Host-dependent editing of SARS-CoV-2 in COVID-19 patients

A common trait among RNA viruses is their high capability to acquire genetic variability due to viral and host mechanisms. Next-generation sequencing (NGS) analysis enables the deep study of the viral quasispecies in samples from infected individuals. In this study, the viral quasispecies complexity...

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Detalhes bibliográficos
Autores: Gregori i Font, Josep|||0000-0002-4253-8015, Cortese, Maria Francesca|||0000-0002-4318-532X, Piñana, Maria|||0000-0002-4766-2613, Campos, Carolina|||0000-0002-0132-7027, Garcia-Cehic, D.|||0000-0002-0009-038X, Andrés, Cristina|||0000-0002-3200-0895, Abril, Josep Francesc|||0000-0001-7793-589X, Codina, María Gema|||0000-0002-5313-5086, Rando-Segura, Ariadna|||0000-0003-4555-7286, Esperalba, Juliana|||0000-0003-1326-1341, Sulleiro, Elena|||0000-0002-9783-6060, Joseph-Munné, Joan, Saubi, Narcis|||0000-0001-5493-1044, Colomer-Castell, Sergi|||0000-0002-0307-7455, Martin, Mari Carmen|||0000-0003-1327-8813, Castillo, Carla, Esteban Mur, Juan Ignacio|||0000-0001-5085-917X, Pumarola Suñé, Tomàs|||0000-0002-5171-7461, Rodríguez Frías, Francisco|||0000-0002-9128-7013, Antón Pagarolas, Andrés, 1976-|||0000-0002-1476-0815, Quer, Josep|||0000-0003-0014-084X
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:dnet:uabarcelona_::34cf18eff9b6b94fd8233361340efbd0
Acesso em linha:https://ddd.uab.cat/record/327703
https://dx.doi.org/urn:doi:10.1080/22221751.2021.1969868
Access Level:acceso abierto
Palavra-chave:ADAR1
SARS-CoV-2
Editing
Mutations
Quasispecies
Descrição
Resumo:A common trait among RNA viruses is their high capability to acquire genetic variability due to viral and host mechanisms. Next-generation sequencing (NGS) analysis enables the deep study of the viral quasispecies in samples from infected individuals. In this study, the viral quasispecies complexity and single nucleotide polymorphisms of the SARS-CoV-2 spike gene of coronavirus disease 2019 (COVID-19) patients with mild or severe disease were investigated using next-generation sequencing (Illumina platform). SARS-CoV-2 spike variability was higher in patients with long-lasting infection. Most substitutions found were present at frequencies lower than 1%, and had an A → G or T → C pattern, consistent with variants caused by adenosine deaminase acting on RNA-1 (ADAR1). ADAR1 affected a small fraction of replicating genomes, but produced multiple, mainly non-synonymous mutations. ADAR1 editing during replication rather than the RNA-dependent RNA polymerase (nsp12) was the predominant mechanism generating SARS-CoV-2 genetic variability. However, the mutations produced are not fixed in the infected human population, suggesting that ADAR1 may have an antiviral role, whereas nsp12-induced mutations occurring in patients with high viremia and persistent infection are the main source of new SARS-CoV-2 variants.

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