Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata)

This study aimed to evaluate the effect of dietary protein source (fishmeal, FM; or plant-feedstuffs, PF) and dietary protein/carbohydrate (P/CH) ratio on gilthead seabream appetite regulation and intermediary metabolism. Additionally, the effect of sampling 5 h after feeding (AF) compared to 24 h A...

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Autores: Basto-Silva, Catarina, Enes, Paula, Oliva-Teles, Aires, Balbuena-Pecino, Sara, Navarro Álvarez, Isabel, Capilla Campos, Encarnación, Guerreiro, Inês
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/173707
Acceso en línea:https://hdl.handle.net/2445/173707
Access Level:acceso abierto
Palabra clave:Orada
Proteïnes
Expressió gènica
Sparus aurata
Proteins
Gene expression
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spelling Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata)Basto-Silva, CatarinaEnes, PaulaOliva-Teles, AiresBalbuena-Pecino, SaraNavarro Álvarez, IsabelCapilla Campos, EncarnaciónGuerreiro, InêsOradaProteïnesExpressió gènicaSparus aurataProteinsGene expressionThis study aimed to evaluate the effect of dietary protein source (fishmeal, FM; or plant-feedstuffs, PF) and dietary protein/carbohydrate (P/CH) ratio on gilthead seabream appetite regulation and intermediary metabolism. Additionally, the effect of sampling 5 h after feeding (AF) compared to 24 h AF was also evaluated. Four isolipidic diets were formulated having as major protein sources FM or PF (20% FM and 80% PF), and P/CH ratios of 50/10 or 40/20, being the pregelatinized maize starch the main carbohydrate source (diets FM-P50/CH10; FM-P40/CH20; PF-P50/CH10; PF-P40/CH20). Diets were fed until satiation to 140 g gilthead seabream for 41 days. The expression of appetite regulation genes was assessed at 5 and 24 h AF, while other evaluated parameters were assessed only at 5 h AF. Liver leptin expression was higher at 5 h AF, and brain leptin receptor (lepr) expression was higher at 24 h AF. Brain expression of cocaine- and amphetamine-regulated transcript (cart), leptin and ghrelin receptor (ghrr)-a and liver ghrr-b were also affected by sampling time, but the effects were dependent of the diet provided. FM-based diets promoted the expression of brain cart and leptin (at 24 h AF), and liver growth hormone receptor (ghr)-ii, and increased plasma cholesterol and total lipids levels. Fish fed the PF-based diets had higher liver glycogen content, number and size of adipocytes, and expression of hepatic leptin (at 24 h AF), fatty acid synthase, glucokinase, and target of rapamycin. Regarding dietary P/CH ratio, fish fed the P50/CH10 diets presented higher feed efficiency, plasma triglycerides, and expression of intestine cholecystokinin (at 5 h AF), liver ghrr-b (at 24 h AF), glutamate dehydrogenase and ghr-ii. The protein efficiency ratio, hepatosomatic and visceral indices, plasmatic glucose level, and brain lepr expression (at 5 h AF) were higher in fish fed the P40/CH20 diets. The majority of appetite regulation related-genes were not affected by the use of PF-based diets, while the higher dietary CH seemed to lead to a shorter satiety sensation. PF-based diets promoted liver lipid deposition, hypocholesterolemia, and the activation of glycogenesis pathway, while higher CH content induced an increase in plasma glucose that appeared to be stored as lipids. In conclusion, PF-based diets with up to 20% of CH can be used in gilthead seabream without compromising growth performance and FI, and only slightly modifying appetite and metabolic parameters.Elsevier B.V.2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/173707Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.aquaculture.2020.736142Aquaculture, 2020, vol. 533, p. 736142https://doi.org/10.1016/j.aquaculture.2020.736142cc-by-nc-nd (c) Elsevier B.V., 2020http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1737072026-05-27T06:46:51Z
dc.title.none.fl_str_mv Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata)
title Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata)
spellingShingle Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata)
Basto-Silva, Catarina
Orada
Proteïnes
Expressió gènica
Sparus aurata
Proteins
Gene expression
title_short Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata)
title_full Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata)
title_fullStr Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata)
title_full_unstemmed Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata)
title_sort Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata)
dc.creator.none.fl_str_mv Basto-Silva, Catarina
Enes, Paula
Oliva-Teles, Aires
Balbuena-Pecino, Sara
Navarro Álvarez, Isabel
Capilla Campos, Encarnación
Guerreiro, Inês
author Basto-Silva, Catarina
author_facet Basto-Silva, Catarina
Enes, Paula
Oliva-Teles, Aires
Balbuena-Pecino, Sara
Navarro Álvarez, Isabel
Capilla Campos, Encarnación
Guerreiro, Inês
author_role author
author2 Enes, Paula
Oliva-Teles, Aires
Balbuena-Pecino, Sara
Navarro Álvarez, Isabel
Capilla Campos, Encarnación
Guerreiro, Inês
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Orada
Proteïnes
Expressió gènica
Sparus aurata
Proteins
Gene expression
topic Orada
Proteïnes
Expressió gènica
Sparus aurata
Proteins
Gene expression
description This study aimed to evaluate the effect of dietary protein source (fishmeal, FM; or plant-feedstuffs, PF) and dietary protein/carbohydrate (P/CH) ratio on gilthead seabream appetite regulation and intermediary metabolism. Additionally, the effect of sampling 5 h after feeding (AF) compared to 24 h AF was also evaluated. Four isolipidic diets were formulated having as major protein sources FM or PF (20% FM and 80% PF), and P/CH ratios of 50/10 or 40/20, being the pregelatinized maize starch the main carbohydrate source (diets FM-P50/CH10; FM-P40/CH20; PF-P50/CH10; PF-P40/CH20). Diets were fed until satiation to 140 g gilthead seabream for 41 days. The expression of appetite regulation genes was assessed at 5 and 24 h AF, while other evaluated parameters were assessed only at 5 h AF. Liver leptin expression was higher at 5 h AF, and brain leptin receptor (lepr) expression was higher at 24 h AF. Brain expression of cocaine- and amphetamine-regulated transcript (cart), leptin and ghrelin receptor (ghrr)-a and liver ghrr-b were also affected by sampling time, but the effects were dependent of the diet provided. FM-based diets promoted the expression of brain cart and leptin (at 24 h AF), and liver growth hormone receptor (ghr)-ii, and increased plasma cholesterol and total lipids levels. Fish fed the PF-based diets had higher liver glycogen content, number and size of adipocytes, and expression of hepatic leptin (at 24 h AF), fatty acid synthase, glucokinase, and target of rapamycin. Regarding dietary P/CH ratio, fish fed the P50/CH10 diets presented higher feed efficiency, plasma triglycerides, and expression of intestine cholecystokinin (at 5 h AF), liver ghrr-b (at 24 h AF), glutamate dehydrogenase and ghr-ii. The protein efficiency ratio, hepatosomatic and visceral indices, plasmatic glucose level, and brain lepr expression (at 5 h AF) were higher in fish fed the P40/CH20 diets. The majority of appetite regulation related-genes were not affected by the use of PF-based diets, while the higher dietary CH seemed to lead to a shorter satiety sensation. PF-based diets promoted liver lipid deposition, hypocholesterolemia, and the activation of glycogenesis pathway, while higher CH content induced an increase in plasma glucose that appeared to be stored as lipids. In conclusion, PF-based diets with up to 20% of CH can be used in gilthead seabream without compromising growth performance and FI, and only slightly modifying appetite and metabolic parameters.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/173707
url https://hdl.handle.net/2445/173707
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.aquaculture.2020.736142
Aquaculture, 2020, vol. 533, p. 736142
https://doi.org/10.1016/j.aquaculture.2020.736142
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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