Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant

17 p.-7 fig.

Detalhes bibliográficos
Autores: Cañadas, O., García-García, Andrea, Prieto, María Auxiliadora, Pérez-Gil, Jesús
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/260036
Acesso em linha:http://hdl.handle.net/10261/260036
Access Level:Acceso aberto
Palavra-chave:DPPC
POPG
PHA
Dynamic light scattering
Lipid monolayers
Relaxation kinetics
Epifluorescence microscopy
Polyhydroxyalkanoates
PHA nanoparticles
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spelling Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactantCañadas, O.García-García, AndreaPrieto, María AuxiliadoraPérez-Gil, JesúsDPPCPOPGPHADynamic light scatteringLipid monolayersRelaxation kineticsEpifluorescence microscopyPolyhydroxyalkanoatesPHA nanoparticles17 p.-7 fig.Polyhydroxyalkanoates (PHA) are polyesters produced intracellularly by many bacterial species as energy storage materials, which are used in biomedical applications, including drug delivery systems, due to their biocompatibility and biodegradability. In this study, we evaluated the potential application of this nanomaterial as a basis of inhaled drug delivery systems. To that end, we assessed the possible interaction between PHA nanoparticles (NPs) and pulmonary surfactant using dynamic light scattering, Langmuir balances, and epifluorescence microscopy. Our results demonstrate that NPs deposited onto preformed monolayers of DPPC or DPPC/POPG bind these surfactant lipids. This interaction facilitated the translocation of the nanomaterial towards the aqueous subphase, with the subsequent loss of lipid from the interface. NPs that remained at the interface associated with liquid expanded (LE)/tilted condensed (TC) phase boundaries, decreasing the size of condensed domains and promoting the intermixing of TC and LE phases at submicroscopic scale. This provided the stability necessary for attaining high surface pressures upon compression, countering the destabilization induced by lipid loss. These effects were observed only for high NP loads, suggesting a limit for the use of these NPs in pulmonary drug delivery.Research in the laboratory of the authors is currently funded by grants from the Spanish Ministry of Science, Universities and Innovation (RTI2018-094564-B-I00, BIO2017-83448-R) and the Regional Government of Madrid (P2018/NMT-4389).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Comunidad de MadridCañadas, O. [0000-0002-1932-3796]Prieto, María Auxiliadora [0000-0002-8038-1223]Pérez-Gil, Jesús [0000-0003-3587-7147]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/260036reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094564-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83448-Rhttps://doi.org/10.3390/nano11061482Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2600362026-05-22T06:33:51Z
dc.title.none.fl_str_mv Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant
title Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant
spellingShingle Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant
Cañadas, O.
DPPC
POPG
PHA
Dynamic light scattering
Lipid monolayers
Relaxation kinetics
Epifluorescence microscopy
Polyhydroxyalkanoates
PHA nanoparticles
title_short Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant
title_full Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant
title_fullStr Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant
title_full_unstemmed Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant
title_sort Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant
dc.creator.none.fl_str_mv Cañadas, O.
García-García, Andrea
Prieto, María Auxiliadora
Pérez-Gil, Jesús
author Cañadas, O.
author_facet Cañadas, O.
García-García, Andrea
Prieto, María Auxiliadora
Pérez-Gil, Jesús
author_role author
author2 García-García, Andrea
Prieto, María Auxiliadora
Pérez-Gil, Jesús
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Comunidad de Madrid
Cañadas, O. [0000-0002-1932-3796]
Prieto, María Auxiliadora [0000-0002-8038-1223]
Pérez-Gil, Jesús [0000-0003-3587-7147]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv DPPC
POPG
PHA
Dynamic light scattering
Lipid monolayers
Relaxation kinetics
Epifluorescence microscopy
Polyhydroxyalkanoates
PHA nanoparticles
topic DPPC
POPG
PHA
Dynamic light scattering
Lipid monolayers
Relaxation kinetics
Epifluorescence microscopy
Polyhydroxyalkanoates
PHA nanoparticles
description 17 p.-7 fig.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/260036
url http://hdl.handle.net/10261/260036
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094564-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83448-R
https://doi.org/10.3390/nano11061482
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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