The strong seasonality of soil microbial community structure in declining Mediterranean pine forests depends more on soil conditions than on tree vitality

The soil microbiome plays an important role in forest functioning. However, the impact of drought-induced dieback and tree death on soil microbial biomass, community structure, and functional composition is unknown. We also lack understanding on how soil microbiota varies seasonally in such declinin...

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Autores: Gazol Burgos, Antonio, González de Andrés, Ester, Valverde Portal, Ángel, Igual, José Mariano, Serrano, Abel, Camarero, Jesús Julio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/378921
Acceso en línea:http://hdl.handle.net/10261/378921
https://api.elsevier.com/content/abstract/scopus_id/85210054717
Access Level:acceso abierto
Palabra clave:Bacteria
Soil microbial biomass
Fungi
Aleppo pine
Maritime pine
PLFA
Scots pine
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repository_id_str
dc.title.none.fl_str_mv The strong seasonality of soil microbial community structure in declining Mediterranean pine forests depends more on soil conditions than on tree vitality
title The strong seasonality of soil microbial community structure in declining Mediterranean pine forests depends more on soil conditions than on tree vitality
spellingShingle The strong seasonality of soil microbial community structure in declining Mediterranean pine forests depends more on soil conditions than on tree vitality
Gazol Burgos, Antonio
Bacteria
Soil microbial biomass
Fungi
Aleppo pine
Maritime pine
PLFA
Scots pine
title_short The strong seasonality of soil microbial community structure in declining Mediterranean pine forests depends more on soil conditions than on tree vitality
title_full The strong seasonality of soil microbial community structure in declining Mediterranean pine forests depends more on soil conditions than on tree vitality
title_fullStr The strong seasonality of soil microbial community structure in declining Mediterranean pine forests depends more on soil conditions than on tree vitality
title_full_unstemmed The strong seasonality of soil microbial community structure in declining Mediterranean pine forests depends more on soil conditions than on tree vitality
title_sort The strong seasonality of soil microbial community structure in declining Mediterranean pine forests depends more on soil conditions than on tree vitality
dc.creator.none.fl_str_mv Gazol Burgos, Antonio
González de Andrés, Ester
Valverde Portal, Ángel
Igual, José Mariano
Serrano, Abel
Camarero, Jesús Julio
author Gazol Burgos, Antonio
author_facet Gazol Burgos, Antonio
González de Andrés, Ester
Valverde Portal, Ángel
Igual, José Mariano
Serrano, Abel
Camarero, Jesús Julio
author_role author
author2 González de Andrés, Ester
Valverde Portal, Ángel
Igual, José Mariano
Serrano, Abel
Camarero, Jesús Julio
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas (España)
Junta de Castilla y León
European Commission
Gazol Burgos, Antonio [0000-0001-5902-9543]
Valverde Portal, Ángel [0000-0003-0439-9605]
Igual, José Mariano [0000-0002-5080-0378]
Camarero, Jesús Julio [0000-0003-2436-2922]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacteria
Soil microbial biomass
Fungi
Aleppo pine
Maritime pine
PLFA
Scots pine
topic Bacteria
Soil microbial biomass
Fungi
Aleppo pine
Maritime pine
PLFA
Scots pine
description The soil microbiome plays an important role in forest functioning. However, the impact of drought-induced dieback and tree death on soil microbial biomass, community structure, and functional composition is unknown. We also lack understanding on how soil microbiota varies seasonally in such declining stands. We used Phospholipid Fatty Acids (PLFA) analysis to quantify soil microbial biomass and study its seasonal changes in three Mediterranean forests showing dieback and dominated by three pine species (Pinus halepensis Mill, Pinus pinaster Ait. and Pinus sylvestris L.). We also measured microclimatic parameters and soil physical and chemical parameters under trees with different vigor, assessed as canopy defoliation degree, and related them to seasonal changes in the soil microbial community. We found marked differences in soil microbial community structure, total biomass, and the relative abundance of major functional groups among forests. First, soil microbial biomass peaked either in the dry summer (P. halepensis) or in autumn (P. pinaster and P. sylvestris). Accordingly, the relative abundance of most functional groups, excluding Arbuscular mycorrhizal fungi, displayed substantial variation between seasons. In addition, the relative abundance of fungi and Gram-positive bacteria exhibited an opposite pattern compared to actinomycetes and Gram-negative bacteria. Second, soil physical and chemical parameters had a significant impact on within-site PLFA variation, although their influence was less important than that of seasonal variation. Third, differences between defoliated and healthy trees were minor and restricted to averaged ratios between different PLFA markers. Overall, the structure, biomass and relative abundance of major functional groups of the soil microbiome vary considerably among stand types and seasons in forests showing ongoing dieback and high mortality. However, while the seasonal dynamics show predictable patterns, which should be accounted for in future studies, the within-site variation is highly variable and mainly depends on soil physical and chemical parameters.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/378921
https://api.elsevier.com/content/abstract/scopus_id/85210054717
url http://hdl.handle.net/10261/378921
https://api.elsevier.com/content/abstract/scopus_id/85210054717
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-123675OB-C43
info:eu-repo/grantAgreement/AEI//TED2021-129770B-C21
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.scitotenv.2024.177560
https://doi.org/10.1016/j.scitotenv.2024.177560

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dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
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instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
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spelling The strong seasonality of soil microbial community structure in declining Mediterranean pine forests depends more on soil conditions than on tree vitalityGazol Burgos, AntonioGonzález de Andrés, EsterValverde Portal, ÁngelIgual, José MarianoSerrano, AbelCamarero, Jesús JulioBacteriaSoil microbial biomassFungiAleppo pineMaritime pinePLFAScots pineThe soil microbiome plays an important role in forest functioning. However, the impact of drought-induced dieback and tree death on soil microbial biomass, community structure, and functional composition is unknown. We also lack understanding on how soil microbiota varies seasonally in such declining stands. We used Phospholipid Fatty Acids (PLFA) analysis to quantify soil microbial biomass and study its seasonal changes in three Mediterranean forests showing dieback and dominated by three pine species (Pinus halepensis Mill, Pinus pinaster Ait. and Pinus sylvestris L.). We also measured microclimatic parameters and soil physical and chemical parameters under trees with different vigor, assessed as canopy defoliation degree, and related them to seasonal changes in the soil microbial community. We found marked differences in soil microbial community structure, total biomass, and the relative abundance of major functional groups among forests. First, soil microbial biomass peaked either in the dry summer (P. halepensis) or in autumn (P. pinaster and P. sylvestris). Accordingly, the relative abundance of most functional groups, excluding Arbuscular mycorrhizal fungi, displayed substantial variation between seasons. In addition, the relative abundance of fungi and Gram-positive bacteria exhibited an opposite pattern compared to actinomycetes and Gram-negative bacteria. Second, soil physical and chemical parameters had a significant impact on within-site PLFA variation, although their influence was less important than that of seasonal variation. Third, differences between defoliated and healthy trees were minor and restricted to averaged ratios between different PLFA markers. Overall, the structure, biomass and relative abundance of major functional groups of the soil microbiome vary considerably among stand types and seasons in forests showing ongoing dieback and high mortality. However, while the seasonal dynamics show predictable patterns, which should be accounted for in future studies, the within-site variation is highly variable and mainly depends on soil physical and chemical parameters.During the course of this experiment, AG was supported by the “Ramón y Cajal” Program of the Spanish MICINN under Grant RyC2020-030647-I, and by CSIC under grant PIE-20223AT003. This research was funded by the Spanish Science and Innovation Ministry & AEI (projects PID2021-123675OB-C43 and TED2021-129770B-C21). AV and JMI were supported by project “CLU-2019-05—IRNASA/CSIC Unit of Excellence”, funded by the Junta de Castilla y León and co-financed by the European Union (ERDF “Europe drives our growth”).Peer reviewedElsevier BVMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas (España)Junta de Castilla y LeónEuropean CommissionGazol Burgos, Antonio [0000-0001-5902-9543]Valverde Portal, Ángel [0000-0003-0439-9605]Igual, José Mariano [0000-0002-5080-0378]Camarero, Jesús Julio [0000-0003-2436-2922]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/378921https://api.elsevier.com/content/abstract/scopus_id/85210054717reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//RyC2020-030647-Iinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-123675OB-C43info:eu-repo/grantAgreement/AEI//TED2021-129770B-C21The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.scitotenv.2024.177560https://doi.org/10.1016/j.scitotenv.2024.177560Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3789212026-05-22T06:33:51Z
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