Differential expression of Ran GTPase during HMBA-induced differentiation in murine erythroleukemia cells

9.-5 fig.

Detalles Bibliográficos
Autores: Vanegas, N., García-Sacristán, Ana, López-Fernández, Luis A., Párraga, Mario, Del Mazo, Jesús, Hernandez, Pablo, Schvartzman, Jorge Bernardo, Krimer, Dora B.
Tipo de recurso: artículo
Fecha de publicación:2003
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::2e8a80c275222dd1d9d73623a4afccb4
Acceso en línea:http://hdl.handle.net/10261/209001
Access Level:acceso abierto
Palabra clave:Erythroleukemia cells
Hexamethylene bisacetamide (HMBA)
Ran GTPase
Differential gene expression
id ES_cb34fd1aa10afd282fd4feb0c2d688bd
oai_identifier_str oai:dnet:digitalcsic_::2e8a80c275222dd1d9d73623a4afccb4
network_acronym_str ES
network_name_str España
repository_id_str
spelling Differential expression of Ran GTPase during HMBA-induced differentiation in murine erythroleukemia cellsVanegas, N.García-Sacristán, AnaLópez-Fernández, Luis A.Párraga, MarioDel Mazo, JesúsHernandez, PabloSchvartzman, Jorge BernardoKrimer, Dora B.Erythroleukemia cellsHexamethylene bisacetamide (HMBA)Ran GTPaseDifferential gene expression9.-5 fig.Murine erythroleukemia (MEL) cells undergo erythroid differentiation in vitro when treated with hexamethylene bisacetamide (HMBA).To identify genes involved in the commitment of MEL cells to differentiate, we screened a cDNA library constructed from HMBA-inducedcells by differential hybridization and isolated GTPaseRanas a down-regulated gene. We observed thatRanwas expressed in a biphasicmode. Following a decrease in mRNA level during the initial hours of induction,Ranre-expressed at 24–48 h, and gradually declinedagain. To investigate the role ofRanduring MEL differentiation we constructed MEL transfectants capable to express or blockRanmRNAproduction constitutively. No effects were observed on cell growth and proliferation. Blockage of Ran, however, interfered with MEL celldifferentiation resulting in a decrease of cell survival in the committed population.Thiswork was supported by grant PM97-0138 (Spanish Ministry of Education), grant 08.1/0067.1/2001.1 (CAM, Regional Government of Madrid) and grant 99/0850 (FIS, Spanish Ministry of Health). JM was supported by grants from DGI-CYT (PB95-0119), EU (PL-960183) and CAM (07B/0022).Peer reviewedElsevierMinisterio de Educación (España)Comunidad de MadridMinisterio de Sanidad (España)European CommissionGarcía-Sacristán, Ana [0000-0002-3587-3560]López-Fernández, Luis A. [0000-0003-3964-5837]Párraga, Mario [0000-0002-3365-3070]Del Mazo, Jesús [0000-0003-3269-3895]Hernandez, Pablo [0000-0002-8113-1277]Schvartzman, Jorge Bernardo [0000-0002-1856-6278]Krimer, Dora B. [0000-0001-9972-3994]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202003info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/209001reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/s0145-2126(02)00231-xSíinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::2e8a80c275222dd1d9d73623a4afccb42026-05-22T06:33:51Z
dc.title.none.fl_str_mv Differential expression of Ran GTPase during HMBA-induced differentiation in murine erythroleukemia cells
title Differential expression of Ran GTPase during HMBA-induced differentiation in murine erythroleukemia cells
spellingShingle Differential expression of Ran GTPase during HMBA-induced differentiation in murine erythroleukemia cells
Vanegas, N.
Erythroleukemia cells
Hexamethylene bisacetamide (HMBA)
Ran GTPase
Differential gene expression
title_short Differential expression of Ran GTPase during HMBA-induced differentiation in murine erythroleukemia cells
title_full Differential expression of Ran GTPase during HMBA-induced differentiation in murine erythroleukemia cells
title_fullStr Differential expression of Ran GTPase during HMBA-induced differentiation in murine erythroleukemia cells
title_full_unstemmed Differential expression of Ran GTPase during HMBA-induced differentiation in murine erythroleukemia cells
title_sort Differential expression of Ran GTPase during HMBA-induced differentiation in murine erythroleukemia cells
dc.creator.none.fl_str_mv Vanegas, N.
García-Sacristán, Ana
López-Fernández, Luis A.
Párraga, Mario
Del Mazo, Jesús
Hernandez, Pablo
Schvartzman, Jorge Bernardo
Krimer, Dora B.
author Vanegas, N.
author_facet Vanegas, N.
García-Sacristán, Ana
López-Fernández, Luis A.
Párraga, Mario
Del Mazo, Jesús
Hernandez, Pablo
Schvartzman, Jorge Bernardo
Krimer, Dora B.
author_role author
author2 García-Sacristán, Ana
López-Fernández, Luis A.
Párraga, Mario
Del Mazo, Jesús
Hernandez, Pablo
Schvartzman, Jorge Bernardo
Krimer, Dora B.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación (España)
Comunidad de Madrid
Ministerio de Sanidad (España)
European Commission
García-Sacristán, Ana [0000-0002-3587-3560]
López-Fernández, Luis A. [0000-0003-3964-5837]
Párraga, Mario [0000-0002-3365-3070]
Del Mazo, Jesús [0000-0003-3269-3895]
Hernandez, Pablo [0000-0002-8113-1277]
Schvartzman, Jorge Bernardo [0000-0002-1856-6278]
Krimer, Dora B. [0000-0001-9972-3994]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Erythroleukemia cells
Hexamethylene bisacetamide (HMBA)
Ran GTPase
Differential gene expression
topic Erythroleukemia cells
Hexamethylene bisacetamide (HMBA)
Ran GTPase
Differential gene expression
description 9.-5 fig.
publishDate 2003
dc.date.none.fl_str_mv 2003
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/209001
url http://hdl.handle.net/10261/209001
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/s0145-2126(02)00231-x
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419567807201280
score 15,81155

Similares en LA Referencia