Transcriptional heterogeneity of IgM+ cells in rainbow trout (Oncorhynchus mykiss) tissues

Two major classes of B lymphocytes have been described to date in rainbow trout IgM+ and IgT+ cells. IgM+ cells are mainly localized in the spleen, peripheral blood and kidney but are also found in other tissues. However, differences among IgM+ cell populations attending to its location are poorly d...

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Autores: Abós, Beatriz, Castro, Rosario, Pignatelli Garrigos, Jaime, Luque, Alfonso, González, Lucía, Tafalla, Carolina
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/293193
Acceso en línea:http://hdl.handle.net/10261/293193
Access Level:acceso abierto
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spelling Transcriptional heterogeneity of IgM+ cells in rainbow trout (Oncorhynchus mykiss) tissuesAbós, BeatrizCastro, RosarioPignatelli Garrigos, JaimeLuque, AlfonsoGonzález, LucíaTafalla, CarolinaTwo major classes of B lymphocytes have been described to date in rainbow trout IgM+ and IgT+ cells. IgM+ cells are mainly localized in the spleen, peripheral blood and kidney but are also found in other tissues. However, differences among IgM+ cell populations attending to its location are poorly defined in fish. Thus, the aim of this work was to characterize the expression of different immune molecules such as chemokine receptors, Toll-like receptors (TLRs) and transcription factors on sorted IgM+ lymphocytes from different rainbow trout tissues. IgM+ populations from blood, spleen, kidney, gills, intestine and liver were isolated by cell sorting and the constitutive levels of transcription of these genes evaluated by real-time PCR. To further characterize B cells, we identified an MS4A sequence. In humans, the MS4A family includes several genes with immune functions, such as the B cell marker CD20 or FcRβ. Subsequently, we have also evaluated the mRNA levels of this MS4A gene in the different IgM + populations. The relevant differences in transcriptional patterns observed for each of these IgM+ populations analyzed, point to the presence of functionally different tissue-specific B cell populations in rainbow trout. The data shown provides a pattern of genes transcribed in IgM + B cells not previously revealed in teleost fish. Furthermore, the constitutive expression of all the TLR genes analyzed in IgM+ cells suggests an important role for these cells in innate immunity. © 2013 Abós et al.Peer reviewedPublic Library of ScienceAbós, Beatriz [0000-0002-3402-7397]Tafalla, Carolina [0000-0002-0860-2976]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/293193reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro de Investigación en Sanidad Animal (CISA)Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2931932026-05-22T06:33:51Z
dc.title.none.fl_str_mv Transcriptional heterogeneity of IgM+ cells in rainbow trout (Oncorhynchus mykiss) tissues
title Transcriptional heterogeneity of IgM+ cells in rainbow trout (Oncorhynchus mykiss) tissues
spellingShingle Transcriptional heterogeneity of IgM+ cells in rainbow trout (Oncorhynchus mykiss) tissues
Abós, Beatriz
title_short Transcriptional heterogeneity of IgM+ cells in rainbow trout (Oncorhynchus mykiss) tissues
title_full Transcriptional heterogeneity of IgM+ cells in rainbow trout (Oncorhynchus mykiss) tissues
title_fullStr Transcriptional heterogeneity of IgM+ cells in rainbow trout (Oncorhynchus mykiss) tissues
title_full_unstemmed Transcriptional heterogeneity of IgM+ cells in rainbow trout (Oncorhynchus mykiss) tissues
title_sort Transcriptional heterogeneity of IgM+ cells in rainbow trout (Oncorhynchus mykiss) tissues
dc.creator.none.fl_str_mv Abós, Beatriz
Castro, Rosario
Pignatelli Garrigos, Jaime
Luque, Alfonso
González, Lucía
Tafalla, Carolina
author Abós, Beatriz
author_facet Abós, Beatriz
Castro, Rosario
Pignatelli Garrigos, Jaime
Luque, Alfonso
González, Lucía
Tafalla, Carolina
author_role author
author2 Castro, Rosario
Pignatelli Garrigos, Jaime
Luque, Alfonso
González, Lucía
Tafalla, Carolina
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Abós, Beatriz [0000-0002-3402-7397]
Tafalla, Carolina [0000-0002-0860-2976]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Two major classes of B lymphocytes have been described to date in rainbow trout IgM+ and IgT+ cells. IgM+ cells are mainly localized in the spleen, peripheral blood and kidney but are also found in other tissues. However, differences among IgM+ cell populations attending to its location are poorly defined in fish. Thus, the aim of this work was to characterize the expression of different immune molecules such as chemokine receptors, Toll-like receptors (TLRs) and transcription factors on sorted IgM+ lymphocytes from different rainbow trout tissues. IgM+ populations from blood, spleen, kidney, gills, intestine and liver were isolated by cell sorting and the constitutive levels of transcription of these genes evaluated by real-time PCR. To further characterize B cells, we identified an MS4A sequence. In humans, the MS4A family includes several genes with immune functions, such as the B cell marker CD20 or FcRβ. Subsequently, we have also evaluated the mRNA levels of this MS4A gene in the different IgM + populations. The relevant differences in transcriptional patterns observed for each of these IgM+ populations analyzed, point to the presence of functionally different tissue-specific B cell populations in rainbow trout. The data shown provides a pattern of genes transcribed in IgM + B cells not previously revealed in teleost fish. Furthermore, the constitutive expression of all the TLR genes analyzed in IgM+ cells suggests an important role for these cells in innate immunity. © 2013 Abós et al.
publishDate 2013
dc.date.none.fl_str_mv 2013
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/293193
url http://hdl.handle.net/10261/293193
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro de Investigación en Sanidad Animal (CISA)
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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