Membrane protein stabilization strategies for structural and functional studies

Accounting for nearly two-thirds of known druggable targets, membrane proteins are highly relevant for cell physiology and pharmacology. In this regard, the structural determination of pharmacologically relevant targets would facilitate the intelligent design of new drugs. The structural biology of...

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Detalhes bibliográficos
Autores: Errasti-Murugarren, Ekaitz, Bartoccioni, Paola, Palacín Prieto, Manuel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/191295
Acesso em linha:https://hdl.handle.net/2445/191295
Access Level:acceso abierto
Palavra-chave:Proteïnes de membrana
Mutagènesi
Detergents
Lípids
Membrane proteins
Mutagenesis
Lipids
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spelling Membrane protein stabilization strategies for structural and functional studiesErrasti-Murugarren, EkaitzBartoccioni, PaolaPalacín Prieto, ManuelProteïnes de membranaMutagènesiDetergentsLípidsMembrane proteinsMutagenesisDetergentsLipidsAccounting for nearly two-thirds of known druggable targets, membrane proteins are highly relevant for cell physiology and pharmacology. In this regard, the structural determination of pharmacologically relevant targets would facilitate the intelligent design of new drugs. The structural biology of membrane proteins is a field experiencing significant growth as a result of the development of new strategies for structure determination. However, membrane protein preparation for structural studies continues to be a limiting step in many cases due to the inherent instability of these molecules in non-native membrane environments. This review describes the approaches that have been developed to improve membrane protein stability. Membrane protein mutagenesis, detergent selection, lipid membrane mimics, antibodies, and ligands are described in this review as approaches to facilitate the production of purified and stable membrane proteins of interest for structural and functional studies.MDPI2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/191295Articles publicats en revistes (Ciències Fisiològiques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/membranes11020155Membranes, 2021, vol. 11, num. 2https://doi.org/10.3390/membranes11020155cc-by (c) Errasti Murugarren, Ekaitz et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1912952026-05-27T06:46:51Z
dc.title.none.fl_str_mv Membrane protein stabilization strategies for structural and functional studies
title Membrane protein stabilization strategies for structural and functional studies
spellingShingle Membrane protein stabilization strategies for structural and functional studies
Errasti-Murugarren, Ekaitz
Proteïnes de membrana
Mutagènesi
Detergents
Lípids
Membrane proteins
Mutagenesis
Detergents
Lipids
title_short Membrane protein stabilization strategies for structural and functional studies
title_full Membrane protein stabilization strategies for structural and functional studies
title_fullStr Membrane protein stabilization strategies for structural and functional studies
title_full_unstemmed Membrane protein stabilization strategies for structural and functional studies
title_sort Membrane protein stabilization strategies for structural and functional studies
dc.creator.none.fl_str_mv Errasti-Murugarren, Ekaitz
Bartoccioni, Paola
Palacín Prieto, Manuel
author Errasti-Murugarren, Ekaitz
author_facet Errasti-Murugarren, Ekaitz
Bartoccioni, Paola
Palacín Prieto, Manuel
author_role author
author2 Bartoccioni, Paola
Palacín Prieto, Manuel
author2_role author
author
dc.subject.none.fl_str_mv Proteïnes de membrana
Mutagènesi
Detergents
Lípids
Membrane proteins
Mutagenesis
Detergents
Lipids
topic Proteïnes de membrana
Mutagènesi
Detergents
Lípids
Membrane proteins
Mutagenesis
Detergents
Lipids
description Accounting for nearly two-thirds of known druggable targets, membrane proteins are highly relevant for cell physiology and pharmacology. In this regard, the structural determination of pharmacologically relevant targets would facilitate the intelligent design of new drugs. The structural biology of membrane proteins is a field experiencing significant growth as a result of the development of new strategies for structure determination. However, membrane protein preparation for structural studies continues to be a limiting step in many cases due to the inherent instability of these molecules in non-native membrane environments. This review describes the approaches that have been developed to improve membrane protein stability. Membrane protein mutagenesis, detergent selection, lipid membrane mimics, antibodies, and ligands are described in this review as approaches to facilitate the production of purified and stable membrane proteins of interest for structural and functional studies.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/191295
url https://hdl.handle.net/2445/191295
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/membranes11020155
Membranes, 2021, vol. 11, num. 2
https://doi.org/10.3390/membranes11020155
dc.rights.none.fl_str_mv cc-by (c) Errasti Murugarren, Ekaitz et al., 2021
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Errasti Murugarren, Ekaitz et al., 2021
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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