Fecundity, in vitro early larval development and karyotype of the zoonotic nematode Anisakis pegreffii

The in vitro life cycle of zoonotic helminths is an essential tool for -omic translational studies focused on disease control and treatment. Anisakiosis is an emerging zoonosis contracted by the ingestion of raw or undercooked fish infected with the third stage larvae (L3) of two sibling species Ani...

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Autores: Moratal Martínez, Samantha, Zrzavá, Magda, Hrabar, Jerko, Dea-Ayuela, María Auxiliadora, López-Ramon, Jordi, Mladineo, Ivona
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Católica de Valencia San Vicente Mártir
Repositorio:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
Idioma:inglés
OAI Identifier:oai:riucv.ucv.es:20.500.12466/7254
Acceso en línea:https://hdl.handle.net/20.500.12466/7254
Access Level:acceso abierto
Palabra clave:Anisakis pegreffii
Anisakis simplex
Fecundity in vitro
Karyotype
Ultrastructure
3109 Ciencias Veterinarias
2401.12 Parasitología Animal
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spelling Fecundity, in vitro early larval development and karyotype of the zoonotic nematode Anisakis pegreffiiMoratal Martínez, SamanthaZrzavá, MagdaHrabar, JerkoDea-Ayuela, María AuxiliadoraLópez-Ramon, JordiMladineo, IvonaAnisakis pegreffiiAnisakis simplexFecundity in vitroKaryotypeUltrastructure3109 Ciencias Veterinarias2401.12 Parasitología AnimalThe in vitro life cycle of zoonotic helminths is an essential tool for -omic translational studies focused on disease control and treatment. Anisakiosis is an emerging zoonosis contracted by the ingestion of raw or undercooked fish infected with the third stage larvae (L3) of two sibling species Anisakis simplex sensu stricto (s.s.) and Anisakis pegreffii, the latter being the predominant species in the Mediterranean basin. Recently, in vitro culture of A. pegreffii has been developed to enable fast and large-scale production of fertile adults. However, the conditions for larval development from hatching to infective L3 were not fulfilled to complete the cycle. Herein, we used a Drosophila medium supplemented with chicken serum and adjusted different osmolarities to maintain the culture of L3 hatched from eggs for up to 17 weeks. The highest survival rate was observed in the medium with the highest osmolarities, which also allowed the highest larval exsheathment rate. Key morphological features of embryogenesis and postembryogenesis studied by transmission electron microscopy revealed that the excretory gland cell is differentiated already up to 48 h post-hatching. Extracellular vesicles and cell-free mitochondria are discharged between the two cuticle sheets of the second stage larvae (L2). Contemporarly cultivated, two populations of adult A. simplex s.s. and A. pegreffii reached an average production of 29,914.05 (± 27,629.36) and 24,370.96 (± 12,564.86) eggs/day/female, respectively. The chromosome spreads of A. pegreffii obtained from mature gonads suggests a diploid karyotype formula of 2n = 18. The development of a reliable protocol for the in vitro culture of a polyxenous nematode such as Anisakis spp. will serve to screen for much needed novel drug targets, but also to study the intricated and unknown ecological and physiological traits of these trophically transmitted marine nematodes.20252025-12-2920232023-11-0120232023-11-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12466/7254reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártirinstname:Universidad Católica de Valencia San Vicente MártirInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:riucv.ucv.es:20.500.12466/72542026-06-19T08:32:07Z
dc.title.none.fl_str_mv Fecundity, in vitro early larval development and karyotype of the zoonotic nematode Anisakis pegreffii
title Fecundity, in vitro early larval development and karyotype of the zoonotic nematode Anisakis pegreffii
spellingShingle Fecundity, in vitro early larval development and karyotype of the zoonotic nematode Anisakis pegreffii
Moratal Martínez, Samantha
Anisakis pegreffii
Anisakis simplex
Fecundity in vitro
Karyotype
Ultrastructure
3109 Ciencias Veterinarias
2401.12 Parasitología Animal
title_short Fecundity, in vitro early larval development and karyotype of the zoonotic nematode Anisakis pegreffii
title_full Fecundity, in vitro early larval development and karyotype of the zoonotic nematode Anisakis pegreffii
title_fullStr Fecundity, in vitro early larval development and karyotype of the zoonotic nematode Anisakis pegreffii
title_full_unstemmed Fecundity, in vitro early larval development and karyotype of the zoonotic nematode Anisakis pegreffii
title_sort Fecundity, in vitro early larval development and karyotype of the zoonotic nematode Anisakis pegreffii
dc.creator.none.fl_str_mv Moratal Martínez, Samantha
Zrzavá, Magda
Hrabar, Jerko
Dea-Ayuela, María Auxiliadora
López-Ramon, Jordi
Mladineo, Ivona
author Moratal Martínez, Samantha
author_facet Moratal Martínez, Samantha
Zrzavá, Magda
Hrabar, Jerko
Dea-Ayuela, María Auxiliadora
López-Ramon, Jordi
Mladineo, Ivona
author_role author
author2 Zrzavá, Magda
Hrabar, Jerko
Dea-Ayuela, María Auxiliadora
López-Ramon, Jordi
Mladineo, Ivona
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Anisakis pegreffii
Anisakis simplex
Fecundity in vitro
Karyotype
Ultrastructure
3109 Ciencias Veterinarias
2401.12 Parasitología Animal
topic Anisakis pegreffii
Anisakis simplex
Fecundity in vitro
Karyotype
Ultrastructure
3109 Ciencias Veterinarias
2401.12 Parasitología Animal
description The in vitro life cycle of zoonotic helminths is an essential tool for -omic translational studies focused on disease control and treatment. Anisakiosis is an emerging zoonosis contracted by the ingestion of raw or undercooked fish infected with the third stage larvae (L3) of two sibling species Anisakis simplex sensu stricto (s.s.) and Anisakis pegreffii, the latter being the predominant species in the Mediterranean basin. Recently, in vitro culture of A. pegreffii has been developed to enable fast and large-scale production of fertile adults. However, the conditions for larval development from hatching to infective L3 were not fulfilled to complete the cycle. Herein, we used a Drosophila medium supplemented with chicken serum and adjusted different osmolarities to maintain the culture of L3 hatched from eggs for up to 17 weeks. The highest survival rate was observed in the medium with the highest osmolarities, which also allowed the highest larval exsheathment rate. Key morphological features of embryogenesis and postembryogenesis studied by transmission electron microscopy revealed that the excretory gland cell is differentiated already up to 48 h post-hatching. Extracellular vesicles and cell-free mitochondria are discharged between the two cuticle sheets of the second stage larvae (L2). Contemporarly cultivated, two populations of adult A. simplex s.s. and A. pegreffii reached an average production of 29,914.05 (± 27,629.36) and 24,370.96 (± 12,564.86) eggs/day/female, respectively. The chromosome spreads of A. pegreffii obtained from mature gonads suggests a diploid karyotype formula of 2n = 18. The development of a reliable protocol for the in vitro culture of a polyxenous nematode such as Anisakis spp. will serve to screen for much needed novel drug targets, but also to study the intricated and unknown ecological and physiological traits of these trophically transmitted marine nematodes.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-11-01
2023
2023-11-01
2025
2025-12-29
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12466/7254
url https://hdl.handle.net/20.500.12466/7254
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
instname:Universidad Católica de Valencia San Vicente Mártir
instname_str Universidad Católica de Valencia San Vicente Mártir
reponame_str RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
collection RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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