Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.

Natural killer (NK) cells recognize and kill target cells undergoing different types of stress. NK cells are also capable of modulating immune responses. In particular, they regulate T cell functions. Small RNA next-generation sequencing of resting and activated human NK cells and their secreted ext...

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Autores: Dosil, Sara G, Lopez-Cobo, Sheila, Rodriguez-Galan, Ana, Fernandez-Delgado, Irene, Ramirez-Huesca, Marta, Milan-Rois, Paula, Castellanos, Milagros, Somoza, Alvaro, Gómez, Manuel José, Reyburn, Hugh T, Vales-Gomez, Mar, Sánchez Madrid, Francisco, Fernandez-Messina, Lola
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/15670
Acceso en línea:http://hdl.handle.net/20.500.12105/15670
Access Level:acceso abierto
Palabra clave:Extracellular Vesicles
MicroRNAs
Animals
Humans
Killer Cells, Natural
Mice
RNA, Messenger
T-Lymphocytes
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spelling Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.Dosil, Sara GLopez-Cobo, SheilaRodriguez-Galan, AnaFernandez-Delgado, IreneRamirez-Huesca, MartaMilan-Rois, PaulaCastellanos, MilagrosSomoza, AlvaroGómez, Manuel JoséReyburn, Hugh TVales-Gomez, MarSánchez Madrid, FranciscoFernandez-Messina, LolaExtracellular VesiclesMicroRNAsAnimalsHumansKiller Cells, NaturalMiceRNA, MessengerT-LymphocytesNatural killer (NK) cells recognize and kill target cells undergoing different types of stress. NK cells are also capable of modulating immune responses. In particular, they regulate T cell functions. Small RNA next-generation sequencing of resting and activated human NK cells and their secreted extracellular vesicles (EVs) led to the identification of a specific repertoire of NK-EV-associated microRNAs and their post-transcriptional modifications signature. Several microRNAs of NK-EVs, namely miR-10b-5p, miR-92a-3p, and miR-155-5p, specifically target molecules involved in Th1 responses. NK-EVs promote the downregulation of GATA3 mRNA in CD4+ T cells and subsequent TBX21 de-repression that leads to Th1 polarization and IFN-γ and IL-2 production. NK-EVs also have an effect on monocyte and moDCs (monocyte-derived dendritic cells) function, driving their activation and increased presentation and costimulatory functions. Nanoparticle-delivered NK-EV microRNAs partially recapitulate NK-EV effects in mice. Our results provide new insights on the immunomodulatory roles of NK-EVs that may help to improve their use as immunotherapeutic tools.eLife Sciences PublicationsFundación BBVAComunidad de Madrid (España)Ministerio de Economía y Competitividad (España)Instituto de Salud Carlos IIIFundación Ramón ArecesMinisterio de Ciencia, Innovación y Universidades (España)Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)20232023-03-1720222022-07-2920222022-07-29journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/15670reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/156702026-06-12T12:43:37Z
dc.title.none.fl_str_mv Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.
title Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.
spellingShingle Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.
Dosil, Sara G
Extracellular Vesicles
MicroRNAs
Animals
Humans
Killer Cells, Natural
Mice
RNA, Messenger
T-Lymphocytes
title_short Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.
title_full Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.
title_fullStr Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.
title_full_unstemmed Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.
title_sort Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.
dc.creator.none.fl_str_mv Dosil, Sara G
Lopez-Cobo, Sheila
Rodriguez-Galan, Ana
Fernandez-Delgado, Irene
Ramirez-Huesca, Marta
Milan-Rois, Paula
Castellanos, Milagros
Somoza, Alvaro
Gómez, Manuel José
Reyburn, Hugh T
Vales-Gomez, Mar
Sánchez Madrid, Francisco
Fernandez-Messina, Lola
author Dosil, Sara G
author_facet Dosil, Sara G
Lopez-Cobo, Sheila
Rodriguez-Galan, Ana
Fernandez-Delgado, Irene
Ramirez-Huesca, Marta
Milan-Rois, Paula
Castellanos, Milagros
Somoza, Alvaro
Gómez, Manuel José
Reyburn, Hugh T
Vales-Gomez, Mar
Sánchez Madrid, Francisco
Fernandez-Messina, Lola
author_role author
author2 Lopez-Cobo, Sheila
Rodriguez-Galan, Ana
Fernandez-Delgado, Irene
Ramirez-Huesca, Marta
Milan-Rois, Paula
Castellanos, Milagros
Somoza, Alvaro
Gómez, Manuel José
Reyburn, Hugh T
Vales-Gomez, Mar
Sánchez Madrid, Francisco
Fernandez-Messina, Lola
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundación BBVA
Comunidad de Madrid (España)
Ministerio de Economía y Competitividad (España)
Instituto de Salud Carlos III
Fundación Ramón Areces
Ministerio de Ciencia, Innovación y Universidades (España)
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
dc.subject.none.fl_str_mv Extracellular Vesicles
MicroRNAs
Animals
Humans
Killer Cells, Natural
Mice
RNA, Messenger
T-Lymphocytes
topic Extracellular Vesicles
MicroRNAs
Animals
Humans
Killer Cells, Natural
Mice
RNA, Messenger
T-Lymphocytes
description Natural killer (NK) cells recognize and kill target cells undergoing different types of stress. NK cells are also capable of modulating immune responses. In particular, they regulate T cell functions. Small RNA next-generation sequencing of resting and activated human NK cells and their secreted extracellular vesicles (EVs) led to the identification of a specific repertoire of NK-EV-associated microRNAs and their post-transcriptional modifications signature. Several microRNAs of NK-EVs, namely miR-10b-5p, miR-92a-3p, and miR-155-5p, specifically target molecules involved in Th1 responses. NK-EVs promote the downregulation of GATA3 mRNA in CD4+ T cells and subsequent TBX21 de-repression that leads to Th1 polarization and IFN-γ and IL-2 production. NK-EVs also have an effect on monocyte and moDCs (monocyte-derived dendritic cells) function, driving their activation and increased presentation and costimulatory functions. Nanoparticle-delivered NK-EV microRNAs partially recapitulate NK-EV effects in mice. Our results provide new insights on the immunomodulatory roles of NK-EVs that may help to improve their use as immunotherapeutic tools.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-07-29
2022
2022-07-29
2023
2023-03-17
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/15670
url http://hdl.handle.net/20.500.12105/15670
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419450661339136
score 15,811543

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