Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata).

Amri, Afef; Bouraoui, Zied; Balbuena-Pecino, Sara; Capilla Campos, Encarnación; Gharred, Tahar; Haouas, Zohra; Guerbej, Hamadi; Hosni, Karim; Navarro Álvarez, Isabel; Jebali, Jamel

Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata).

This study aimed to assess the effects of dietary increasing concentrations of Aloe vera (AV) powder of 0.5%, 2.5% and 5% on the growth performance, hepatic oxidative status, histology, and lipid metabolism and cellular signaling pathways-related genes' expression in gilthead sea bream (Sparus...

Descripción completa

Detalles Bibliográficos
Autores:	Amri, Afef, Bouraoui, Zied, Balbuena-Pecino, Sara, Capilla Campos, Encarnación, Gharred, Tahar, Haouas, Zohra, Guerbej, Hamadi, Hosni, Karim, Navarro Álvarez, Isabel, Jebali, Jamel
Tipo de recurso:	artículo
Estado:	Versión aceptada para publicación
Fecha de publicación:	2022
País:	España
Institución:	Universidad de Barcelona
Repositorio:	Dipòsit Digital de la UB
OAI Identifier:	oai:diposit.ub.edu:2445/186450
Acceso en línea:	https://hdl.handle.net/2445/186450
Access Level:	acceso abierto
Palabra clave:	Àloes Orada Metabolisme dels lípids Estrès oxidatiu Expressió gènica Aloe Sparus aurata Lipid metabolism Oxidative stress Gene expression

id	ES_ca3f2abe2e8f46476d04b19e8ca6c565
oai_identifier_str	oai:diposit.ub.edu:2445/186450
network_acronym_str	ES
network_name_str	España
repository_id_str
spelling	Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata).Amri, AfefBouraoui, ZiedBalbuena-Pecino, SaraCapilla Campos, EncarnaciónGharred, TaharHaouas, ZohraGuerbej, HamadiHosni, KarimNavarro Álvarez, IsabelJebali, JamelÀloesOradaMetabolisme dels lípidsEstrès oxidatiuExpressió gènicaAloeSparus aurataLipid metabolismOxidative stressGene expressionThis study aimed to assess the effects of dietary increasing concentrations of Aloe vera (AV) powder of 0.5%, 2.5% and 5% on the growth performance, hepatic oxidative status, histology, and lipid metabolism and cellular signaling pathways-related genes' expression in gilthead sea bream (Sparus aurata). The preliminary phytochemical analysis revealed the richness of the dried AV extract on total phenol content, total flavonoid content, and condensed tannins when compared to the lyophilized sample. The dried extract showed a good DPPH-radical scavenging activity and its profiling by HPLC-DAD-ESI-MS revealed the presence of anthraquinones namely aloin A, aloin B and their hydroxyl (7-hydroxyaloin A and 7-hydroxyaloin B) and methyl-hydroxy (8-O-methyl-7-hydroxyaloin A and 8-O-methyl-7-hydroxyaloin B) derivatives as well as aloeresin A and B. The AV supplementation in fish diet did not affect growth performance (WG, WGR, and SGR) and feed utilization (FI, FCR, FER), and HSI indexes. However, the hepatic insulin-like growth factors (IGF-I and II) levels were significantly enhanced. Genes' expression levels of enzymes or transcription factors involved in lipolysis (lpl, hsl, and atgl), beta-oxidation (pparα, hadh), fatty acid transporters (cd36, fabp11) and lxrα were significantly down-regulated by the two high concentrations of AV powder. In contrast, fatty acid synthase (fas), a key gene of lipogenesis was significantly up regulated by dietary AV 5% powder supplementation. The induction of fas together with the down-regulation of peroxisome proliferator-activated receptor (pparα) and hydroxyacyl-coenzyme A dehydrogenase (hadh) could explain the lipid accumulation resulting in hepatic steatosis, which was confirmed by histological analysis, since the diets at the two higher concentrations (AV 2.5% and AV 5%) induced a significant increase in the number and diameter of hepatic lipid vacuoles in a dose dependent manner. Moreover, the mRNA levels of protein kinase B named (akt), mammalian target of rapamycin (mtor) and extracellular regulated kinase (erk1/2) involved in cell survival and proliferation were decreased by all AV powder supplemented diets. AV 5% increased catalase and glutathione S transferase activities suggesting a cellular strategy to fight against reactive oxygen species (ROS) accumulation. In conclusion, dietary supplementation with AV 0.5% is recommended for gilthead sea bream feed formulation, as it stimulates the igf-i expression. However, higher levels of AV should be avoided as they might cause lipid metabolism disruption, oxidative stress and liver steatosis.Elsevier B.V.2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/186450Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.aquaculture.2022.738433Aquaculture, 2022, vol. 559, num. 738433https://doi.org/10.1016/j.aquaculture.2022.738433cc-by-nc-nd (c) Elsevier B.V., 2022https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1864502026-05-27T06:46:51Z
dc.title.none.fl_str_mv	Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata).
title	Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata).
spellingShingle	Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata). Amri, Afef Àloes Orada Metabolisme dels lípids Estrès oxidatiu Expressió gènica Aloe Sparus aurata Lipid metabolism Oxidative stress Gene expression
title_short	Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata).
title_full	Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata).
title_fullStr	Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata).
title_full_unstemmed	Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata).
title_sort	Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata).
dc.creator.none.fl_str_mv	Amri, Afef Bouraoui, Zied Balbuena-Pecino, Sara Capilla Campos, Encarnación Gharred, Tahar Haouas, Zohra Guerbej, Hamadi Hosni, Karim Navarro Álvarez, Isabel Jebali, Jamel
author	Amri, Afef
author_facet	Amri, Afef Bouraoui, Zied Balbuena-Pecino, Sara Capilla Campos, Encarnación Gharred, Tahar Haouas, Zohra Guerbej, Hamadi Hosni, Karim Navarro Álvarez, Isabel Jebali, Jamel
author_role	author
author2	Bouraoui, Zied Balbuena-Pecino, Sara Capilla Campos, Encarnación Gharred, Tahar Haouas, Zohra Guerbej, Hamadi Hosni, Karim Navarro Álvarez, Isabel Jebali, Jamel
author2_role	author author author author author author author author author
dc.subject.none.fl_str_mv	Àloes Orada Metabolisme dels lípids Estrès oxidatiu Expressió gènica Aloe Sparus aurata Lipid metabolism Oxidative stress Gene expression
topic	Àloes Orada Metabolisme dels lípids Estrès oxidatiu Expressió gènica Aloe Sparus aurata Lipid metabolism Oxidative stress Gene expression
description	This study aimed to assess the effects of dietary increasing concentrations of Aloe vera (AV) powder of 0.5%, 2.5% and 5% on the growth performance, hepatic oxidative status, histology, and lipid metabolism and cellular signaling pathways-related genes' expression in gilthead sea bream (Sparus aurata). The preliminary phytochemical analysis revealed the richness of the dried AV extract on total phenol content, total flavonoid content, and condensed tannins when compared to the lyophilized sample. The dried extract showed a good DPPH-radical scavenging activity and its profiling by HPLC-DAD-ESI-MS revealed the presence of anthraquinones namely aloin A, aloin B and their hydroxyl (7-hydroxyaloin A and 7-hydroxyaloin B) and methyl-hydroxy (8-O-methyl-7-hydroxyaloin A and 8-O-methyl-7-hydroxyaloin B) derivatives as well as aloeresin A and B. The AV supplementation in fish diet did not affect growth performance (WG, WGR, and SGR) and feed utilization (FI, FCR, FER), and HSI indexes. However, the hepatic insulin-like growth factors (IGF-I and II) levels were significantly enhanced. Genes' expression levels of enzymes or transcription factors involved in lipolysis (lpl, hsl, and atgl), beta-oxidation (pparα, hadh), fatty acid transporters (cd36, fabp11) and lxrα were significantly down-regulated by the two high concentrations of AV powder. In contrast, fatty acid synthase (fas), a key gene of lipogenesis was significantly up regulated by dietary AV 5% powder supplementation. The induction of fas together with the down-regulation of peroxisome proliferator-activated receptor (pparα) and hydroxyacyl-coenzyme A dehydrogenase (hadh) could explain the lipid accumulation resulting in hepatic steatosis, which was confirmed by histological analysis, since the diets at the two higher concentrations (AV 2.5% and AV 5%) induced a significant increase in the number and diameter of hepatic lipid vacuoles in a dose dependent manner. Moreover, the mRNA levels of protein kinase B named (akt), mammalian target of rapamycin (mtor) and extracellular regulated kinase (erk1/2) involved in cell survival and proliferation were decreased by all AV powder supplemented diets. AV 5% increased catalase and glutathione S transferase activities suggesting a cellular strategy to fight against reactive oxygen species (ROS) accumulation. In conclusion, dietary supplementation with AV 0.5% is recommended for gilthead sea bream feed formulation, as it stimulates the igf-i expression. However, higher levels of AV should be avoided as they might cause lipid metabolism disruption, oxidative stress and liver steatosis.
publishDate	2022
dc.date.none.fl_str_mv	2022
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion
format	article
status_str	acceptedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/186450
url	https://hdl.handle.net/2445/186450
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Versió postprint del document publicat a: https://doi.org/10.1016/j.aquaculture.2022.738433 Aquaculture, 2022, vol. 559, num. 738433 https://doi.org/10.1016/j.aquaculture.2022.738433
dc.rights.none.fl_str_mv	cc-by-nc-nd (c) Elsevier B.V., 2022 https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc-by-nc-nd (c) Elsevier B.V., 2022 https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Elsevier B.V.
publisher.none.fl_str_mv	Elsevier B.V.
dc.source.none.fl_str_mv	Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona
instname_str	Universidad de Barcelona
reponame_str	Dipòsit Digital de la UB
collection	Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_	1869419463331282944
score	15.300719

Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata).

Similares en LA Referencia