NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source

17 Pág.

Detalhes bibliográficos
Autores: Ibarra, David, García-Fuentevilla, Luisa, Domínguez, Gabriela, Martín-Sampedro, Raquel, Hernández, Manuel, Arias, María Enriqueta, Santos, José I., Eugenio, María E.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/330212
Acesso em linha:http://hdl.handle.net/10261/330212
https://api.elsevier.com/content/abstract/scopus_id/85147894135
Access Level:acceso abierto
Palavra-chave:Kraft lignin
NMR characterization
Eucalypt
Laccase
Polymerization
id ES_c99cd616d869a7764cd9cf3923fddb42
oai_identifier_str oai:digital.csic.es:10261/330212
network_acronym_str ES
network_name_str España
repository_id_str
spelling NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes SourceIbarra, DavidGarcía-Fuentevilla, LuisaDomínguez, GabrielaMartín-Sampedro, RaquelHernández, ManuelArias, María EnriquetaSantos, José I.Eugenio, María E.Kraft ligninNMR characterizationEucalyptLaccasePolymerization17 Pág.The usage of laccases is a sustainable and environmentally friendly approach to modifying the Kraft lignin structure for use in certain applications. However, the inherent structure of Kraft lignin, as well as that resulting from laccase modification, still presents challenges for fundamental comprehension and successful lignin valorization. In this study, bacterial and fungal laccases were employed to modify eucalypt Kraft lignin. To evaluate the type and range of the chemical and structural changes of laccase-treated lignins, different NMR techniques, including solution 1H and 2D NMR (heteronuclear single quantum correlation (HSQC)), and solid-state 13C NMR, were applied. Size exclusion chromatography and infrared spectroscopy were also used. Interestingly, HSQC analysis showed substantial changes in the oxygenated aliphatic region of lignins, showing an almost complete absence of signals corresponding to side-chains due to laccase depolymerization. Simultaneously, a significant loss of aromatic signals was observed by HSQC and 1H NMR, which was attributed to a deprotonation of the lignin benzenic rings due to polymerization/condensation by laccase reactions. Then, condensed structures, such as α-5', 5-5', and 4-O-5', were detected by HSQC and 13C NMR, supporting the increment in molecular weight, as well as the phenolic content reduction determined in lignins.This research was funded by Comunidad de Madrid via Project SUSTEC-CM S2018/EMT-4348; MCINN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” via Project RTI2018-096080-B-C22; and MCINN via Project TED2021-132122B-C21.Peer reviewedMultidisciplinary Digital Publishing InstituteComunidad de MadridAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Ibarra Trejo, David [0000-0003-3001-7308]García-Fuentevilla, Luisa [0000-0001-8255-0097]Domínguez, Gabriela [0000-0001-7358-0913]Martín-Sampedro, Raquel [0000-0003-2083-3723]Arias, María Enriqueta [0000-0002-6804-1779]Santos, José I. [0000-0002-5199-2332]Eugenio, María E. [0000-0003-4550-5400]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/330212https://api.elsevier.com/content/abstract/scopus_id/85147894135reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#S2018/EMT-4348/SUSTEC-CMinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096080-B-C22info:eu-repo/grantAgreement/AEI//TED2021-132122B-C21Instituto de Ciencias Forestales (ICIFOR)https://doi.org/10.3390/ijms24032359Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3302122026-05-22T06:33:51Z
dc.title.none.fl_str_mv NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source
title NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source
spellingShingle NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source
Ibarra, David
Kraft lignin
NMR characterization
Eucalypt
Laccase
Polymerization
title_short NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source
title_full NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source
title_fullStr NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source
title_full_unstemmed NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source
title_sort NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source
dc.creator.none.fl_str_mv Ibarra, David
García-Fuentevilla, Luisa
Domínguez, Gabriela
Martín-Sampedro, Raquel
Hernández, Manuel
Arias, María Enriqueta
Santos, José I.
Eugenio, María E.
author Ibarra, David
author_facet Ibarra, David
García-Fuentevilla, Luisa
Domínguez, Gabriela
Martín-Sampedro, Raquel
Hernández, Manuel
Arias, María Enriqueta
Santos, José I.
Eugenio, María E.
author_role author
author2 García-Fuentevilla, Luisa
Domínguez, Gabriela
Martín-Sampedro, Raquel
Hernández, Manuel
Arias, María Enriqueta
Santos, José I.
Eugenio, María E.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Comunidad de Madrid
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Ibarra Trejo, David [0000-0003-3001-7308]
García-Fuentevilla, Luisa [0000-0001-8255-0097]
Domínguez, Gabriela [0000-0001-7358-0913]
Martín-Sampedro, Raquel [0000-0003-2083-3723]
Arias, María Enriqueta [0000-0002-6804-1779]
Santos, José I. [0000-0002-5199-2332]
Eugenio, María E. [0000-0003-4550-5400]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Kraft lignin
NMR characterization
Eucalypt
Laccase
Polymerization
topic Kraft lignin
NMR characterization
Eucalypt
Laccase
Polymerization
description 17 Pág.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/330212
https://api.elsevier.com/content/abstract/scopus_id/85147894135
url http://hdl.handle.net/10261/330212
https://api.elsevier.com/content/abstract/scopus_id/85147894135
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
S2018/EMT-4348/SUSTEC-CM
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096080-B-C22
info:eu-repo/grantAgreement/AEI//TED2021-132122B-C21
Instituto de Ciencias Forestales (ICIFOR)
https://doi.org/10.3390/ijms24032359
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419385713590272
score 15,812429

Registos relacionados