Consequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattle

The aim of this study was to evaluate the potential effects of methyl donor supplementation of pregnant animals in the presence or absence of a concomitant lactation on the methylome of the offspring. Twenty Holstein cows, 10 nulliparous (non-lactating while pregnant) and 10 multiparous (lactating w...

Descripción completa

Detalles Bibliográficos
Autores: Bach, Alex, Aris, Anna, Guasch, Isabel
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/1839
Acceso en línea:http://hdl.handle.net/20.500.12327/1839
https://doi.org/10.1371/journal.pone.0189581
Access Level:acceso abierto
Palabra clave:636
id ES_c9b4dc798f24ba2912cd8dc38fcfecc4
oai_identifier_str oai:recercat.cat:20.500.12327/1839
network_acronym_str ES
network_name_str España
repository_id_str
spelling Consequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattleBach, AlexAris, AnnaGuasch, Isabel636The aim of this study was to evaluate the potential effects of methyl donor supplementation of pregnant animals in the presence or absence of a concomitant lactation on the methylome of the offspring. Twenty Holstein cows, 10 nulliparous (non-lactating while pregnant) and 10 multiparous (lactating while pregnant) were blocked by parity and randomly assigned to an i.m. weekly injections of a placebo (CTRL) or a solution containing methyl donors (MET). After calving, 5 calves randomly selected from each treatment (two born to non-lactating and three to lactating dams) were blood-sampled to determine their full methylome. There were more than 2,000 CpG differentially methylated between calves born to CTRL and those born to MET, and also between calves born to lactating and non-lactating dams. Most of the differences affected genes involved in immune function, cell growth regulation and differentiation, kinase activity, and ion channeling. We conclude that the coexistence of pregnancy and lactation affects the methylome of the offspring, and that supplementation of methyl donors early in gestation has also consequences on the methylome.info:eu-repo/semantics/publishedVersionPublic Library of ScienceProducció AnimalProducció de Remugants2017info:eu-repo/semantics/article12http://hdl.handle.net/20.500.12327/1839https://doi.org/10.1371/journal.pone.0189581reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPLoS ONEMINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-38292/ES/CAMBIO DE PARADIGMA: PROGRAMACION FETAL Y POST-NATAL PARA MEJORAR EL METABOLISMO INTERMEDIO DEL VACUNO LECHERO/Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/18392026-05-29T05:05:01Z
dc.title.none.fl_str_mv Consequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattle
title Consequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattle
spellingShingle Consequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattle
Bach, Alex
636
title_short Consequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattle
title_full Consequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattle
title_fullStr Consequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattle
title_full_unstemmed Consequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattle
title_sort Consequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattle
dc.creator.none.fl_str_mv Bach, Alex
Aris, Anna
Guasch, Isabel
author Bach, Alex
author_facet Bach, Alex
Aris, Anna
Guasch, Isabel
author_role author
author2 Aris, Anna
Guasch, Isabel
author2_role author
author
dc.contributor.none.fl_str_mv Producció Animal
Producció de Remugants
dc.subject.none.fl_str_mv 636
topic 636
description The aim of this study was to evaluate the potential effects of methyl donor supplementation of pregnant animals in the presence or absence of a concomitant lactation on the methylome of the offspring. Twenty Holstein cows, 10 nulliparous (non-lactating while pregnant) and 10 multiparous (lactating while pregnant) were blocked by parity and randomly assigned to an i.m. weekly injections of a placebo (CTRL) or a solution containing methyl donors (MET). After calving, 5 calves randomly selected from each treatment (two born to non-lactating and three to lactating dams) were blood-sampled to determine their full methylome. There were more than 2,000 CpG differentially methylated between calves born to CTRL and those born to MET, and also between calves born to lactating and non-lactating dams. Most of the differences affected genes involved in immune function, cell growth regulation and differentiation, kinase activity, and ion channeling. We conclude that the coexistence of pregnancy and lactation affects the methylome of the offspring, and that supplementation of methyl donors early in gestation has also consequences on the methylome.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/1839
https://doi.org/10.1371/journal.pone.0189581
url http://hdl.handle.net/20.500.12327/1839
https://doi.org/10.1371/journal.pone.0189581
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS ONE
MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-38292/ES/CAMBIO DE PARADIGMA: PROGRAMACION FETAL Y POST-NATAL PARA MEJORAR EL METABOLISMO INTERMEDIO DEL VACUNO LECHERO/
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419394101149696
score 15.812429

Similares en LA Referencia