Metabolic adaptations in skeletal muscle after 84 days of bed rest with and without concurrent flywheel resistance exercise

As metabolic changes in human skeletal muscle after long-term (simulated) spaceflight are not well understood, this study examined the effects of long-term microgravity, with and without concurrent resistance exercise, on skeletal muscle oxidative and glycolytic capacity. Twenty-one men were subject...

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Autores: Irimia, José M., Guerrero, Mario, Rodriguez-Miguelez, Paula, Cadefau Surroca, Joan Aureli, Tesch, Per T., Cussó Fresquet, M. Roser, Fernandez-Gonzalo, Rodrigo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/125287
Acceso en línea:https://hdl.handle.net/2445/125287
Access Level:acceso abierto
Palabra clave:Metabolisme energètic
Músculs
Exercici terapèutic
Adaptació (Fisiologia)
Energy metabolism
Muscles
Exercise therapy
Adaptation (Physiology)
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spelling Metabolic adaptations in skeletal muscle after 84 days of bed rest with and without concurrent flywheel resistance exerciseIrimia, José M.Guerrero, MarioRodriguez-Miguelez, PaulaCadefau Surroca, Joan AureliTesch, Per T.Cussó Fresquet, M. RoserFernandez-Gonzalo, RodrigoMetabolisme energèticMúsculsExercici terapèuticAdaptació (Fisiologia)Energy metabolismMusclesExercise therapyAdaptation (Physiology)As metabolic changes in human skeletal muscle after long-term (simulated) spaceflight are not well understood, this study examined the effects of long-term microgravity, with and without concurrent resistance exercise, on skeletal muscle oxidative and glycolytic capacity. Twenty-one men were subjected to 84 days head-down tilt bed rest with (BRE; n 9) or without (BR; n 12) concurrent flywheel resistance exercise. Activity and gene expression of glycogen synthase, glycogen phosphorylase (GPh), hexokinase, phosphofructokinase-1 (PFK-1), and citrate synthase (CS), as well as gene expression of succinate dehydrogenase (SDH), vascular endothelial growth factor (VEFG), peroxisome proliferator-activated receptor gamma coactivator- 1 (PGC-1 ), and myostatin, were analyzed in samples from m.vastus lateralis collected before and after bed rest. Activity and gene expression of enzymes controlling oxidative metabolism (CS, SDH) decreased in BR but were partially maintained in BRE. Activity of enzymes regulating anaerobic glycolysis (GPh, PFK-1) was unchanged in BR. Resistance exercise increased the activity of GPh. PGC-1 and VEGF expression decreased in both BR and BRE. Myostatin increased in BR but decreased in BRE after bed rest. The analyses of these unique samples indicate that long-term microgravity induces marked alterations in the oxidative, but not the glycolytic, energy system. The proposed flywheel resistance exercise was effective in counteracting some of the metabolic alterations triggered by 84-day bed rest. Given the disparity between gene expression vs. enzyme activity in several key metabolic markers, posttranscriptional mechanisms should be explored to fully evaluate metabolic adaptations to long-term microgravity with/without exercise countermeasures in human skeletal muscle.American Physiological Society2018201820172018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion44 p.application/pdfhttps://hdl.handle.net/2445/125287Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1152/japplphysiol.00521.2016Journal of Applied Physiology, 2017, vol. 122, num. 1, p. 96-103https://doi.org/10.1152/japplphysiol.00521.2016(c) American Physiological Society, 2017info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1252872026-05-29T05:05:01Z
dc.title.none.fl_str_mv Metabolic adaptations in skeletal muscle after 84 days of bed rest with and without concurrent flywheel resistance exercise
title Metabolic adaptations in skeletal muscle after 84 days of bed rest with and without concurrent flywheel resistance exercise
spellingShingle Metabolic adaptations in skeletal muscle after 84 days of bed rest with and without concurrent flywheel resistance exercise
Irimia, José M.
Metabolisme energètic
Músculs
Exercici terapèutic
Adaptació (Fisiologia)
Energy metabolism
Muscles
Exercise therapy
Adaptation (Physiology)
title_short Metabolic adaptations in skeletal muscle after 84 days of bed rest with and without concurrent flywheel resistance exercise
title_full Metabolic adaptations in skeletal muscle after 84 days of bed rest with and without concurrent flywheel resistance exercise
title_fullStr Metabolic adaptations in skeletal muscle after 84 days of bed rest with and without concurrent flywheel resistance exercise
title_full_unstemmed Metabolic adaptations in skeletal muscle after 84 days of bed rest with and without concurrent flywheel resistance exercise
title_sort Metabolic adaptations in skeletal muscle after 84 days of bed rest with and without concurrent flywheel resistance exercise
dc.creator.none.fl_str_mv Irimia, José M.
Guerrero, Mario
Rodriguez-Miguelez, Paula
Cadefau Surroca, Joan Aureli
Tesch, Per T.
Cussó Fresquet, M. Roser
Fernandez-Gonzalo, Rodrigo
author Irimia, José M.
author_facet Irimia, José M.
Guerrero, Mario
Rodriguez-Miguelez, Paula
Cadefau Surroca, Joan Aureli
Tesch, Per T.
Cussó Fresquet, M. Roser
Fernandez-Gonzalo, Rodrigo
author_role author
author2 Guerrero, Mario
Rodriguez-Miguelez, Paula
Cadefau Surroca, Joan Aureli
Tesch, Per T.
Cussó Fresquet, M. Roser
Fernandez-Gonzalo, Rodrigo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Metabolisme energètic
Músculs
Exercici terapèutic
Adaptació (Fisiologia)
Energy metabolism
Muscles
Exercise therapy
Adaptation (Physiology)
topic Metabolisme energètic
Músculs
Exercici terapèutic
Adaptació (Fisiologia)
Energy metabolism
Muscles
Exercise therapy
Adaptation (Physiology)
description As metabolic changes in human skeletal muscle after long-term (simulated) spaceflight are not well understood, this study examined the effects of long-term microgravity, with and without concurrent resistance exercise, on skeletal muscle oxidative and glycolytic capacity. Twenty-one men were subjected to 84 days head-down tilt bed rest with (BRE; n 9) or without (BR; n 12) concurrent flywheel resistance exercise. Activity and gene expression of glycogen synthase, glycogen phosphorylase (GPh), hexokinase, phosphofructokinase-1 (PFK-1), and citrate synthase (CS), as well as gene expression of succinate dehydrogenase (SDH), vascular endothelial growth factor (VEFG), peroxisome proliferator-activated receptor gamma coactivator- 1 (PGC-1 ), and myostatin, were analyzed in samples from m.vastus lateralis collected before and after bed rest. Activity and gene expression of enzymes controlling oxidative metabolism (CS, SDH) decreased in BR but were partially maintained in BRE. Activity of enzymes regulating anaerobic glycolysis (GPh, PFK-1) was unchanged in BR. Resistance exercise increased the activity of GPh. PGC-1 and VEGF expression decreased in both BR and BRE. Myostatin increased in BR but decreased in BRE after bed rest. The analyses of these unique samples indicate that long-term microgravity induces marked alterations in the oxidative, but not the glycolytic, energy system. The proposed flywheel resistance exercise was effective in counteracting some of the metabolic alterations triggered by 84-day bed rest. Given the disparity between gene expression vs. enzyme activity in several key metabolic markers, posttranscriptional mechanisms should be explored to fully evaluate metabolic adaptations to long-term microgravity with/without exercise countermeasures in human skeletal muscle.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/125287
url https://hdl.handle.net/2445/125287
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1152/japplphysiol.00521.2016
Journal of Applied Physiology, 2017, vol. 122, num. 1, p. 96-103
https://doi.org/10.1152/japplphysiol.00521.2016
dc.rights.none.fl_str_mv (c) American Physiological Society, 2017
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Physiological Society, 2017
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 44 p.
application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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