Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell Lines

Colorectal cancer (CRC) is the fourth leading cause of cancer death in the world. Despite the screening programs, its incidence in the population below the 50s is increasing. Therefore, new stratification protocols based on multiparametric approaches are highly needed. In this scenario, the lipidome...

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Autores: Bestard-Escalas, Joan, Maimó-Barceló, Albert, Lopez, Daniel H, Reigada, Rebeca, Guardiola-Serrano, Francisca, Ramos-Vivas, Jose, Hornemann, Thorsten, Okazaki, Toshiro, Barcelo-Coblijn, Gwendolyn
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/22960
Acceso en línea:https://hdl.handle.net/20.500.12105/22960
Access Level:acceso abierto
Palabra clave:Colorectal cancer
Lipidomics
Lipid biomarkers
Extracellular vesicles
Cell lines
Plasmalogens
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spelling Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell LinesBestard-Escalas, JoanMaimó-Barceló, AlbertLopez, Daniel HReigada, RebecaGuardiola-Serrano, FranciscaRamos-Vivas, JoseHornemann, ThorstenOkazaki, ToshiroBarcelo-Coblijn, GwendolynColorectal cancerLipidomicsLipid biomarkersExtracellular vesiclesCell linesPlasmalogensColorectal cancer (CRC) is the fourth leading cause of cancer death in the world. Despite the screening programs, its incidence in the population below the 50s is increasing. Therefore, new stratification protocols based on multiparametric approaches are highly needed. In this scenario, the lipidome is emerging as a powerful tool to classify tumors, including CRC, wherein it has proven to be highly sensitive to cell malignization. Hence, the possibility to describe the lipidome at the level of lipid species has renewed the interest to investigate the role of specific lipid species in pathologic mechanisms, being commercial cell lines, a model still heavily used for this purpose. Herein, we characterize the membrane lipidome of five commercial colon cell lines and their extracellular vesicles (EVs). The results demonstrate that both cell and EVs lipidome was able to segregate cells according to their malignancy. Furthermore, all CRC lines shared a specific and strikingly homogenous impact on ether lipid species. Finally, this study also cautions about the need of being aware of the singularities of each cell line at the level of lipid species. Altogether, this study firmly lays the groundwork of using the lipidome as a solid source of tumor biomarkers.Multidisciplinary Digital Publishing Institute (MDPI)20242024-09-1320202020-05-0120202020-05-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttps://hdl.handle.net/20.500.12105/22960reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/229602026-06-12T12:43:37Z
dc.title.none.fl_str_mv Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell Lines
title Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell Lines
spellingShingle Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell Lines
Bestard-Escalas, Joan
Colorectal cancer
Lipidomics
Lipid biomarkers
Extracellular vesicles
Cell lines
Plasmalogens
title_short Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell Lines
title_full Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell Lines
title_fullStr Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell Lines
title_full_unstemmed Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell Lines
title_sort Common and Differential Traits of the Membrane Lipidome of Colon Cancer Cell Lines and Their Secreted Vesicles: Impact on Studies Using Cell Lines
dc.creator.none.fl_str_mv Bestard-Escalas, Joan
Maimó-Barceló, Albert
Lopez, Daniel H
Reigada, Rebeca
Guardiola-Serrano, Francisca
Ramos-Vivas, Jose
Hornemann, Thorsten
Okazaki, Toshiro
Barcelo-Coblijn, Gwendolyn
author Bestard-Escalas, Joan
author_facet Bestard-Escalas, Joan
Maimó-Barceló, Albert
Lopez, Daniel H
Reigada, Rebeca
Guardiola-Serrano, Francisca
Ramos-Vivas, Jose
Hornemann, Thorsten
Okazaki, Toshiro
Barcelo-Coblijn, Gwendolyn
author_role author
author2 Maimó-Barceló, Albert
Lopez, Daniel H
Reigada, Rebeca
Guardiola-Serrano, Francisca
Ramos-Vivas, Jose
Hornemann, Thorsten
Okazaki, Toshiro
Barcelo-Coblijn, Gwendolyn
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Colorectal cancer
Lipidomics
Lipid biomarkers
Extracellular vesicles
Cell lines
Plasmalogens
topic Colorectal cancer
Lipidomics
Lipid biomarkers
Extracellular vesicles
Cell lines
Plasmalogens
description Colorectal cancer (CRC) is the fourth leading cause of cancer death in the world. Despite the screening programs, its incidence in the population below the 50s is increasing. Therefore, new stratification protocols based on multiparametric approaches are highly needed. In this scenario, the lipidome is emerging as a powerful tool to classify tumors, including CRC, wherein it has proven to be highly sensitive to cell malignization. Hence, the possibility to describe the lipidome at the level of lipid species has renewed the interest to investigate the role of specific lipid species in pathologic mechanisms, being commercial cell lines, a model still heavily used for this purpose. Herein, we characterize the membrane lipidome of five commercial colon cell lines and their extracellular vesicles (EVs). The results demonstrate that both cell and EVs lipidome was able to segregate cells according to their malignancy. Furthermore, all CRC lines shared a specific and strikingly homogenous impact on ether lipid species. Finally, this study also cautions about the need of being aware of the singularities of each cell line at the level of lipid species. Altogether, this study firmly lays the groundwork of using the lipidome as a solid source of tumor biomarkers.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-05-01
2020
2020-05-01
2024
2024-09-13
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/22960
url https://hdl.handle.net/20.500.12105/22960
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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