Synthetic and systems biology approaches towards the optimization of polyhydroxyalkanoates metabolism in Pseudomonas putida KT2440
Pseudomonas putida KT2440 is a model environmental bacterium attracting considerable interest as a cell-factory through synthetic and systems biology approaches. Its metabolism has been extensively characterized and a high-quality metabolic model is available (iJN1441), allowing for the systems eval...
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| Formato: | tesis doctoral |
| Fecha de publicación: | 2021 |
| País: | España |
| Recursos: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/11277 |
| Acesso em linha: | https://hdl.handle.net/20.500.14352/11277 |
| Access Level: | acceso abierto |
| Palavra-chave: | 579.8(043.2) Pseudomonas Microbiología (Farmacia) 3302.03 Microbiología Industrial |
| Resumo: | Pseudomonas putida KT2440 is a model environmental bacterium attracting considerable interest as a cell-factory through synthetic and systems biology approaches. Its metabolism has been extensively characterized and a high-quality metabolic model is available (iJN1441), allowing for the systems evaluation of its metabolic capabilities. Among its biotechnological applications, KT2440 is widely used for the production of polyhydroxyalkanoates (PHA), also known as bacterial polyesters or bioplastics. This family of polyesters presents interesting properties for replacing fossil fuel-based plastics. PHA metabolism in P. putida is mediated through the PHA polymerase (PhaC) and the PHA depolymerase (PhaZ) that synthesizes and degrades PHA, respectively, in a continuous cycle. The PHA are accumulated in the cytoplasm as carbon and energy stores, and contrary to oil-based plastic, they are produced from renewable resources. PHA metabolism goes beyond solely having a role in carbón storage since recent findings have highlighted its function as a balancing route that increases P. putida fitness during environmental perturbations... |
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