquero, Aureli|||0000-0002-1104-5963 LRP5 RNA-seq Brain Liver Retinoic acid Synapse Transcriptome |
| title_short |
Unique Splicing of Lrp5 in the Brain |
| title_full |
Unique Splicing of Lrp5 in the Brain |
| title_fullStr |
Unique Splicing of Lrp5 in the Brain |
| title_full_unstemmed |
Unique Splicing of Lrp5 in the Brain |
| title_sort |
Unique Splicing of Lrp5 in the Brain |
| dc.creator.none.fl_str_mv |
Luquero, Aureli|||0000-0002-1104-5963 Pimentel, Noelia Vilahur, Gemma|||0000-0002-2828-8873 Badimon, Lina|||0000-0002-9162-2459 Borrell-Pages, Maria|||0000-0002-1759-9756 |
| author |
Luquero, Aureli|||0000-0002-1104-5963 |
| author_facet |
Luquero, Aureli|||0000-0002-1104-5963 Pimentel, Noelia Vilahur, Gemma|||0000-0002-2828-8873 Badimon, Lina|||0000-0002-9162-2459 Borrell-Pages, Maria|||0000-0002-1759-9756 |
| author_role |
author |
| author2 |
Pimentel, Noelia Vilahur, Gemma|||0000-0002-2828-8873 Badimon, Lina|||0000-0002-9162-2459 Borrell-Pages, Maria|||0000-0002-1759-9756 |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Universitat Autònoma de Barcelona |
| dc.subject.none.fl_str_mv |
LRP5 RNA-seq Brain Liver Retinoic acid Synapse Transcriptome |
| topic |
LRP5 RNA-seq Brain Liver Retinoic acid Synapse Transcriptome |
| description |
Low-density lipoprotein receptor-related protein 5 (LRP5) is a constitutively expressed receptor with observed roles in bone homeostasis, retinal development, and cardiac metabolism. However, the function of LRP5 in the brain remains unexplored. This study investigates LRP5's role in the central nervous system by conducting an extensive analysis using RNA-seq tools and in silico assessments. Two protein-coding Lrp5 transcripts are expressed in mice: full-length Lrp5-201 and a truncated form encoded by Lrp5-202. Wt mice express Lrp5-201 in the liver and brain and do not express the truncated form. Lrp5 mice express Lrp5-202 in the liver and brain and do not express Lrp5-201 in the liver. Interestingly, Lrp5 mouse brains show full-length Lrp5-201 expression, suggesting that LRP5 has a role in preserving brain function during development. Functional gene enrichment analysis on RNA-seq unveils dysregulated expression of genes associated with neuronal differentiation and synapse formation in the brains of Lrp5 mice compared to Wt mice. Furthermore, Gene Set Enrichment Analysis highlights downregulated expression of genes involved in retinol and linoleic acid metabolism in Lrp5 mouse brains. Tissue-specific alternative splicing of Lrp5 in Lrp5 mice supports that the expression of LRP5 in the brain is needed for the correct synthesis of vitamins and fatty acids, and it is indispensable for correct brain development. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2 2024-01-01 2024 2024-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/306961 https://dx.doi.org/urn:doi:10.3390/ijms25126763 |
| url |
https://ddd.uab.cat/record/306961 https://dx.doi.org/urn:doi:10.3390/ijms25126763 |
| dc.language.none.fl_str_mv |
Inglés eng |
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Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-107160RB-I00 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PMP22/00108 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CB16/11/00411 |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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Universitat Autònoma de Barcelona |
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