Effects of inactivated Mycobacterium bovis vaccination on molokai-origin wild pigs experimentally infected with virulent M. bovis

This article belongs to the Special Issue Tuberculosis Epidemiology and Control in Multi-Host Systems.

Detalles Bibliográficos
Autores: Nol, Pauline, Wehtje, Morgan E., Bowen, Richard A., Robbe-Austerman, Suelee, Thacker, Tyler C., Lantz, Kristina, Rhyan, Jack C., Baeten, Laurie A., Juste, Ramón A., Sevilla, Iker A., Gortázar, Christian, Vicente, Joaquín
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/221466
Acceso en línea:http://hdl.handle.net/10261/221466
Access Level:acceso abierto
Palabra clave:Sus scrofa
Wild pig
Feral swine
Mycobacterium bovis
Mycobacterium tuberculosis complex
Tuberculosis
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spelling Effects of inactivated Mycobacterium bovis vaccination on molokai-origin wild pigs experimentally infected with virulent M. bovisNol, PaulineWehtje, Morgan E.Bowen, Richard A.Robbe-Austerman, SueleeThacker, Tyler C.Lantz, KristinaRhyan, Jack C.Baeten, Laurie A.Juste, Ramón A.Sevilla, Iker A.Gortázar, ChristianVicente, JoaquínSus scrofaWild pigFeral swineMycobacterium bovisMycobacterium tuberculosis complexTuberculosisThis article belongs to the Special Issue Tuberculosis Epidemiology and Control in Multi-Host Systems.The wild pig population on Molokai, Hawaii, USA is a possible reservoir for bovine tuberculosis, caused by Mycobacterium bovis, and has been implicated in decades past as the source of disease for the island’s domestic cattle. Heat-inactivated vaccines have been effective for reducing disease prevalence in wild boar in Spain and could prove useful for managing M. bovis in Molokai wild pigs. We designed an experiment to test this vaccine in wild pigs of Molokai genetics. Fifteen 3–4-month-old pigs were orally administered 106–107 colony forming units (cfu) of heat-inactivated M. bovis (Vaccinates; n = 8; 0.2 mL) or phosphate buffered saline (Controls; n = 7; 0.2 mL). Each dose was administered in a 0.5 mL tube embedded in a fruit candy/cracked corn mix. Boosters were given seven weeks post-prime in the same manner and dose. Nineteen weeks post-prime, pigs were orally challenged with 1 × 106 cfu of virulent M. bovis. Twelve weeks post-challenge, pigs were euthanized and necropsied, at which time 23 different tissues from the head, thorax, and abdomen were collected and examined. Each tissue was assigned a lesion score. Ordinal lesion score data were analyzed using non-parametric Wilcoxon Signed Rank test. Effect size was calculated using Cohen’s d. Four of eight Vaccinates and four of seven Controls had gross and microscopic lesions, as well as culture-positive tissues. Vaccinates had statistically lower lesion scores than Controls in the following areas: gross thoracic lesion scores (p = 0.013 Cohen’s d = 0.33) and microscopic thoracic lesion scores (p = 0.002, Cohen’s d = 0.39). There were no differences in head lesion scores alone, both gross and microscopic, nor were there differences when comparing combined gross and microscopic head and thoracic lesion scores. These results are indicative that this vaccination protocol affords a modest degree of infection containment with this vaccine in Molokai wild pigs.This research was funded by United States Department of Agriculture, National Feral Swine Initiative.Peer reviewedMultidisciplinary Digital Publishing InstituteDepartment of Agriculture (US)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/221466reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/pathogens9030199Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2214662026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effects of inactivated Mycobacterium bovis vaccination on molokai-origin wild pigs experimentally infected with virulent M. bovis
title Effects of inactivated Mycobacterium bovis vaccination on molokai-origin wild pigs experimentally infected with virulent M. bovis
spellingShingle Effects of inactivated Mycobacterium bovis vaccination on molokai-origin wild pigs experimentally infected with virulent M. bovis
Nol, Pauline
Sus scrofa
Wild pig
Feral swine
Mycobacterium bovis
Mycobacterium tuberculosis complex
Tuberculosis
title_short Effects of inactivated Mycobacterium bovis vaccination on molokai-origin wild pigs experimentally infected with virulent M. bovis
title_full Effects of inactivated Mycobacterium bovis vaccination on molokai-origin wild pigs experimentally infected with virulent M. bovis
title_fullStr Effects of inactivated Mycobacterium bovis vaccination on molokai-origin wild pigs experimentally infected with virulent M. bovis
title_full_unstemmed Effects of inactivated Mycobacterium bovis vaccination on molokai-origin wild pigs experimentally infected with virulent M. bovis
title_sort Effects of inactivated Mycobacterium bovis vaccination on molokai-origin wild pigs experimentally infected with virulent M. bovis
dc.creator.none.fl_str_mv Nol, Pauline
Wehtje, Morgan E.
Bowen, Richard A.
Robbe-Austerman, Suelee
Thacker, Tyler C.
Lantz, Kristina
Rhyan, Jack C.
Baeten, Laurie A.
Juste, Ramón A.
Sevilla, Iker A.
Gortázar, Christian
Vicente, Joaquín
author Nol, Pauline
author_facet Nol, Pauline
Wehtje, Morgan E.
Bowen, Richard A.
Robbe-Austerman, Suelee
Thacker, Tyler C.
Lantz, Kristina
Rhyan, Jack C.
Baeten, Laurie A.
Juste, Ramón A.
Sevilla, Iker A.
Gortázar, Christian
Vicente, Joaquín
author_role author
author2 Wehtje, Morgan E.
Bowen, Richard A.
Robbe-Austerman, Suelee
Thacker, Tyler C.
Lantz, Kristina
Rhyan, Jack C.
Baeten, Laurie A.
Juste, Ramón A.
Sevilla, Iker A.
Gortázar, Christian
Vicente, Joaquín
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Department of Agriculture (US)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Sus scrofa
Wild pig
Feral swine
Mycobacterium bovis
Mycobacterium tuberculosis complex
Tuberculosis
topic Sus scrofa
Wild pig
Feral swine
Mycobacterium bovis
Mycobacterium tuberculosis complex
Tuberculosis
description This article belongs to the Special Issue Tuberculosis Epidemiology and Control in Multi-Host Systems.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/221466
url http://hdl.handle.net/10261/221466
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/pathogens9030199
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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