Decreased myocardial Titin expression in chronic alcoholic cardiomyopathy

Aims: Cardiomyopathy (CMP) with a reduced ejection fraction develops in a dose-28 dependent manner in one- third of subjects with a long-term history of heavy daily alcohol consumption. Ethanol alters heart transduction signals including excitation- contraction sarcomeric coupling, causing diastolic...

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Detalhes bibliográficos
Autores: Fernández-Solà, J. (Joaquim), Toll Argudo, Marta, Tobías, Ester, Moreno Lozano, Pedro Juan, Ferrer Curriu, Gemma, Guitart Mampel, Mariona, Planavila Porta, Ana, Garrabou Tornos, Glòria
Formato: artículo
Fecha de publicación:2018
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/125976
Acesso em linha:https://hdl.handle.net/2445/125976
Access Level:acceso abierto
Palavra-chave:Miocardi
Malalties cardiovasculars
Alcoholisme
Myocardium
Cardiovascular diseases
Alcoholism
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spelling Decreased myocardial Titin expression in chronic alcoholic cardiomyopathyFernández-Solà, J. (Joaquim)Toll Argudo, MartaTobías, EsterMoreno Lozano, Pedro JuanFerrer Curriu, GemmaGuitart Mampel, MarionaPlanavila Porta, AnaGarrabou Tornos, GlòriaMiocardiMalalties cardiovascularsAlcoholismeMyocardiumCardiovascular diseasesAlcoholismAims: Cardiomyopathy (CMP) with a reduced ejection fraction develops in a dose-28 dependent manner in one- third of subjects with a long-term history of heavy daily alcohol consumption. Ethanol alters heart transduction signals including excitation- contraction sarcomeric coupling, causing diastolic and systolic left-ventricular (LV) dysfunction. Titin is a giant structural sarcomeric filament macro protein involved in contractile heart function and contributes to cardiac myocyte elastic recoil, a key factor for diastolic LV filling. We evaluated whether titin expression is affected by chronic high-dose ethanol 35 consumption in alcoholic CMP. Methods and Results: We analyzed a total of 30 heart samples from human organ 37 donors: 20 from high alcohol consumers (10 without CMP and 10 with CMP) and 10 healthy controls. Patient evaluation comprised daily and lifetime ethanol consumption, chest X ray, 2-D echocardiography and LV histology. CMP was assessed by functional 40 and histological criteria. Titin activity was evaluated by specific immunohistochemical 41 (IHC) and transcript expression (rtPCR) assays. Titin IHC expression was clearly present in sarcomere areas of myocytes. Compared to healthy donors (82.58±3.36), alcohol consumers showed a significantly lower cardiac titin expression (71.29±3.16; 13.67±3.83% decrease; p=0.04), being significantly lower in alcohol consumers with CMP (62.31±4.18; 24.54±5.06% decrease, p<0.0009), compared to both their counter-parts without CMP (80.27±2.62; 2.80±3.17% decrease 47 vs. controls; p<0.0030 vs. alcoholic CMP). Titin transcript levels confirmed similar patterns of expression.InnovationInfo2018201820182018info:eu-repo/semantics/article8 p.application/pdfhttps://hdl.handle.net/2445/125976Articles publicats en revistes (Medicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésnullJournal of Cardiovascular Disease and Medicine, 2018, vol. 1, num. 1, p. 63-70, 2018info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1259762026-05-29T05:05:01Z
dc.title.none.fl_str_mv Decreased myocardial Titin expression in chronic alcoholic cardiomyopathy
title Decreased myocardial Titin expression in chronic alcoholic cardiomyopathy
spellingShingle Decreased myocardial Titin expression in chronic alcoholic cardiomyopathy
Fernández-Solà, J. (Joaquim)
Miocardi
Malalties cardiovasculars
Alcoholisme
Myocardium
Cardiovascular diseases
Alcoholism
title_short Decreased myocardial Titin expression in chronic alcoholic cardiomyopathy
title_full Decreased myocardial Titin expression in chronic alcoholic cardiomyopathy
title_fullStr Decreased myocardial Titin expression in chronic alcoholic cardiomyopathy
title_full_unstemmed Decreased myocardial Titin expression in chronic alcoholic cardiomyopathy
title_sort Decreased myocardial Titin expression in chronic alcoholic cardiomyopathy
dc.creator.none.fl_str_mv Fernández-Solà, J. (Joaquim)
Toll Argudo, Marta
Tobías, Ester
Moreno Lozano, Pedro Juan
Ferrer Curriu, Gemma
Guitart Mampel, Mariona
Planavila Porta, Ana
Garrabou Tornos, Glòria
author Fernández-Solà, J. (Joaquim)
author_facet Fernández-Solà, J. (Joaquim)
Toll Argudo, Marta
Tobías, Ester
Moreno Lozano, Pedro Juan
Ferrer Curriu, Gemma
Guitart Mampel, Mariona
Planavila Porta, Ana
Garrabou Tornos, Glòria
author_role author
author2 Toll Argudo, Marta
Tobías, Ester
Moreno Lozano, Pedro Juan
Ferrer Curriu, Gemma
Guitart Mampel, Mariona
Planavila Porta, Ana
Garrabou Tornos, Glòria
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Miocardi
Malalties cardiovasculars
Alcoholisme
Myocardium
Cardiovascular diseases
Alcoholism
topic Miocardi
Malalties cardiovasculars
Alcoholisme
Myocardium
Cardiovascular diseases
Alcoholism
description Aims: Cardiomyopathy (CMP) with a reduced ejection fraction develops in a dose-28 dependent manner in one- third of subjects with a long-term history of heavy daily alcohol consumption. Ethanol alters heart transduction signals including excitation- contraction sarcomeric coupling, causing diastolic and systolic left-ventricular (LV) dysfunction. Titin is a giant structural sarcomeric filament macro protein involved in contractile heart function and contributes to cardiac myocyte elastic recoil, a key factor for diastolic LV filling. We evaluated whether titin expression is affected by chronic high-dose ethanol 35 consumption in alcoholic CMP. Methods and Results: We analyzed a total of 30 heart samples from human organ 37 donors: 20 from high alcohol consumers (10 without CMP and 10 with CMP) and 10 healthy controls. Patient evaluation comprised daily and lifetime ethanol consumption, chest X ray, 2-D echocardiography and LV histology. CMP was assessed by functional 40 and histological criteria. Titin activity was evaluated by specific immunohistochemical 41 (IHC) and transcript expression (rtPCR) assays. Titin IHC expression was clearly present in sarcomere areas of myocytes. Compared to healthy donors (82.58±3.36), alcohol consumers showed a significantly lower cardiac titin expression (71.29±3.16; 13.67±3.83% decrease; p=0.04), being significantly lower in alcohol consumers with CMP (62.31±4.18; 24.54±5.06% decrease, p<0.0009), compared to both their counter-parts without CMP (80.27±2.62; 2.80±3.17% decrease 47 vs. controls; p<0.0030 vs. alcoholic CMP). Titin transcript levels confirmed similar patterns of expression.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/125976
url https://hdl.handle.net/2445/125976
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv null
Journal of Cardiovascular Disease and Medicine, 2018, vol. 1, num. 1, p. 63-70
dc.rights.none.fl_str_mv , 2018
info:eu-repo/semantics/openAccess
rights_invalid_str_mv , 2018
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8 p.
application/pdf
dc.publisher.none.fl_str_mv InnovationInfo
publisher.none.fl_str_mv InnovationInfo
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419527649886208
score 15.811543

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