Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome

The emergence of pathogenic strains resistant to multiple antimicrobials is a pressing problem in modern healthcare. Antimicrobial resistance is mediated primarily by dissemination of resistance determinants via horizontal gene transfer. The dissemination of some resistance genes has been well docum...

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Autores: Sánchez Osuna, Miquel|||0000-0001-5267-4814, Barbé García, Jordi|||0000-0003-4408-013X, Erill, Ivan|||0000-0002-7280-7191
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:273067
Acceso en línea:https://ddd.uab.cat/record/273067
https://dx.doi.org/urn:doi:10.3390/antibiotics12020281
Access Level:acceso abierto
Palabra clave:Antimicrobial resistance
Dissemination
Evolution
Plasmid
Mobile genetic element
GC content
Horizontal gene transfer
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spelling Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global PlasmidomeSánchez Osuna, Miquel|||0000-0001-5267-4814Barbé García, Jordi|||0000-0003-4408-013XErill, Ivan|||0000-0002-7280-7191Antimicrobial resistanceDisseminationEvolutionPlasmidMobile genetic elementGC contentHorizontal gene transferThe emergence of pathogenic strains resistant to multiple antimicrobials is a pressing problem in modern healthcare. Antimicrobial resistance is mediated primarily by dissemination of resistance determinants via horizontal gene transfer. The dissemination of some resistance genes has been well documented, but few studies have analyzed the patterns underpinning the dissemination of antimicrobial resistance genes. Analyzing the %GC content of plasmid-borne antimicrobial resistance genes relative to their host genome %GC content provides a means to efficiently detect and quantify dissemination of antimicrobial resistance genes. In this work we automate %GC content analysis to perform a comprehensive analysis of known antimicrobial resistance genes in publicly available plasmid sequences. We find that the degree to which antimicrobial resistance genes are disseminated depends primarily on the resistance mechanism. Our analysis identifies conjugative plasmids as primary dissemination vectors and indicates that most broadly disseminated genes have spread from single genomic backgrounds. We show that resistance dissemination profiles vary greatly among antimicrobials, oftentimes reflecting stewardship measures. Our findings establish %GC content analysis as a powerful, intuitive and scalable method to monitor the dissemination of resistance determinants using publicly available sequence data. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/273067https://dx.doi.org/urn:doi:10.3390/antibiotics12020281reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2730672026-06-06T12:50:31Z
dc.title.none.fl_str_mv Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
title Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
spellingShingle Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
Sánchez Osuna, Miquel|||0000-0001-5267-4814
Antimicrobial resistance
Dissemination
Evolution
Plasmid
Mobile genetic element
GC content
Horizontal gene transfer
title_short Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
title_full Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
title_fullStr Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
title_full_unstemmed Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
title_sort Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
dc.creator.none.fl_str_mv Sánchez Osuna, Miquel|||0000-0001-5267-4814
Barbé García, Jordi|||0000-0003-4408-013X
Erill, Ivan|||0000-0002-7280-7191
author Sánchez Osuna, Miquel|||0000-0001-5267-4814
author_facet Sánchez Osuna, Miquel|||0000-0001-5267-4814
Barbé García, Jordi|||0000-0003-4408-013X
Erill, Ivan|||0000-0002-7280-7191
author_role author
author2 Barbé García, Jordi|||0000-0003-4408-013X
Erill, Ivan|||0000-0002-7280-7191
author2_role author
author
dc.subject.none.fl_str_mv Antimicrobial resistance
Dissemination
Evolution
Plasmid
Mobile genetic element
GC content
Horizontal gene transfer
topic Antimicrobial resistance
Dissemination
Evolution
Plasmid
Mobile genetic element
GC content
Horizontal gene transfer
description The emergence of pathogenic strains resistant to multiple antimicrobials is a pressing problem in modern healthcare. Antimicrobial resistance is mediated primarily by dissemination of resistance determinants via horizontal gene transfer. The dissemination of some resistance genes has been well documented, but few studies have analyzed the patterns underpinning the dissemination of antimicrobial resistance genes. Analyzing the %GC content of plasmid-borne antimicrobial resistance genes relative to their host genome %GC content provides a means to efficiently detect and quantify dissemination of antimicrobial resistance genes. In this work we automate %GC content analysis to perform a comprehensive analysis of known antimicrobial resistance genes in publicly available plasmid sequences. We find that the degree to which antimicrobial resistance genes are disseminated depends primarily on the resistance mechanism. Our analysis identifies conjugative plasmids as primary dissemination vectors and indicates that most broadly disseminated genes have spread from single genomic backgrounds. We show that resistance dissemination profiles vary greatly among antimicrobials, oftentimes reflecting stewardship measures. Our findings establish %GC content analysis as a powerful, intuitive and scalable method to monitor the dissemination of resistance determinants using publicly available sequence data.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/273067
https://dx.doi.org/urn:doi:10.3390/antibiotics12020281
url https://ddd.uab.cat/record/273067
https://dx.doi.org/urn:doi:10.3390/antibiotics12020281
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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