Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature

Hoyo Pérez, Javier|||0000-0002-9927-2465; Torrent Burgués, Juan|||0000-0002-4952-736X; Tzanov, Tzanko|||0000-0002-8568-1110

Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature

The current strategies to eradicate bacteria require that the antimicrobial agent either penetrate or disrupt the bacterial membrane. In Escherichia coli (E.coli) as a model of Gram-negative strains, the antimicrobials have to cross two barriers – the outer and the inner membrane being the latter co...

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Autores:	Hoyo Pérez, Javier\|\|\|0000-0002-9927-2465, Torrent Burgués, Juan\|\|\|0000-0002-4952-736X, Tzanov, Tzanko\|\|\|0000-0002-8568-1110
Tipo de recurso:	artículo
Fecha de publicación:	2020
País:	España
Institución:	Universitat Politècnica de Catalunya (UPC)
Repositorio:	UPCommons. Portal del coneixement obert de la UPC
Idioma:	inglés
OAI Identifier:	oai:upcommons.upc.edu:2117/183514
Acceso en línea:	https://hdl.handle.net/2117/183514 https://dx.doi.org/10.4149/gpb_2019063
Access Level:	acceso abierto
Palabra clave:	Lipids Anti-infective agents Escheríchia coli, Infeccions per Cell membranes Phosphatidylethanolamine Phosphatidylglycerol Cardiolipin Lipid-lipid interactions Bacterial mimetic membranes Physiological temperature Lípids Agents antiinfecciosos Infeccions per escheríchia coli Membranes cel·lulars Àrees temàtiques de la UPC::Enginyeria química

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spelling	Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperatureHoyo Pérez, Javier\|\|\|0000-0002-9927-2465Torrent Burgués, Juan\|\|\|0000-0002-4952-736XTzanov, Tzanko\|\|\|0000-0002-8568-1110LipidsAnti-infective agentsEscheríchia coli, Infeccions perCell membranesPhosphatidylethanolaminePhosphatidylglycerolCardiolipinLipid-lipid interactionsBacterial mimetic membranesPhysiological temperatureLípidsAgents antiinfecciososInfeccions per escheríchia coliMembranes cel·lularsÀrees temàtiques de la UPC::Enginyeria químicaThe current strategies to eradicate bacteria require that the antimicrobial agent either penetrate or disrupt the bacterial membrane. In Escherichia coli (E.coli) as a model of Gram-negative strains, the antimicrobials have to cross two barriers – the outer and the inner membrane being the latter composed by ~ 77% phosphatidylethanolamine (PE), ~ 13% phosphatidylglycerol (PG) and ~ 10% cardiolipin (CL) lipids. Each one of these lipid families shares the same headgroup, but contains acyl chains with varying length and degree of unsaturation. Bacteria adapt their membrane lipid composition and metabolism in response to environmental signals, such as the temperature, resulting in different interactions with exogenous molecules, e.g. antibacterial agents. Herein, bacterial model membranes are prepared to evaluate the lipid-lipid interactions in Langmuir monolayers of binary mixtures at several molar ratios of PE and PG or CL at human physiological temperature (37°C). Both PE:PG and PE:CL monolayers were stable at 37°C and presented higher molecular areas (> 20 Å2/molecule) than at 23°C. However, these lipid mixtures presented liquid-expanded state and rigidity (inverse of the compressibility modulus ~ 90 mN/m) slightly lower than at 23°C. Such athermalicity at biologically relevant temperatures may favour the preservation of the biological functions of E.coliPeer Reviewed20202020-03-2620202020-04-16journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/183514https://dx.doi.org/10.4149/gpb_2019063reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)InglésengEuropean Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 720851 Pre-commercial lines for production of surface nanostructured antimicrobial and anti-biofilm textiles, medical devices and water treatment membranesopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1835142026-05-27T15:37:01Z
dc.title.none.fl_str_mv	Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature
title	Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature
spellingShingle	Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature Hoyo Pérez, Javier\|\|\|0000-0002-9927-2465 Lipids Anti-infective agents Escheríchia coli, Infeccions per Cell membranes Phosphatidylethanolamine Phosphatidylglycerol Cardiolipin Lipid-lipid interactions Bacterial mimetic membranes Physiological temperature Lípids Agents antiinfecciosos Infeccions per escheríchia coli Membranes cel·lulars Àrees temàtiques de la UPC::Enginyeria química
title_short	Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature
title_full	Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature
title_fullStr	Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature
title_full_unstemmed	Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature
title_sort	Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature
dc.creator.none.fl_str_mv	Hoyo Pérez, Javier\|\|\|0000-0002-9927-2465 Torrent Burgués, Juan\|\|\|0000-0002-4952-736X Tzanov, Tzanko\|\|\|0000-0002-8568-1110
author	Hoyo Pérez, Javier\|\|\|0000-0002-9927-2465
author_facet	Hoyo Pérez, Javier\|\|\|0000-0002-9927-2465 Torrent Burgués, Juan\|\|\|0000-0002-4952-736X Tzanov, Tzanko\|\|\|0000-0002-8568-1110
author_role	author
author2	Torrent Burgués, Juan\|\|\|0000-0002-4952-736X Tzanov, Tzanko\|\|\|0000-0002-8568-1110
author2_role	author author
dc.subject.none.fl_str_mv	Lipids Anti-infective agents Escheríchia coli, Infeccions per Cell membranes Phosphatidylethanolamine Phosphatidylglycerol Cardiolipin Lipid-lipid interactions Bacterial mimetic membranes Physiological temperature Lípids Agents antiinfecciosos Infeccions per escheríchia coli Membranes cel·lulars Àrees temàtiques de la UPC::Enginyeria química
topic	Lipids Anti-infective agents Escheríchia coli, Infeccions per Cell membranes Phosphatidylethanolamine Phosphatidylglycerol Cardiolipin Lipid-lipid interactions Bacterial mimetic membranes Physiological temperature Lípids Agents antiinfecciosos Infeccions per escheríchia coli Membranes cel·lulars Àrees temàtiques de la UPC::Enginyeria química
description	The current strategies to eradicate bacteria require that the antimicrobial agent either penetrate or disrupt the bacterial membrane. In Escherichia coli (E.coli) as a model of Gram-negative strains, the antimicrobials have to cross two barriers – the outer and the inner membrane being the latter composed by ~ 77% phosphatidylethanolamine (PE), ~ 13% phosphatidylglycerol (PG) and ~ 10% cardiolipin (CL) lipids. Each one of these lipid families shares the same headgroup, but contains acyl chains with varying length and degree of unsaturation. Bacteria adapt their membrane lipid composition and metabolism in response to environmental signals, such as the temperature, resulting in different interactions with exogenous molecules, e.g. antibacterial agents. Herein, bacterial model membranes are prepared to evaluate the lipid-lipid interactions in Langmuir monolayers of binary mixtures at several molar ratios of PE and PG or CL at human physiological temperature (37°C). Both PE:PG and PE:CL monolayers were stable at 37°C and presented higher molecular areas (> 20 Å2/molecule) than at 23°C. However, these lipid mixtures presented liquid-expanded state and rigidity (inverse of the compressibility modulus ~ 90 mN/m) slightly lower than at 23°C. Such athermalicity at biologically relevant temperatures may favour the preservation of the biological functions of E.coli
publishDate	2020
dc.date.none.fl_str_mv	2020 2020-03-26 2020 2020-04-16
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2117/183514 https://dx.doi.org/10.4149/gpb_2019063
url	https://hdl.handle.net/2117/183514 https://dx.doi.org/10.4149/gpb_2019063
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	European Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 720851 Pre-commercial lines for production of surface nanostructured antimicrobial and anti-biofilm textiles, medical devices and water treatment membranes
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:UPCommons. Portal del coneixement obert de la UPC instname:Universitat Politècnica de Catalunya (UPC)
instname_str	Universitat Politècnica de Catalunya (UPC)
reponame_str	UPCommons. Portal del coneixement obert de la UPC
collection	UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.300724

Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature

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