cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes

[EN] To date, the underlying mechanisms by which cAMP modulators act during in vitro maturation to improve oocyte developmental competence are poorly understood. Here, we sought to fill this knowledge gap by evaluating the use of phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and ade...

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Autores: Medina Chávez, Daniela Alejandra, Sánchez Ajofrín, Irene, Peris Frau, Patricia, Maside Mielgo, Carolina, Montoro, Vidal, Fernández Santos, Rocío, Garde, José Julián, Soler, Ana Josefa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/23608
Acceso en línea:https://www.mdpi.com/2076-2615/11/9/2512
https://hdl.handle.net/10612/23608
Access Level:acceso abierto
Palabra clave:Veterinaria
Sheep
Embryo
Oocyte
In vitro maturation
DNA damage
cAMP
IBMX
Forskolin
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oai_identifier_str oai:buleria.unileon.es:10612/23608
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repository_id_str
spelling cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep OocytesMedina Chávez, Daniela AlejandraSánchez Ajofrín, IrenePeris Frau, PatriciaMaside Mielgo, CarolinaMontoro, VidalFernández Santos, RocíoGarde, José JuliánSoler, Ana JosefaVeterinariaSheepEmbryoOocyteIn vitro maturationDNA damagecAMPIBMXForskolin[EN] To date, the underlying mechanisms by which cAMP modulators act during in vitro maturation to improve oocyte developmental competence are poorly understood. Here, we sought to fill this knowledge gap by evaluating the use of phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and adenylyl cyclase activator forskolin during a culture period of 2 h before in vitro maturation (pre-IVM) on the nuclear and cytoplasmic maturation features in essential organelles, cumulus cells activity, and in vitro developmental potential of sheep oocytes. Results showed that pre-IVM treatment significantly decreased (p < 0.05) the DNA damage of mature oocytes (pre-IVM = 2.08% ± 3.51% vs. control = 20.58% ± 3.51%) and increased (p ≤ 0.05) expanded blastocyst rates compared to the control (from the total of oocytes: pre-IVM = 23.89% ± 1.47% vs. control = 18.22% ± 1.47%, and from the cleaved embryos: pre-IVM = 45.16% ± 1.73% vs. control = 32.88% ± 1.73%). Considering that oocytes are highly vulnerable to the accumulation of DNA damage because of exposure to in vitro culture conditions, our results suggest that the modulation of intra-oocyte cAMP levels with forskolin and IBMX before IVM might afford oocytes a more effective DNA repair mechanism to overcome damage obstacles and ultimately improve developmental competence. This previously unappreciated action of cAMP modulators could help to develop improved methods for assisted reproduction technologies in animal and clinical research.SISpanish Ministry of Economy and CompetitivenessUniversity of Castilla-La Mancha scholarshipsMDPISanidad AnimalFacultad de Veterinaria2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.mdpi.com/2076-2615/11/9/2512https://hdl.handle.net/10612/23608reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónIngléshttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/236082026-06-24T12:43:27Z
dc.title.none.fl_str_mv cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes
title cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes
spellingShingle cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes
Medina Chávez, Daniela Alejandra
Veterinaria
Sheep
Embryo
Oocyte
In vitro maturation
DNA damage
cAMP
IBMX
Forskolin
title_short cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes
title_full cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes
title_fullStr cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes
title_full_unstemmed cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes
title_sort cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes
dc.creator.none.fl_str_mv Medina Chávez, Daniela Alejandra
Sánchez Ajofrín, Irene
Peris Frau, Patricia
Maside Mielgo, Carolina
Montoro, Vidal
Fernández Santos, Rocío
Garde, José Julián
Soler, Ana Josefa
author Medina Chávez, Daniela Alejandra
author_facet Medina Chávez, Daniela Alejandra
Sánchez Ajofrín, Irene
Peris Frau, Patricia
Maside Mielgo, Carolina
Montoro, Vidal
Fernández Santos, Rocío
Garde, José Julián
Soler, Ana Josefa
author_role author
author2 Sánchez Ajofrín, Irene
Peris Frau, Patricia
Maside Mielgo, Carolina
Montoro, Vidal
Fernández Santos, Rocío
Garde, José Julián
Soler, Ana Josefa
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Veterinaria
Sheep
Embryo
Oocyte
In vitro maturation
DNA damage
cAMP
IBMX
Forskolin
topic Veterinaria
Sheep
Embryo
Oocyte
In vitro maturation
DNA damage
cAMP
IBMX
Forskolin
description [EN] To date, the underlying mechanisms by which cAMP modulators act during in vitro maturation to improve oocyte developmental competence are poorly understood. Here, we sought to fill this knowledge gap by evaluating the use of phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and adenylyl cyclase activator forskolin during a culture period of 2 h before in vitro maturation (pre-IVM) on the nuclear and cytoplasmic maturation features in essential organelles, cumulus cells activity, and in vitro developmental potential of sheep oocytes. Results showed that pre-IVM treatment significantly decreased (p < 0.05) the DNA damage of mature oocytes (pre-IVM = 2.08% ± 3.51% vs. control = 20.58% ± 3.51%) and increased (p ≤ 0.05) expanded blastocyst rates compared to the control (from the total of oocytes: pre-IVM = 23.89% ± 1.47% vs. control = 18.22% ± 1.47%, and from the cleaved embryos: pre-IVM = 45.16% ± 1.73% vs. control = 32.88% ± 1.73%). Considering that oocytes are highly vulnerable to the accumulation of DNA damage because of exposure to in vitro culture conditions, our results suggest that the modulation of intra-oocyte cAMP levels with forskolin and IBMX before IVM might afford oocytes a more effective DNA repair mechanism to overcome damage obstacles and ultimately improve developmental competence. This previously unappreciated action of cAMP modulators could help to develop improved methods for assisted reproduction technologies in animal and clinical research.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.mdpi.com/2076-2615/11/9/2512
https://hdl.handle.net/10612/23608
url https://www.mdpi.com/2076-2615/11/9/2512
https://hdl.handle.net/10612/23608
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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