ZnO-mesoporous glass scaffolds loaded with osteostatin and mesenchymal cells improve bone healing in a rabbit bone defect.

The use of 3D scaffolds based on mesoporous bioactive glasses (MBG) enhanced with therapeutic ions, biomolecules and cells is emerging as a strategy to improve bone healing. In this paper, the osteogenic capability of ZnO-enriched MBG scaffolds loaded or not with osteostatin (OST) and human mesenchy...

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Authors: Lozano Borregón, Daniel, Gil Alvaroba, J, Heras Rubio, Clara, Sánchez Salcedo, Sandra, Gómez Palacio, V. E., Gómez Blasco, A, Doadrio Villarejo, Juan Carlos, Vallet Regí, María Dulce Nombre, Salinas Sánchez, Antonio Jesús
Format: article
Publication Date:2020
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/6596
Online Access:https://hdl.handle.net/20.500.14352/6596
Access Level:Open access
Keyword:66
546
615.46
Mesoporous bioactive glasses
ZnO
Osteostatin
In vivo evaluation
Bone regeneration
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
id ES_caaeb12013236db8f6d083f96e5d995c
oai_identifier_str oai:docta.ucm.es:20.500.14352/6596
network_acronym_str ES
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repository_id_str
spelling ZnO-mesoporous glass scaffolds loaded with osteostatin and mesenchymal cells improve bone healing in a rabbit bone defect.Lozano Borregón, DanielGil Alvaroba, JHeras Rubio, ClaraSánchez Salcedo, SandraGómez Palacio, V. E.Gómez Blasco, ADoadrio Villarejo, Juan CarlosVallet Regí, María Dulce NombreSalinas Sánchez, Antonio Jesús66546615.46Mesoporous bioactive glassesZnOOsteostatinIn vivo evaluationBone regenerationMaterialesQuímica inorgánica (Farmacia)3312 Tecnología de MaterialesThe use of 3D scaffolds based on mesoporous bioactive glasses (MBG) enhanced with therapeutic ions, biomolecules and cells is emerging as a strategy to improve bone healing. In this paper, the osteogenic capability of ZnO-enriched MBG scaffolds loaded or not with osteostatin (OST) and human mesenchymal stem cells (MSC) was evaluated after implantation in New Zealand rabbits. Cylindrical meso-macroporous scaffolds with composition (mol %) 82.2SiO2–10.3CaO–3.3P2O5–4.2ZnO (4ZN) were obtained by rapid prototyping and then, coated with gelatin for easy handling and potentiating the release of inorganic ions and OST. Bone defects (7.5 mm diameter, 12 mm depth) were drilled in the distal femoral epiphysis and filled with 4ZN, 4ZN+MSC, 4ZN+OST or 4ZN+MSC+OST materials to evaluate and compare their osteogenic features. Rabbits were sacrificed at 3 months extracting the distal third of bone specimens for necropsy, histological and microtomography (µCT) evaluations. Systems investigated exhibited bone regeneration capability. Thus, trabecular bone volume density (BV/TV) values obtained from µCT showed that the good bone healing capability of 4ZN was significantly improved by the scaffolds coated with OST and MSC. Our findings in vivo suggest the interest of these MBG complete systems to improve bone repair in the clinical practice.SpringerUniversidad Complutense de Madrid20202020-08-2520202020-08-25journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/6596reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/65962026-06-02T12:44:21Z
dc.title.none.fl_str_mv ZnO-mesoporous glass scaffolds loaded with osteostatin and mesenchymal cells improve bone healing in a rabbit bone defect.
title ZnO-mesoporous glass scaffolds loaded with osteostatin and mesenchymal cells improve bone healing in a rabbit bone defect.
spellingShingle ZnO-mesoporous glass scaffolds loaded with osteostatin and mesenchymal cells improve bone healing in a rabbit bone defect.
Lozano Borregón, Daniel
66
546
615.46
Mesoporous bioactive glasses
ZnO
Osteostatin
In vivo evaluation
Bone regeneration
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
title_short ZnO-mesoporous glass scaffolds loaded with osteostatin and mesenchymal cells improve bone healing in a rabbit bone defect.
title_full ZnO-mesoporous glass scaffolds loaded with osteostatin and mesenchymal cells improve bone healing in a rabbit bone defect.
title_fullStr ZnO-mesoporous glass scaffolds loaded with osteostatin and mesenchymal cells improve bone healing in a rabbit bone defect.
title_full_unstemmed ZnO-mesoporous glass scaffolds loaded with osteostatin and mesenchymal cells improve bone healing in a rabbit bone defect.
title_sort ZnO-mesoporous glass scaffolds loaded with osteostatin and mesenchymal cells improve bone healing in a rabbit bone defect.
dc.creator.none.fl_str_mv Lozano Borregón, Daniel
Gil Alvaroba, J
Heras Rubio, Clara
Sánchez Salcedo, Sandra
Gómez Palacio, V. E.
Gómez Blasco, A
Doadrio Villarejo, Juan Carlos
Vallet Regí, María Dulce Nombre
Salinas Sánchez, Antonio Jesús
author Lozano Borregón, Daniel
author_facet Lozano Borregón, Daniel
Gil Alvaroba, J
Heras Rubio, Clara
Sánchez Salcedo, Sandra
Gómez Palacio, V. E.
Gómez Blasco, A
Doadrio Villarejo, Juan Carlos
Vallet Regí, María Dulce Nombre
Salinas Sánchez, Antonio Jesús
author_role author
author2 Gil Alvaroba, J
Heras Rubio, Clara
Sánchez Salcedo, Sandra
Gómez Palacio, V. E.
Gómez Blasco, A
Doadrio Villarejo, Juan Carlos
Vallet Regí, María Dulce Nombre
Salinas Sánchez, Antonio Jesús
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 66
546
615.46
Mesoporous bioactive glasses
ZnO
Osteostatin
In vivo evaluation
Bone regeneration
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
topic 66
546
615.46
Mesoporous bioactive glasses
ZnO
Osteostatin
In vivo evaluation
Bone regeneration
Materiales
Química inorgánica (Farmacia)
3312 Tecnología de Materiales
description The use of 3D scaffolds based on mesoporous bioactive glasses (MBG) enhanced with therapeutic ions, biomolecules and cells is emerging as a strategy to improve bone healing. In this paper, the osteogenic capability of ZnO-enriched MBG scaffolds loaded or not with osteostatin (OST) and human mesenchymal stem cells (MSC) was evaluated after implantation in New Zealand rabbits. Cylindrical meso-macroporous scaffolds with composition (mol %) 82.2SiO2–10.3CaO–3.3P2O5–4.2ZnO (4ZN) were obtained by rapid prototyping and then, coated with gelatin for easy handling and potentiating the release of inorganic ions and OST. Bone defects (7.5 mm diameter, 12 mm depth) were drilled in the distal femoral epiphysis and filled with 4ZN, 4ZN+MSC, 4ZN+OST or 4ZN+MSC+OST materials to evaluate and compare their osteogenic features. Rabbits were sacrificed at 3 months extracting the distal third of bone specimens for necropsy, histological and microtomography (µCT) evaluations. Systems investigated exhibited bone regeneration capability. Thus, trabecular bone volume density (BV/TV) values obtained from µCT showed that the good bone healing capability of 4ZN was significantly improved by the scaffolds coated with OST and MSC. Our findings in vivo suggest the interest of these MBG complete systems to improve bone repair in the clinical practice.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-08-25
2020
2020-08-25
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/6596
url https://hdl.handle.net/20.500.14352/6596
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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