NAPPA as a real new method for protein microarray generation

This is an open access article distributed under the Creative Commons Attribution License.-- This article belongs to the Special Issue "New and Old Technologies for Generation of Microarrays".

Detalles Bibliográficos
Autores: Díez, Paula, González González, María, Lourido, Lucía, Dégano, Rosa M., Ibarrola, Nieves, Casado-Vela, Juan, LaBaer, Joshua, Fuentes, Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/122794
Acceso en línea:http://hdl.handle.net/10261/122794
Access Level:acceso abierto
Palabra clave:Protein microarray
NAPPA
High-throughput screening
Biomarkers
Protein-protein interaction
Microarray generation
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spelling NAPPA as a real new method for protein microarray generationDíez, PaulaGonzález González, MaríaLourido, LucíaDégano, Rosa M.Ibarrola, NievesCasado-Vela, JuanLaBaer, JoshuaFuentes, ManuelProtein microarrayNAPPAHigh-throughput screeningBiomarkersProtein-protein interactionMicroarray generationThis is an open access article distributed under the Creative Commons Attribution License.-- This article belongs to the Special Issue "New and Old Technologies for Generation of Microarrays".Nucleic Acid Programmable Protein Arrays (NAPPA) have emerged as a powerful and innovative technology for the screening of biomarkers and the study of protein-protein interactions, among others possible applications. The principal advantages are the high specificity and sensitivity that this platform offers. Moreover, compared to conventional protein microarrays, NAPPA technology avoids the necessity of protein purification, which is expensive and time-consuming, by substituting expression in situ with an in vitro transcription/translation kit. In summary, NAPPA arrays have been broadly employed in different studies improving knowledge about diseases and responses to treatments. Here, we review the principal advances and applications performed using this platform during the last years.We gratefully acknowledge financial support from the Carlos III Health Institute of Spain (ISCIII, FIS PI14/01538)-Fondos FEDER (EU). The Proteomics Unit belongs to ProteoRed, PRB2-ISCIII, supported by grant PT13/0001. P.D. is supported by a JCYL-EDU/346/2013 PhD scholarship.We acknowledge the support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).Peer reviewedMultidisciplinary Digital Publishing InstituteInstituto de Salud Carlos IIIJunta de Castilla y LeónCSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/122794reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/microarrays4020214Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1227942026-05-22T06:33:51Z
dc.title.none.fl_str_mv NAPPA as a real new method for protein microarray generation
title NAPPA as a real new method for protein microarray generation
spellingShingle NAPPA as a real new method for protein microarray generation
Díez, Paula
Protein microarray
NAPPA
High-throughput screening
Biomarkers
Protein-protein interaction
Microarray generation
title_short NAPPA as a real new method for protein microarray generation
title_full NAPPA as a real new method for protein microarray generation
title_fullStr NAPPA as a real new method for protein microarray generation
title_full_unstemmed NAPPA as a real new method for protein microarray generation
title_sort NAPPA as a real new method for protein microarray generation
dc.creator.none.fl_str_mv Díez, Paula
González González, María
Lourido, Lucía
Dégano, Rosa M.
Ibarrola, Nieves
Casado-Vela, Juan
LaBaer, Joshua
Fuentes, Manuel
author Díez, Paula
author_facet Díez, Paula
González González, María
Lourido, Lucía
Dégano, Rosa M.
Ibarrola, Nieves
Casado-Vela, Juan
LaBaer, Joshua
Fuentes, Manuel
author_role author
author2 González González, María
Lourido, Lucía
Dégano, Rosa M.
Ibarrola, Nieves
Casado-Vela, Juan
LaBaer, Joshua
Fuentes, Manuel
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Junta de Castilla y León
CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Protein microarray
NAPPA
High-throughput screening
Biomarkers
Protein-protein interaction
Microarray generation
topic Protein microarray
NAPPA
High-throughput screening
Biomarkers
Protein-protein interaction
Microarray generation
description This is an open access article distributed under the Creative Commons Attribution License.-- This article belongs to the Special Issue "New and Old Technologies for Generation of Microarrays".
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/122794
url http://hdl.handle.net/10261/122794
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/microarrays4020214
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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