Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips

Atomic force microscopy (AFM) allows the acquisition of high-resolution images and the measurement of mechanical properties of living cells under physiological conditions. AFM cantilevers with blunted pyramidal tips are commonly used to obtain images of living cells. Measurement of mechanical proper...

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Detalles Bibliográficos
Autores: Rico Camps, Félix, Roca-Cusachs Soulere, Pere, Gavara i Casas, Núria, Farré Ventura, Ramon, Rotger i Estapé, Maria del Mar, Navajas Navarro, Daniel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/18686
Acceso en línea:https://hdl.handle.net/2445/18686
Access Level:acceso abierto
Palabra clave:Microscòpia de força atòmica
Biofísica
Cèl·lules
Atomic force microscopy
Biophysics
Cells
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spelling Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tipsRico Camps, FélixRoca-Cusachs Soulere, PereGavara i Casas, NúriaFarré Ventura, RamonRotger i Estapé, Maria del MarNavajas Navarro, DanielMicroscòpia de força atòmicaBiofísicaCèl·lulesAtomic force microscopyBiophysicsCellsAtomic force microscopy (AFM) allows the acquisition of high-resolution images and the measurement of mechanical properties of living cells under physiological conditions. AFM cantilevers with blunted pyramidal tips are commonly used to obtain images of living cells. Measurement of mechanical properties with these tips requires a contact model that takes into account their blunted geometry. The aim of this work was to develop a contact model of a blunted pyramidal tip and to assess the suitability of pyramidal tips for probing mechanical properties of soft gels and living cells. We developed a contact model of a blunted pyramidal tip indenting an elastic half-space. We measured Young’s modulus ( E ) and the complex shear modulus ( G ∗ = G ′ + i G ′′ ) of agarose gels and A549 alveolar epithelial cells with pyramidal tips and compared them with those obtained with spherical tips. The gels exhibited an elastic behavior with almost coincident loading and unloading force curves and negligible values of G ′′ . E fell sharply with indentation up to ∼ 300 nm , showing a linear regime for deeper indentations. A similar indentation dependence of E with twofold lower values at the linear regime was obtained with the spherical tip fitted with Hertz’s model. The dependence of E on indentation in cells paralleled that found in gels. Cells exhibited viscoelastic behavior with G ′′ / G ′ ∼ 1 / 4 . Pyramidal tips commonly used for AFM imaging are suitable for probing mechanical properties of soft gels and living cells.The American Physical Society201120112005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfhttps://hdl.handle.net/2445/18686Articles publicats en revistes (Ciències Fisiològiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció digital del document publicat a: http://dx.doi.org/10.1103/PhysRevE.72.021914Physical Review E, 2005, vol. 72, núm. 2, p. 021914-1 - 021914-10http://dx.doi.org/10.1103/PhysRevE.72.021914(c) The American Physical Society, 2005info:eu-repo/semantics/openAccessoai:recercat.cat:2445/186862026-05-29T05:05:01Z
dc.title.none.fl_str_mv Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips
title Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips
spellingShingle Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips
Rico Camps, Félix
Microscòpia de força atòmica
Biofísica
Cèl·lules
Atomic force microscopy
Biophysics
Cells
title_short Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips
title_full Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips
title_fullStr Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips
title_full_unstemmed Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips
title_sort Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips
dc.creator.none.fl_str_mv Rico Camps, Félix
Roca-Cusachs Soulere, Pere
Gavara i Casas, Núria
Farré Ventura, Ramon
Rotger i Estapé, Maria del Mar
Navajas Navarro, Daniel
author Rico Camps, Félix
author_facet Rico Camps, Félix
Roca-Cusachs Soulere, Pere
Gavara i Casas, Núria
Farré Ventura, Ramon
Rotger i Estapé, Maria del Mar
Navajas Navarro, Daniel
author_role author
author2 Roca-Cusachs Soulere, Pere
Gavara i Casas, Núria
Farré Ventura, Ramon
Rotger i Estapé, Maria del Mar
Navajas Navarro, Daniel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Microscòpia de força atòmica
Biofísica
Cèl·lules
Atomic force microscopy
Biophysics
Cells
topic Microscòpia de força atòmica
Biofísica
Cèl·lules
Atomic force microscopy
Biophysics
Cells
description Atomic force microscopy (AFM) allows the acquisition of high-resolution images and the measurement of mechanical properties of living cells under physiological conditions. AFM cantilevers with blunted pyramidal tips are commonly used to obtain images of living cells. Measurement of mechanical properties with these tips requires a contact model that takes into account their blunted geometry. The aim of this work was to develop a contact model of a blunted pyramidal tip and to assess the suitability of pyramidal tips for probing mechanical properties of soft gels and living cells. We developed a contact model of a blunted pyramidal tip indenting an elastic half-space. We measured Young’s modulus ( E ) and the complex shear modulus ( G ∗ = G ′ + i G ′′ ) of agarose gels and A549 alveolar epithelial cells with pyramidal tips and compared them with those obtained with spherical tips. The gels exhibited an elastic behavior with almost coincident loading and unloading force curves and negligible values of G ′′ . E fell sharply with indentation up to ∼ 300 nm , showing a linear regime for deeper indentations. A similar indentation dependence of E with twofold lower values at the linear regime was obtained with the spherical tip fitted with Hertz’s model. The dependence of E on indentation in cells paralleled that found in gels. Cells exhibited viscoelastic behavior with G ′′ / G ′ ∼ 1 / 4 . Pyramidal tips commonly used for AFM imaging are suitable for probing mechanical properties of soft gels and living cells.
publishDate 2005
dc.date.none.fl_str_mv 2005
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/18686
url https://hdl.handle.net/2445/18686
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció digital del document publicat a: http://dx.doi.org/10.1103/PhysRevE.72.021914
Physical Review E, 2005, vol. 72, núm. 2, p. 021914-1 - 021914-10
http://dx.doi.org/10.1103/PhysRevE.72.021914
dc.rights.none.fl_str_mv (c) The American Physical Society, 2005
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) The American Physical Society, 2005
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv The American Physical Society
publisher.none.fl_str_mv The American Physical Society
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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